Literature DB >> 23658256

Evaluation of Simplexa Flu A/B & RSV for direct detection of influenza viruses (A and B) and respiratory syncytial virus in patient clinical samples.

Musa Hindiyeh1, Liat Kolet, Tal Meningher, Merav Weil, Ella Mendelson, Michal Mandelboim.   

Abstract

We evaluated the performance of the Simplexa Flu A/B & RSV kit on 170 prospective respiratory samples using a modified protocol, supplied by the manufacturer, that eliminates the RNA extraction step. Overall, compared against our laboratory-developed assay, the assay's sensitivity, specificity, and positive and negative predictive values were 95.1%, 99.6%, 98.7%, and 98.6%, respectively.

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Year:  2013        PMID： 23658256      PMCID： PMC3697722          DOI： 10.1128/JCM.00286-13

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


  12 in total

1.  Simultaneous detection of influenza A, influenza B, and respiratory syncytial viruses and subtyping of influenza A H3N2 virus and H1N1 (2009) virus by multiplex real-time PCR.

Authors:  Yu Chen; Dawei Cui; Shufa Zheng; Shigui Yang; Jia Tong; Dagan Yang; Jian Fan; Jie Zhang; Bin Lou; Xuefen Li; Xiaoling Zhuge; Bo Ye; Baode Chen; Weilin Mao; Yajun Tan; Genyun Xu; Zhenjin Chen; Nan Chen; Lanjuan Li
Journal:  J Clin Microbiol       Date:  2011-01-26       Impact factor: 5.948

Review 2.  Nucleic acid amplification-based diagnosis of respiratory virus infections.

Authors:  James B Mahony
Journal:  Expert Rev Anti Infect Ther       Date:  2010-11       Impact factor: 5.091

3.  Comparison of the GenMark Diagnostics eSensor respiratory viral panel to real-time PCR for detection of respiratory viruses in children.

Authors:  Virginia M Pierce; Richard L Hodinka
Journal:  J Clin Microbiol       Date:  2012-08-08       Impact factor: 5.948

Review 4.  Molecular diagnosis of respiratory virus infections.

Authors:  James B Mahony; Astrid Petrich; Marek Smieja
Journal:  Crit Rev Clin Lab Sci       Date:  2011 Sep-Dec       Impact factor: 6.250

5.  Evaluation of the Prodesse Hexaplex multiplex PCR assay for direct detection of seven respiratory viruses in clinical specimens.

Authors:  M Hindiyeh; D R Hillyard; K C Carroll
Journal:  Am J Clin Pathol       Date:  2001-08       Impact factor: 2.493

6.  Comparative evaluation of the Nanosphere Verigene RV+ assay and the Simplexa Flu A/B & RSV kit for detection of influenza and respiratory syncytial viruses.

Authors:  Kevin Alby; Elena B Popowitch; Melissa B Miller
Journal:  J Clin Microbiol       Date:  2012-11-14       Impact factor: 5.948

7.  Detection of influenza A(H1N1)v virus by real-time RT-PCR.

Authors:  M Panning; M Eickmann; O Landt; M Monazahian; S Olschläger; S Baumgarte; U Reischl; J J Wenzel; H H Niller; S Günther; B Hollmann; D Huzly; J F Drexler; A Helmer; S Becker; B Matz; Am Eis-Hübinger; C Drosten
Journal:  Euro Surveill       Date:  2009-09-10

8.  Simultaneous detection, subgrouping, and quantitation of respiratory syncytial virus A and B by real-time PCR.

Authors:  Aizhong Hu; Melissa Colella; John S Tam; Ruth Rappaport; Sheau-Mei Cheng
Journal:  J Clin Microbiol       Date:  2003-01       Impact factor: 5.948

9.  Diagnosis of respiratory syncytial virus infection.

Authors:  Therese Popow-Kraupp; Judith H Aberle
Journal:  Open Microbiol J       Date:  2011-12-30

10.  Evaluation of multiple test methods for the detection of the novel 2009 influenza A (H1N1) during the New York City outbreak.

Authors:  Christine C Ginocchio; Frank Zhang; Ryhana Manji; Suman Arora; Mark Bornfreund; Leon Falk; Madhavi Lotlikar; Margaret Kowerska; George Becker; Diamanto Korologos; Marcella de Geronimo; James M Crawford
Journal:  J Clin Virol       Date:  2009-06-16       Impact factor: 3.168
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  9 in total

1.  Comparison of Simplexa Flu A/B & RSV PCR with cytospin-immunofluorescence and laboratory-developed TaqMan PCR in predominantly adult hospitalized patients.

Authors:  Marie L Landry; David Ferguson
Journal:  J Clin Microbiol       Date:  2014-05-21       Impact factor: 5.948

2.  Comparison of the Simplexa FluA/B & RSV direct assay and laboratory-developed real-time PCR assays for detection of respiratory virus.

Authors:  Mitchell W Woodberry; Rohit Shankar; Anne Cent; Keith R Jerome; Jane Kuypers
Journal:  J Clin Microbiol       Date:  2013-09-18       Impact factor: 5.948

3.  Comparison of Six Sample-to-Answer Influenza A/B and Respiratory Syncytial Virus Nucleic Acid Amplification Assays Using Respiratory Specimens from Children.

Authors:  Dithi Banerjee; Neena Kanwar; Ferdaus Hassan; Cynthia Essmyer; Rangaraj Selvarangan
Journal:  J Clin Microbiol       Date:  2018-10-25       Impact factor: 5.948

4.  Comparison of the new fully automated extraction platform eMAG to the MagNA PURE 96 and the well-established easyMAG for detection of common human respiratory viruses.

Authors:  Musa Hindiyeh; Orna Mor; Rakefet Pando; Batya Mannasse; Areej Kabat; Hadar Assraf-Zarfati; Ella Mendelson; Danit Sofer; Michal Mandelboim
Journal:  PLoS One       Date:  2019-02-19       Impact factor: 3.240

Review 5.  Rapid diagnosis of influenza: state of the art.

Authors:  David R Peaper; Marie L Landry
Journal:  Clin Lab Med       Date:  2014-06       Impact factor: 1.935

Review 6.  The new frontier of diagnostics: Molecular assays and their role in infection prevention and control.

Authors:  Sanchita Das; Dena R Shibib; Michael O Vernon
Journal:  Am J Infect Control       Date:  2017-02-01       Impact factor: 2.918

7.  Performance evaluation of direct fluorescent antibody, Focus Diagnostics Simplexa™ Flu A/B & RSV and multi-parameter customized respiratory Taqman® array card in immunocompromised patients.

Authors:  Deborah Steensels; Marijke Reynders; Patrick Descheemaeker; Martin D Curran; Frédérique Jacobs; Olivier Denis; Marie-Luce Delforge; Isabel Montesinos
Journal:  J Virol Methods       Date:  2017-03-30       Impact factor: 2.014

8.  Integrated Trinity Test With RPA-CRISPR/Cas12a-Fluorescence for Real-Time Detection of Respiratory Syncytial Virus A or B.

Authors:  Ling Gong; Xiaowen Wang; Zhu Li; Guichuan Huang; Wei Zhang; Jin Nie; Chunyan Wu; Daishun Liu
Journal:  Front Microbiol       Date:  2022-03-31       Impact factor: 5.640

9.  Respiratory syncytial virus infection-associated hospitalization in adults: a retrospective cohort study.

Authors:  Cheryl Volling; Kazi Hassan; Tony Mazzulli; Karen Green; Ahmed Al-Den; Paul Hunter; Rupi Mangat; John Ng; Allison McGeer
Journal:  BMC Infect Dis       Date:  2014-12-13       Impact factor: 3.090
  9 in total

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