Literature DB >> 6300769

The isolation and characterization of the Escherichia coli DNA adenine methylase (dam) gene.

J E Brooks, R M Blumenthal, T R Gingeras.   

Abstract

The E. coli dam (DNA adenine methylase) enzyme is known to methylate the sequence GATC. A general method for cloning sequence-specific DNA methylase genes was used to isolate the dam gene on a 1.14 kb fragment, inserted in the plasmid vector pBR322. Subsequent restriction mapping and subcloning experiments established a set of approximate boundaries of the gene. The nucleotide sequence of the dam gene was determined, and analysis of that sequence revealed a unique open reading frame which corresponded in length to that necessary to code for a protein the size of dam. Amino acid composition derived from this sequence corresponds closely to the amino acid composition of the purified dam protein. Enzymatic and DNA:DNA hybridization methods were used to investigate the possible presence of dam genes in a variety of prokaryotic organisms.

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Year:  1983        PMID： 6300769      PMCID： PMC325756          DOI： 10.1093/nar/11.3.837

Source DB:  PubMed          Journal:  Nucleic Acids Res        ISSN： 0305-1048            Impact factor:   16.971


  49 in total

1.  Location of DNA methylation genes on the Escherichia coli K-12 genetic map.

Authors:  M G Marinus
Journal:  Mol Gen Genet       Date:  1973-12-14

2.  Effect of growth conditions on the formation of the relaxation complex of supercoiled ColE1 deoxyribonucleic acid and protein in Escherichia coli.

Authors:  D B Clewell; D R Helinski
Journal:  J Bacteriol       Date:  1972-06       Impact factor: 3.490

3.  Detection of two restriction endonuclease activities in Haemophilus parainfluenzae using analytical agarose--ethidium bromide electrophoresis.

Authors:  P A Sharp; B Sugden; J Sambrook
Journal:  Biochemistry       Date:  1973-07-31       Impact factor: 3.162

4.  Enzymatic synthesis of deoxyribonucleic acid. XXXII. Replication of duplex deoxyribonucleic acid by polymerase at a single strand break.

Authors:  R B Kelly; N R Cozzarelli; M P Deutscher; I R Lehman; A Kornberg
Journal:  J Biol Chem       Date:  1970-01-10       Impact factor: 5.157

5.  A complementation analysis of the restriction and modification of DNA in Escherichia coli.

Authors:  H W Boyer; D Roulland-Dussoix
Journal:  J Mol Biol       Date:  1969-05-14       Impact factor: 5.469

6.  DNA methyltransferase-dependent transcription of the phage Mu mom gene.

Authors:  S Hattman
Journal:  Proc Natl Acad Sci U S A       Date:  1982-09       Impact factor: 11.205

7.  Primary structure of the chromosomal origins (oriC) of Enterobacter aerogenes and Klebsiella pneumoniae: comparisons and evolutionary relationships.

Authors:  J M Cleary; D W Smith; N E Harding; J W Zyskind
Journal:  J Bacteriol       Date:  1982-06       Impact factor: 3.490

8.  The nucleotide sequence of the structural gene for Escherichia coli tryptophanyl-tRNA synthetase.

Authors:  C V Hall; M vanCleemput; K H Muench; C Yanofsky
Journal:  J Biol Chem       Date:  1982-06-10       Impact factor: 5.157

9.  Nonchromosomal antibiotic resistance in bacteria: genetic transformation of Escherichia coli by R-factor DNA.

Authors:  S N Cohen; A C Chang; L Hsu
Journal:  Proc Natl Acad Sci U S A       Date:  1972-08       Impact factor: 11.205

10.  Isolation of deoxyribonucleic acid methylase mutants of Escherichia coli K-12.

Authors:  M G Marinus; N R Morris
Journal:  J Bacteriol       Date:  1973-06       Impact factor: 3.490
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  78 in total

Review 1.  Bacterial DNA methylation: a cell cycle regulator?

Authors:  A Reisenauer; L S Kahng; S McCollum; L Shapiro
Journal:  J Bacteriol       Date:  1999-09       Impact factor: 3.490

2.  The eclipse period of Escherichia coli.

Authors:  U von Freiesleben; M A Krekling; F G Hansen; A Løbner-Olesen
Journal:  EMBO J       Date:  2000-11-15       Impact factor: 11.598

3.  Stable co-existence of separate replicons in Escherichia coli is dependent on once-per-cell-cycle initiation.

Authors:  Kirsten Skarstad; Anders Løbner-Olesen
Journal:  EMBO J       Date:  2003-01-02       Impact factor: 11.598

4.  Identification of long intergenic repeat sequences associated with DNA methylation sites in Caulobacter crescentus and other alpha-proteobacteria.

Authors:  Swaine L Chen; Lucy Shapiro
Journal:  J Bacteriol       Date:  2003-08       Impact factor: 3.490

5.  Effect of site-specific methylation on DNA modification methyltransferases and restriction endonucleases.

Authors:  M McClelland; M Nelson
Journal:  Nucleic Acids Res       Date:  1992-05-11       Impact factor: 16.971

Review 6.  Organization of restriction-modification systems.

Authors:  G G Wilson
Journal:  Nucleic Acids Res       Date:  1991-05-25       Impact factor: 16.971

7.  Molecular cloning and characterization of the gene encoding the adenine methyltransferase M.CviRI from Chlorella virus XZ-6E.

Authors:  C Stefan; Y N Xia; J L Van Etten
Journal:  Nucleic Acids Res       Date:  1991-01-25       Impact factor: 16.971

8.  DNA mismatch correction by Very Short Patch repair may have altered the abundance of oligonucleotides in the E. coli genome.

Authors:  A S Bhagwat; M McClelland
Journal:  Nucleic Acids Res       Date:  1992-04-11       Impact factor: 16.971

9.  Overexpression of the wild-type gene coding for Escherichia coli DNA adenine methylase (dam).

Authors:  V U Nwosu
Journal:  Biochem J       Date:  1992-05-01       Impact factor: 3.857

10.  Site-specific methylation: effect on DNA modification methyltransferases and restriction endonucleases.

Authors:  M Nelson; M McClelland
Journal:  Nucleic Acids Res       Date:  1991-04-25       Impact factor: 16.971
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