Literature DB >> 2186363

The initiation of translation in E. coli: apparent base pairing between the 16srRNA and downstream sequences of the mRNA.

M L Sprengart1, H P Fatscher, E Fuchs.   

Abstract

Bacteriophage T7's gene 0.3, coding for an antirestriction protein, possesses one of the strongest translation initiation regions (TIR) in E. coli. It was isolated on DNA fragments of differing length and cloned upstream of the mouse dihydrofolate reductase gene in an expression vector to control the translation of this gene's sequence. The TIR's efficiency was highly dependent on nucleotides +15 to +26 downstream of the gene's AUG. This sequence is complementary to nucleotides 1471-1482 of the 16srRNA. Similar sequences complementary to this rRNA region are present in other efficient TIRs of the E. coli genome and those of its bacteriophages. There seems to be a correlation between this sequence homology and the efficiency of the initiation signals. We propose that this region specifies a stimulatory interaction between the mRNA and 16srRNA besides the Shine-Dalgarno interaction during the translation initiation step.

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Year:  1990        PMID： 2186363      PMCID： PMC330588          DOI： 10.1093/nar/18.7.1719

Source DB:  PubMed          Journal:  Nucleic Acids Res        ISSN： 0305-1048            Impact factor:   16.971


  38 in total

1.  Lambda repressor regulates the switch between PR and Prm promoters.

Authors:  A Walz; V Pirrotta; K Ineichen
Journal:  Nature       Date:  1976-08-19       Impact factor: 49.962

2.  Optimal computer folding of large RNA sequences using thermodynamics and auxiliary information.

Authors:  M Zuker; P Stiegler
Journal:  Nucleic Acids Res       Date:  1981-01-10       Impact factor: 16.971

3.  Secondary structure of 16S ribosomal RNA.

Authors:  H F Noller; C R Woese
Journal:  Science       Date:  1981-04-24       Impact factor: 47.728

4.  Codon usage in bacteria: correlation with gene expressivity.

Authors:  M Gouy; C Gautier
Journal:  Nucleic Acids Res       Date:  1982-11-25       Impact factor: 16.971

5.  Characterization of translational initiation sites in E. coli.

Authors:  G D Stormo; T D Schneider; L M Gold
Journal:  Nucleic Acids Res       Date:  1982-05-11       Impact factor: 16.971

6.  Nucleic acid helix-unwinding properties of ribosomal protein S1 and the role of S1 in mRNA binding to ribosomes.

Authors:  A Kolb; J M Hermoso; J O Thomas; W Szer
Journal:  Proc Natl Acad Sci U S A       Date:  1977-06       Impact factor: 11.205

7.  Segmental flexibility in Escherichia coli ribosomal protein S1 as studied by fluorescence polarization.

Authors:  Y G Chu; C R Cantor
Journal:  Nucleic Acids Res       Date:  1979       Impact factor: 16.971

8.  Purification of the gene 0.3 protein of bacteriophage T7, an inhibitor of the DNA restriction system of Escherichia coli.

Authors:  K K Mark; F W Studier
Journal:  J Biol Chem       Date:  1981-03-10       Impact factor: 5.157

9.  A system for shotgun DNA sequencing.

Authors:  J Messing; R Crea; P H Seeburg
Journal:  Nucleic Acids Res       Date:  1981-01-24       Impact factor: 16.971

10.  DNA sequencing with chain-terminating inhibitors.

Authors:  F Sanger; S Nicklen; A R Coulson
Journal:  Proc Natl Acad Sci U S A       Date:  1977-12       Impact factor: 11.205
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  68 in total

1.  The initiation codon affects ribosome binding and translational efficiency in Escherichia coli of cI mRNA with or without the 5' untranslated leader.

Authors:  S M O'Donnell; G R Janssen
Journal:  J Bacteriol       Date:  2001-02       Impact factor: 3.490

2.  Enhancement of translation by the downstream box does not involve base pairing of mRNA with the penultimate stem sequence of 16S rRNA.

Authors:  M O'Connor; T Asai; C L Squires; A E Dahlberg
Journal:  Proc Natl Acad Sci U S A       Date:  1999-08-03       Impact factor: 11.205

3.  Translational induction of heat shock transcription factor sigma32: evidence for a built-in RNA thermosensor.

Authors:  M T Morita; Y Tanaka; T S Kodama; Y Kyogoku; H Yanagi; T Yura
Journal:  Genes Dev       Date:  1999-03-15       Impact factor: 11.361

4.  Determinants on tmRNA for initiating efficient and precise trans-translation: some mutations upstream of the tag-encoding sequence of Escherichia coli tmRNA shift the initiation point of trans-translation in vitro.

Authors:  S Lee; M Ishii; T Tadaki; A Muto; H Himeno
Journal:  RNA       Date:  2001-07       Impact factor: 4.942

5.  Characterization of the sat operon in Streptococcus mutans: evidence for a role of Ffh in acid tolerance.

Authors:  B H Kremer; M van der Kraan; P J Crowley; I R Hamilton; L J Brady; A S Bleiweis
Journal:  J Bacteriol       Date:  2001-04       Impact factor: 3.490

6.  Evidence for the frequent use of TTG as the translation initiation codon of mitochondrial protein genes in the nematodes, Ascaris suum and Caenorhabditis elegans.

Authors:  R Okimoto; J L Macfarlane; D R Wolstenholme
Journal:  Nucleic Acids Res       Date:  1990-10-25       Impact factor: 16.971

7.  Construction and use of lambda PL promoter vectors for direct cloning and high level expression of PCR amplified DNA coding sequences.

Authors:  X Cheng; T A Patterson
Journal:  Nucleic Acids Res       Date:  1992-09-11       Impact factor: 16.971

8.  A codon window in mRNA downstream of the initiation codon where NGG codons give strongly reduced gene expression in Escherichia coli.

Authors:  Ernesto I Gonzalez de Valdivia; Leif A Isaksson
Journal:  Nucleic Acids Res       Date:  2004-09-30       Impact factor: 16.971

9.  Mutational analysis of cat-86 gene expression controlled by lactococcal promoters in Lactococcus lactis subsp. lactis and Escherichia coli.

Authors:  B Bojovic; G Djordjevic; A Banina; L Topisirovic
Journal:  J Bacteriol       Date:  1994-11       Impact factor: 3.490

10.  A novel RNA product of the tyrT operon of Escherichia coli.

Authors:  M Bösl; H Kersten
Journal:  Nucleic Acids Res       Date:  1991-11-11       Impact factor: 16.971
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