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Literature DB >> 2183182

Cloning and nucleotide sequence of the gene encoding the Ecal DNA methyltransferase.

Abstract

The gene coding for the GGTNACC specific Ecal DNA methyltransferase (M.Ecal) has been cloned in E. coli from Enterobacter cloacae and its nucleotide sequence has been determined. The ecalM gene codes for a protein of 452 amino acids (Mr: 51,111). It was determined that M.Ecal is an adenine methyltransferase. M.Ecal shows limited amino acid sequence similarity to other adenine methyltransferases. A clone that expresses Ecal methyltransferase at high level was constructed.

Entities: Gene Species

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Year: 1990 PMID： 2183182 PMCID： PMC330275 DOI： 10.1093/nar/18.2.355

Source DB: PubMed Journal: Nucleic Acids Res ISSN： 0305-1048 Impact factor: 16.971

40 in total

1. Nucleotide sequence of the FokI restriction-modification system: separate strand-specificity domains in the methyltransferase.

Authors: M C Looney; L S Moran; W E Jack; G R Feehery; J S Benner; B E Slatko; G G Wilson
Journal: Gene Date: 1989-08-15 Impact factor: 3.688

2. Nucleotide sequence of bacteriophage lambda DNA.

Authors: F Sanger; A R Coulson; G F Hong; D F Hill; G B Petersen
Journal: J Mol Biol Date: 1982-12-25 Impact factor: 5.469

3. Sequence analysis of the DNA encoding the Eco RI endonuclease and methylase.

Authors: P J Greene; M Gupta; H W Boyer; W E Brown; J M Rosenberg
Journal: J Biol Chem Date: 1981-03-10 Impact factor: 5.157

4. DNA sequences of structural genes for Eco RI DNA restriction and modification enzymes.

Authors: A K Newman; R A Rubin; S H Kim; P Modrich
Journal: J Biol Chem Date: 1981-03-10 Impact factor: 5.157

5. New M13 vectors for cloning.

Authors: J Messing
Journal: Methods Enzymol Date: 1983 Impact factor: 1.600

6. Buffer gradient gels and 35S label as an aid to rapid DNA sequence determination.

Authors: M D Biggin; T J Gibson; G F Hong
Journal: Proc Natl Acad Sci U S A Date: 1983-07 Impact factor: 11.205

7. DNA sequencing with chain-terminating inhibitors.

Authors: F Sanger; S Nicklen; A R Coulson
Journal: Proc Natl Acad Sci U S A Date: 1977-12 Impact factor: 11.205

8. Restriction endonuclease EcaI from Enterobacter cloacae.

Authors: G Hobom; E Schwarz; M Melzer; H Mayer
Journal: Nucleic Acids Res Date: 1981-10-10 Impact factor: 16.971

9. The nucleotide sequence of the HhaII restriction and modification genes from Haemophilus haemolyticus.

Authors: B Schoner; S Kelly; H O Smith
Journal: Gene Date: 1983-10 Impact factor: 3.688

10. The isolation and characterization of the Escherichia coli DNA adenine methylase (dam) gene.

Authors: J E Brooks; R M Blumenthal; T R Gingeras
Journal: Nucleic Acids Res Date: 1983-02-11 Impact factor: 16.971

12 in total

1. Cloning and sequence analysis of the genes coding for Eco57I type IV restriction-modification enzymes.

Authors: A Janulaitis; R Vaisvila; A Timinskas; S Klimasauskas; V Butkus
Journal: Nucleic Acids Res Date: 1992-11-25 Impact factor: 16.971

2. Effect of site-specific methylation on DNA modification methyltransferases and restriction endonucleases.

Authors: M McClelland; M Nelson
Journal: Nucleic Acids Res Date: 1992-05-11 Impact factor: 16.971

3. Cloning and expression of the HpaI restriction-modification genes.

Authors: H Ito; H Shimato; A Sadaoka; H Kotani; F Kimizuka; I Kato
Journal: Nucleic Acids Res Date: 1992-02-25 Impact factor: 16.971

Review 4. Organization of restriction-modification systems.

Authors: G G Wilson
Journal: Nucleic Acids Res Date: 1991-05-25 Impact factor: 16.971

5. Site-specific methylation: effect on DNA modification methyltransferases and restriction endonucleases.

Authors: M Nelson; M McClelland
Journal: Nucleic Acids Res Date: 1991-04-25 Impact factor: 16.971

6. Genetic organization of the KpnI restriction--modification system.

Authors: D K Chatterjee; A W Hammond; R W Blakesley; S M Adams; G F Gerard
Journal: Nucleic Acids Res Date: 1991-12-11 Impact factor: 16.971

7. Characterization of the cloned BamHI restriction modification system: its nucleotide sequence, properties of the methylase, and expression in heterologous hosts.

Authors: J E Brooks; P D Nathan; D Landry; L A Sznyter; P Waite-Rees; C L Ives; L S Moran; B E Slatko; J S Benner
Journal: Nucleic Acids Res Date: 1991-02-25 Impact factor: 16.971