Literature DB >> 21270233

Simultaneous detection of influenza A, influenza B, and respiratory syncytial viruses and subtyping of influenza A H3N2 virus and H1N1 (2009) virus by multiplex real-time PCR.

Yu Chen1, Dawei Cui, Shufa Zheng, Shigui Yang, Jia Tong, Dagan Yang, Jian Fan, Jie Zhang, Bin Lou, Xuefen Li, Xiaoling Zhuge, Bo Ye, Baode Chen, Weilin Mao, Yajun Tan, Genyun Xu, Zhenjin Chen, Nan Chen, Lanjuan Li.   

Abstract

A multiplex real-time PCR assay was developed to simultaneously detect and discriminate influenza A virus subtypes, including novel H1N1 (2009) and seasonal H3N2 virus, influenza B virus, and respiratory syncytial virus (RSV) in a single test tube, with detection sensitivity and specificity of 99% and 100%, respectively, for the four pathogens.

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Year:  2011        PMID： 21270233      PMCID： PMC3122825          DOI： 10.1128/JCM.02184-10

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


  13 in total

1.  Comparison of a multiplex reverse transcription-PCR-enzyme hybridization assay with conventional viral culture and immunofluorescence techniques for the detection of seven viral respiratory pathogens.

Authors:  L Liolios; A Jenney; D Spelman; T Kotsimbos; M Catton; S Wesselingh
Journal:  J Clin Microbiol       Date:  2001-08       Impact factor: 5.948

2.  Simultaneous detection of influenza viruses A and B using real-time quantitative PCR.

Authors:  L J van Elden; M Nijhuis; P Schipper; R Schuurman; A M van Loon
Journal:  J Clin Microbiol       Date:  2001-01       Impact factor: 5.948

3.  Real-time PCR to improve the diagnosis of respiratory syncytial virus infection.

Authors:  R Mentel; U Wegner; R Bruns; L Gürtler
Journal:  J Med Microbiol       Date:  2003-10       Impact factor: 2.472

4.  Swine influenza A (H1N1) infection in two children--Southern California, March-April 2009.

Authors: 
Journal:  MMWR Morb Mortal Wkly Rep       Date:  2009-04-24       Impact factor: 17.586

5.  One-step multiplex RT-PCR for detection and subtyping of swine influenza H1, H3, N1, N2 viruses in clinical samples using a dual priming oligonucleotide (DPO) system.

Authors:  C S Lee; B K Kang; D H Lee; S H Lyou; B K Park; S K Ann; K Jung; D S Song
Journal:  J Virol Methods       Date:  2008-05-19       Impact factor: 2.014

6.  Rapid multiplex reverse transcription-PCR typing of influenza A and B virus, and subtyping of influenza A virus into H1, 2, 3, 5, 7, 9, N1 (human), N1 (animal), N2, and N7, including typing of novel swine origin influenza A (H1N1) virus, during the 2009 outbreak in Milwaukee, Wisconsin.

Authors:  Jie He; Michael E Bose; Eric T Beck; Jiang Fan; Sagarika Tiwari; Jacob Metallo; Lisa A Jurgens; Sue C Kehl; Nathan Ledeboer; Swati Kumar; William Weisburg; Kelly J Henrickson
Journal:  J Clin Microbiol       Date:  2009-07-29       Impact factor: 5.948

7.  High-throughput, sensitive, and accurate multiplex PCR-microsphere flow cytometry system for large-scale comprehensive detection of respiratory viruses.

Authors:  Wai-Ming Lee; Kris Grindle; Tressa Pappas; David J Marshall; Michael J Moser; Edward L Beaty; Peter A Shult; James R Prudent; James E Gern
Journal:  J Clin Microbiol       Date:  2007-05-30       Impact factor: 5.948

8.  Development of a rapid automated influenza A, influenza B, and respiratory syncytial virus A/B multiplex real-time RT-PCR assay and its use during the 2009 H1N1 swine-origin influenza virus epidemic in Milwaukee, Wisconsin.

Authors:  Eric T Beck; Lisa A Jurgens; Sue C Kehl; Michael E Bose; Teresa Patitucci; Elizabeth LaGue; Patrick Darga; Kimberly Wilkinson; Lorraine M Witt; Jiang Fan; Jie He; Swati Kumar; Kelly J Henrickson
Journal:  J Mol Diagn       Date:  2009-12-03       Impact factor: 5.568

9.  Diagnostic system for rapid and sensitive differential detection of pathogens.

Authors:  Thomas Briese; Gustavo Palacios; Mark Kokoris; Omar Jabado; Zhiqiang Liu; Neil Renwick; Vishal Kapoor; Inmaculada Casas; Francisco Pozo; Ron Limberger; Pilar Perez-Brena; Jingyue Ju; W Ian Lipkin
Journal:  Emerg Infect Dis       Date:  2005-02       Impact factor: 6.883

10.  Simultaneous detection of fourteen respiratory viruses in clinical specimens by two multiplex reverse transcription nested-PCR assays.

Authors:  M T Coiras; J C Aguilar; M L García; I Casas; P Pérez-Breña
Journal:  J Med Virol       Date:  2004-03       Impact factor: 2.327
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  22 in total

1.  Evaluation of Simplexa Flu A/B & RSV for direct detection of influenza viruses (A and B) and respiratory syncytial virus in patient clinical samples.

Authors:  Musa Hindiyeh; Liat Kolet; Tal Meningher; Merav Weil; Ella Mendelson; Michal Mandelboim
Journal:  J Clin Microbiol       Date:  2013-05-08       Impact factor: 5.948

2.  Development of one-step real-time PCR assay for titrating trivalent live attenuated influenza vaccines.

Authors:  Yang Zang; Dongchuan Du; Peng Ge; Yongqing Xu; Xintao Liu; Yan Zhang; Weiheng Su; Irina Kiseleva; Larisa Rudenko; Fei Xu; Wei Kong; Chunlai Jiang
Journal:  Hum Vaccin Immunother       Date:  2014       Impact factor: 3.452

Review 3.  Virological and Immunological Outcomes of Coinfections.

Authors:  Naveen Kumar; Shalini Sharma; Sanjay Barua; Bhupendra N Tripathi; Barry T Rouse
Journal:  Clin Microbiol Rev       Date:  2018-07-05       Impact factor: 26.132

4.  The development of a GeXP-based multiplex reverse transcription-PCR assay for simultaneous detection of sixteen human respiratory virus types/subtypes.

Authors:  Jin Li; Nai-Ying Mao; Chen Zhang; Meng-Jie Yang; Miao Wang; Wen-Bo Xu; Xue-Jun Ma
Journal:  BMC Infect Dis       Date:  2012-08-14       Impact factor: 3.090

5.  Public Health Responses to Reemergence of Animal Rabies, Taiwan, July 16-December 28, 2013.

Authors:  Angela Song-En Huang; Wan-Chin Chen; Wan-Ting Huang; Shih-Tse Huang; Yi-Chun Lo; Sung-Hsi Wei; Hung-Wei Kuo; Pei-Chun Chan; Min-Nan Hung; Yu-Lun Liu; Jung-Jung Mu; Jyh-Yuan Yang; Ding-Ping Liu; Jih-Haw Chou; Jen-Hsiang Chuang; Feng-Yee Chang
Journal:  PLoS One       Date:  2015-07-10       Impact factor: 3.240

6.  Detection of a novel avian influenza A (H7N9) virus in humans by multiplex one-step real-time RT-PCR assay.

Authors:  Jian Fan; David Cui; Siuying Lau; Guoliang Xie; Xichao Guo; Shufa Zheng; Xiaofeng Huang; Shigui Yang; Xianzhi Yang; Zhaoxia Huo; Fei Yu; Jianzhou Lou; Li Tian; Xuefen Li; Yuejiao Dong; Qiaoyun Zhu; Yu Chen
Journal:  BMC Infect Dis       Date:  2014-10-08       Impact factor: 3.090

7.  Avian influenza viruses that cause highly virulent infections in humans exhibit distinct replicative properties in contrast to human H1N1 viruses.

Authors:  Philippe F Simon; Marc-Antoine de La Vega; Éric Paradis; Emelissa Mendoza; Kevin M Coombs; Darwyn Kobasa; Catherine A A Beauchemin
Journal:  Sci Rep       Date:  2016-04-15       Impact factor: 4.379

8.  Detecting respiratory viral RNA using expanded genetic alphabets and self-avoiding DNA.

Authors:  Lyudmyla G Glushakova; Nidhi Sharma; Shuichi Hoshika; Andrea C Bradley; Kevin M Bradley; Zunyi Yang; Steven A Benner
Journal:  Anal Biochem       Date:  2015-08-21       Impact factor: 3.365

9.  Evaluation of custom multiplex real - time RT - PCR in comparison to fast - track diagnostics respiratory 21 pathogens kit for detection of multiple respiratory viruses.

Authors:  Bharti Malhotra; M Anjaneya Swamy; P V Janardhan Reddy; Neeraj Kumar; Jitendra Kumar Tiwari
Journal:  Virol J       Date:  2016-06-06       Impact factor: 4.099

10.  Giant Magnetoresistance-based Biosensor for Detection of Influenza A Virus.

Authors:  Venkatramana D Krishna; Kai Wu; Andres M Perez; Jian-Ping Wang
Journal:  Front Microbiol       Date:  2016-03-29       Impact factor: 5.640
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