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Literature DB >> 1542567

Cloning and expression of the HpaI restriction-modification genes.

H Ito¹, H Shimato, A Sadaoka, H Kotani, F Kimizuka, I Kato.

Abstract

The genes from Haemophilus parainfluenzae encoding the HpaI restriction-modification system were cloned and expressed in Escherichia coli. From the DNA sequence, we predicted the HpaI endonuclease (R.HpaI) to have 254 amino acid residues (Mr 29,630) and the HpaI methyltransferase (M.HpaI) to have 314 amino acid residues (37,390). The R.HpaI and M.HpaI genes overlapped by 16 base pairs on the chromosomal DNA. The genes had the same orientation. The clone, named E. coli HB101-HPA2, overproduced R.HpaI. R.HpaI activity from the clone was 100-fold that from H. parainfluenzae. The amino acid sequence of M.HpaI was compared with those of other type II methyltransferases.

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Year: 1992 PMID： 1542567 PMCID： PMC312008 DOI： 10.1093/nar/20.4.705

Source DB: PubMed Journal: Nucleic Acids Res ISSN： 0305-1048 Impact factor: 16.971

40 in total

1. Detection of specific sequences among DNA fragments separated by gel electrophoresis.

Authors: E M Southern
Journal: J Mol Biol Date: 1975-11-05 Impact factor: 5.469

2. Characterization of the cloned BamHI restriction modification system: its nucleotide sequence, properties of the methylase, and expression in heterologous hosts.

Authors: J E Brooks; P D Nathan; D Landry; L A Sznyter; P Waite-Rees; C L Ives; L S Moran; B E Slatko; J S Benner
Journal: Nucleic Acids Res Date: 1991-02-25 Impact factor: 16.971

Cloning and expression of the HpaI restriction-modification genes.

1. Detection of specific sequences among DNA fragments separated by gel electrophoresis.

2. Characterization of the cloned BamHI restriction modification system: its nucleotide sequence, properties of the methylase, and expression in heterologous hosts.

3. Cloning and nucleotide sequence of the gene encoding the Ecal DNA methyltransferase.

4. A new method for isolation of a restriction enzyme from Hemophilus parainfluenzae.

5. Cleavage of structural proteins during the assembly of the head of bacteriophage T4.

6. Revised sequence of the tetracycline-resistance gene of pBR322.

7. Purification and properties of the Hpa I methylase.

8. Nucleotide sequence of the metH gene of Escherichia coli K-12 and comparison with that of Salmonella typhimurium LT2.

9. Studies on transformation of Escherichia coli with plasmids.

10. Cloning and expression of the Pst I restriction-modification system in Escherichia coli.

1. New nucleotide sequence data on the EMBL File Server.

2. Semisynthesis of cytotoxic proteins using a modified protein splicing element.

3. Effect of site-specific methylation on restriction endonucleases and DNA modification methyltransferases.

4. Effect of site-specific modification on restriction endonucleases and DNA modification methyltransferases.

5. BsuBI--an isospecific restriction and modification system of PstI: characterization of the BsuBI genes and enzymes.

6. Gene structure and expression of the MboI restriction--modification system.