Literature DB >> 21622792

Comparison of genotypic and phylogenetic relationships of environmental Enterococcus isolates by BOX-PCR typing and 16S rRNA gene sequencing.

Bina S Nayak1, Brian Badgley, Valerie J Harwood.   

Abstract

Environmental Enterococcus spp. were compared by BOX-PCR genotyping and 16S rRNA gene sequencing to clarify the predictive relationship of BOX-PCR fingerprints to species designation. BOX-PCR and 16S rRNA gene relationships agreed for 77% of strains. BOX-PCR provided superior intraspecies discrimination but incorrectly identified some strains to the species level and divided some species into multiple groups.

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Year:  2011        PMID： 21622792      PMCID： PMC3147396          DOI： 10.1128/AEM.00130-11

Source DB:  PubMed          Journal:  Appl Environ Microbiol        ISSN： 0099-2240            Impact factor:   4.792


  35 in total

1.  Routine molecular identification of enterococci by gene-specific PCR and 16S ribosomal DNA sequencing.

Authors:  S Angeletti; G Lorino; G Gherardi; F Battistoni; M De Cesaris ; G Dicuonzo
Journal:  J Clin Microbiol       Date:  2001-02       Impact factor: 5.948

2.  Multilocus sequence typing scheme for Enterococcus faecalis reveals hospital-adapted genetic complexes in a background of high rates of recombination.

Authors:  Patricia Ruiz-Garbajosa; Marc J M Bonten; D Ashley Robinson; Janetta Top; Sreedhar R Nallapareddy; Carmen Torres; Teresa M Coque; Rafael Cantón; Fernando Baquero; Barbara E Murray; Rosa del Campo; Rob J L Willems
Journal:  J Clin Microbiol       Date:  2006-06       Impact factor: 5.948

3.  Comparison of 16S rRNA sequencing with conventional and commercial phenotypic techniques for identification of enterococci from the marine environment.

Authors:  D F Moore; M H Zhowandai; D M Ferguson; C McGee; J B Mott; J C Stewart
Journal:  J Appl Microbiol       Date:  2006-06       Impact factor: 3.772

4.  MEGA4: Molecular Evolutionary Genetics Analysis (MEGA) software version 4.0.

Authors:  Koichiro Tamura; Joel Dudley; Masatoshi Nei; Sudhir Kumar
Journal:  Mol Biol Evol       Date:  2007-05-07       Impact factor: 16.240

5.  rep-PCR fingerprinting and taxonomy based on the sequencing of the 16S rRNA gene of 54 elite commercial rhizobial strains.

Authors:  Daisy Rickli Binde; Pâmela Menna; Eliane Villamil Bangel; Fernando Gomes Barcellos; Mariangela Hungria
Journal:  Appl Microbiol Biotechnol       Date:  2009-03-17       Impact factor: 4.813

6.  Evaluation of the enterococci indicator in biosolids using culture-based and quantitative PCR assays.

Authors:  Emily Viau; Jordan Peccia
Journal:  Water Res       Date:  2009-09-11       Impact factor: 11.236

7.  Fidelity of bacterial source tracking: Escherichia coli vs Enterococcus spp and minimizing assignment of isolates from nonlibrary sources.

Authors:  W M Hassan; R D Ellender; S Y Wang
Journal:  J Appl Microbiol       Date:  2007-02       Impact factor: 3.772

8.  Evidence that the enterococcal polysaccharide antigen gene (epa) cluster is widespread in Enterococcus faecalis and influences resistance to phagocytic killing of E. faecalis.

Authors:  Fang Teng; Karen D Jacques-Palaz; George M Weinstock; Barbara E Murray
Journal:  Infect Immun       Date:  2002-04       Impact factor: 3.441

9.  Antimicrobial resistance and molecular epidemiology of vancomycin-resistant enterococci from North America and Europe: a report from the SENTRY antimicrobial surveillance program.

Authors:  Lalitagauri M Deshpande; Thomas R Fritsche; Gary J Moet; Douglas J Biedenbach; Ronald N Jones
Journal:  Diagn Microbiol Infect Dis       Date:  2007-03-26       Impact factor: 2.803

10.  Confirmation of putative stormwater impact on water quality at a Florida beach by microbial source tracking methods and structure of indicator organism populations.

Authors:  M J Brownell; V J Harwood; R C Kurz; S M McQuaig; J Lukasik; T M Scott
Journal:  Water Res       Date:  2007-06-01       Impact factor: 11.236
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  4 in total

Review 1.  Enterococci in the environment.

Authors:  Muruleedhara N Byappanahalli; Meredith B Nevers; Asja Korajkic; Zachery R Staley; Valerie J Harwood
Journal:  Microbiol Mol Biol Rev       Date:  2012-12       Impact factor: 11.056

2.  Development of a Rapid Identification Method for the Differentiation of Enterococcus Species Using a Species-Specific Multiplex PCR Based on Comparative Genomics.

Authors:  Jongbin Park; Gwi-Deuk Jin; Jae In Pak; Jihyun Won; Eun Bae Kim
Journal:  Curr Microbiol       Date:  2017-02-22       Impact factor: 2.188

3.  Vancomycin-Resistant Enterococci and Bacterial Community Structure following a Sewage Spill into an Aquatic Environment.

Authors:  Suzanne Young; Bina Nayak; Shan Sun; Brian D Badgley; Jason R Rohr; Valerie J Harwood
Journal:  Appl Environ Microbiol       Date:  2016-08-30       Impact factor: 4.792

4.  Development of quantitative PCR assays targeting the 16S rRNA genes of Enterococcus spp. and their application to the identification of enterococcus species in environmental samples.

Authors:  Hodon Ryu; Michael Henson; Michael Elk; Carlos Toledo-Hernandez; John Griffith; Denene Blackwood; Rachel Noble; Michèle Gourmelon; Susan Glassmeyer; Jorge W Santo Domingo
Journal:  Appl Environ Microbiol       Date:  2012-10-19       Impact factor: 4.792
  4 in total

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