Literature DB >> 657930

Large scale isolation of nuclei and nucleoli from vitellogenic oocytes of Xenopus laevis.

F Scalenghe, M Buscaglia, C Steinheil, M Crippa.   

Abstract

A new method is described which allows the purification of large quantities of nuclei (germinal vesicles) from Xenopus laevis vitellogenic oocytes of different developmental stages. From the isolated nuclei, purified nucleoli were obtained. The isolated germinal vesicles are transcriptionally active showing endogenous RNA polymerase activity as well as a high level of activity with an exogenously added template.

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Year:  1978        PMID： 657930     DOI： 10.1007/BF00328531

Source DB:  PubMed          Journal:  Chromosoma        ISSN： 0009-5915            Impact factor:   4.316


  13 in total

1.  Defined nutrient medium for the in vitro maintenance of Xenopus laevis oocytes.

Authors:  J J Eppig; J N Dumont
Journal:  In Vitro       Date:  1976-06

2.  High-fidelity transcription of 5S DNA injected into Xenopus oocytes.

Authors:  D D Brown; J B Gurdon
Journal:  Proc Natl Acad Sci U S A       Date:  1977-05       Impact factor: 11.205

3.  Oogenesis in Xenopus laevis (Daudin). I. Stages of oocyte development in laboratory maintained animals.

Authors:  J N Dumont
Journal:  J Morphol       Date:  1972-02       Impact factor: 1.804

Review 4.  Specific gene amplification in oocytes. Oocyte nuclei contain extrachromosomal replicas of the genes for ribosomal RNA.

Authors:  D D Brown; I B Dawid
Journal:  Science       Date:  1968-04-19       Impact factor: 47.728

5.  Localization of amplified ribosomal DNA in the oocyte of Xenopus laevis.

Authors:  D Evans; M L Birnstiel
Journal:  Biochim Biophys Acta       Date:  1968-08-23

6.  Persistence in early amphibian embryos of informational RNA's from the lampbrush chromosome stage of oögenesis.

Authors:  M Crippa; E H Davidson; A E Mirsky
Journal:  Proc Natl Acad Sci U S A       Date:  1967-04       Impact factor: 11.205

7.  Genetic information in oocyte RNA.

Authors:  E H Davidson; B R Hough
Journal:  J Mol Biol       Date:  1971-03-28       Impact factor: 5.469

8.  Purified DNAs are transcribed after microinjection into Xenopus oocytes.

Authors:  J E Mertz; J B Gurdon
Journal:  Proc Natl Acad Sci U S A       Date:  1977-04       Impact factor: 11.205

9.  Coupled transcription-translation of DNA injected into Xenopus oocytes.

Authors:  E M De Robertis; J E Mertz
Journal:  Cell       Date:  1977-09       Impact factor: 41.582

10.  Transcription of xenopus tDNAmet1 and sea urchin histone DNA injected into the Xenopus oocyte nucleus.

Authors:  A Kressmann; S G Clarkson; J L Telford; M L Birnstiel
Journal:  Cold Spring Harb Symp Quant Biol       Date:  1978
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  21 in total

1.  A novel karyoskeletal protein: characterization of protein NO145, the major component of nucleolar cortical skeleton in Xenopus oocytes.

Authors:  S Kneissel; W W Franke; J G Gall; H Heid; S Reidenbach; M Schnölzer; H Spring; H Zentgraf; M S Schmidt-Zachmann
Journal:  Mol Biol Cell       Date:  2001-12       Impact factor: 4.138

2.  NO66, a highly conserved dual location protein in the nucleolus and in a special type of synchronously replicating chromatin.

Authors:  Jens Eilbracht; Michaela Reichenzeller; Michaela Hergt; Martina Schnölzer; Hans Heid; Michael Stöhr; Werner W Franke; Marion S Schmidt-Zachmann
Journal:  Mol Biol Cell       Date:  2004-01-23       Impact factor: 4.138

3.  DNA supercoiling by Xenopus laevis oocyte extracts: requirement for a nuclear factor.

Authors:  M I Baldi; E Mattoccia; G P Tocchini-Valentini
Journal:  Proc Natl Acad Sci U S A       Date:  1978-10       Impact factor: 11.205

4.  Neural-inducing activity of nuclei and nuclear fractions from Xenopus laevis embryos.

Authors:  Günther Bretzel; Hildegard Tiedemann
Journal:  Rouxs Arch Dev Biol       Date:  1986-02

5.  Tissue specific nuclear antigens in the germinal vesicle ofXenopus laevis oocytes.

Authors:  Christine Dreyer; Helen Singer; Peter Hausen; Ursula Müller; Elisabeth Siegel
Journal:  Wilehm Roux Arch Dev Biol       Date:  1981-07

6.  A novel helicase-type protein in the nucleolus: protein NOH61.

Authors:  R F Zirwes; J Eilbracht; S Kneissel; M S Schmidt-Zachmann
Journal:  Mol Biol Cell       Date:  2000-04       Impact factor: 4.138

7.  In vitro RNA synthesis in oocyte nuclei of the newt Notophthalmus.

Authors:  L D Schultz; B K Kay; J G Gall
Journal:  Chromosoma       Date:  1981       Impact factor: 4.316

8.  Symplekin, a constitutive protein of karyo- and cytoplasmic particles involved in mRNA biogenesis in Xenopus laevis oocytes.

Authors:  Ilse Hofmann; Martina Schnölzer; Isabelle Kaufmann; Werner W Franke
Journal:  Mol Biol Cell       Date:  2002-05       Impact factor: 4.138

9.  Immunological analysis of nuclear lamina proteins.

Authors:  R Stick; P Hausen
Journal:  Chromosoma       Date:  1980       Impact factor: 4.316

10.  The distribution of nucleoplasmin in early development and organogenesis of Xenopus laevis.

Authors:  D Wedlich; C Dreyer
Journal:  Cell Tissue Res       Date:  1988-11       Impact factor: 5.249
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