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Literature DB >> 217004

DNA supercoiling by Xenopus laevis oocyte extracts: requirement for a nuclear factor.

M I Baldi, E Mattoccia, G P Tocchini-Valentini.

Abstract

A purified system is described for the introduction of negative supercoils into simian virus 40 DNA. The system consists of histones H2A, H2B, H3, and H4, DNA-relaxing enzyme, and a purified factor from Xenopus laevis stage 6 oocyte nuclei. The nuclei are prepared en masse by the technique of F. Scalenghe, M. Buscaglia, C. Steinheil, and M. Crippa [(1978) Chromosoma 60, 299-308]. The supercoiled simian virus 40 DNA prepared by this method is indistinguishable from simian virus 40 supercoiled DNA prepared from infected monkey cells.

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Year: 1978 PMID： 217004 PMCID： PMC336223 DOI： 10.1073/pnas.75.10.4873

Source DB: PubMed Journal: Proc Natl Acad Sci U S A ISSN： 0027-8424 Impact factor: 11.205

17 in total

1. Mechanism of action of nalidixic acid: purification of Escherichia coli nalA gene product and its relationship to DNA gyrase and a novel nicking-closing enzyme.

Authors: A Sugino; C L Peebles; K N Kreuzer; N R Cozzarelli
Journal: Proc Natl Acad Sci U S A Date: 1977-11 Impact factor: 11.205

DNA supercoiling by Xenopus laevis oocyte extracts: requirement for a nuclear factor.

1. Mechanism of action of nalidixic acid: purification of Escherichia coli nalA gene product and its relationship to DNA gyrase and a novel nicking-closing enzyme.

2. DNA gyrase: an enzyme that introduces superhelical turns into DNA.

3. DNA-relaxing activity and endonuclease activity in Xenopus laevis oocytes.

4. Nuclear localisation of an oocyte component required for the stability of injected DNA.

5. The synthesis and storage of histones during the oogenesis of Xenopus laevis.

6. Large scale isolation of nuclei and nucleoli from vitellogenic oocytes of Xenopus laevis.

7. Construction and characterization of new cloning vehicles. I. Ampicillin-resistant derivatives of the plasmid pMB9.

8. Nalidixic acid resistance: a second genetic character involved in DNA gyrase activity.

9. Micrococcus luteus DNA gyrase: active components and a model for its supercoiling of DNA.

10. Assembly of SV40 chromatin in a cell-free system from Xenopus eggs.

1. Replication and supercoiling of simian virus 40 DNA in cell extracts from human cells.

2. In vitro core particle and nucleosome assembly at physiological ionic strength.

3. Effects of ATP and inhibitory factors on the activity of vaccinia virus type I topoisomerase.