Literature DB >> 12972584

Real-time PCR to improve the diagnosis of respiratory syncytial virus infection.

R Mentel1, U Wegner1, R Bruns1, L Gürtler1.   

Abstract

Respiratory syncytial virus (RSV) is one of the most important virus respiratory pathogens in infants and young children. A rapid and sensitive diagnosis is essential to focus any outbreak due to this virus. A real-time RT-PCR method was designed using a primer/probe pair from the F gene. Simultaneously with nested RT-PCR and antigen ELISA, 71 consecutive specimens from hospitalized children with clinical symptoms of acute respiratory distress were evaluated to confirm the incidence of RSV infection. RSV was detected in 25 (35.2 %) specimens by real-time RT-PCR and in 19 (26.7 %) by nested RT-PCR. The assay was specific for RSV. The procedure offers a rapid and sensitive alternative to conventional RT-PCR. Closed-tube detection eliminates the risk of contamination.

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Year:  2003        PMID： 12972584     DOI： 10.1099/jmm.0.05290-0

Source DB:  PubMed          Journal:  J Med Microbiol        ISSN： 0022-2615            Impact factor:   2.472


  36 in total

1.  Simultaneous detection of influenza A, influenza B, and respiratory syncytial viruses and subtyping of influenza A H3N2 virus and H1N1 (2009) virus by multiplex real-time PCR.

Authors:  Yu Chen; Dawei Cui; Shufa Zheng; Shigui Yang; Jia Tong; Dagan Yang; Jian Fan; Jie Zhang; Bin Lou; Xuefen Li; Xiaoling Zhuge; Bo Ye; Baode Chen; Weilin Mao; Yajun Tan; Genyun Xu; Zhenjin Chen; Nan Chen; Lanjuan Li
Journal:  J Clin Microbiol       Date:  2011-01-26       Impact factor: 5.948

Review 2.  Real-time PCR in clinical microbiology: applications for routine laboratory testing.

Authors:  M J Espy; J R Uhl; L M Sloan; S P Buckwalter; M F Jones; E A Vetter; J D C Yao; N L Wengenack; J E Rosenblatt; F R Cockerill; T F Smith
Journal:  Clin Microbiol Rev       Date:  2006-01       Impact factor: 26.132

3.  Two-Step versus One-Step RNA-to-CT 2-Step and One-Step RNA-to-CT 1-Step: validity, sensitivity, and efficiency.

Authors:  Nasser Al-Shanti; Amarjit Saini; Claire E Stewart
Journal:  J Biomol Tech       Date:  2009-07

4.  RSV associated hospitalizations in children in Karachi, Pakistan: Implications for vaccine prevention strategies.

Authors:  Asad Ali; Mohammad Tahir Yousafzai; Rabbia Waris; Fatima Jafri; Fatima Aziz; Imran Naeem Abbasi; Anita Zaidi
Journal:  J Med Virol       Date:  2017-02-24       Impact factor: 2.327

Review 5.  Detection of respiratory viruses by molecular methods.

Authors:  James B Mahony
Journal:  Clin Microbiol Rev       Date:  2008-10       Impact factor: 26.132

6.  Molecular and clinical characteristics of respiratory syncytial virus infections in hospitalized children.

Authors:  R Mentel; U Ilgert; U Wegner; K Zimmerman; R Bruns; L Gürtler
Journal:  Med Microbiol Immunol       Date:  2005-01       Impact factor: 3.402

7.  Comprehensive detection of causative pathogens using real-time PCR to diagnose pediatric community-acquired pneumonia.

Authors:  Keiko Hamano-Hasegawa; Miyuki Morozumi; Eiichi Nakayama; Naoko Chiba; Somay Y Murayama; Reiko Takayanagi; Satoshi Iwata; Keisuke Sunakawa; Kimiko Ubukata
Journal:  J Infect Chemother       Date:  2008-12-17       Impact factor: 2.211

8.  Venezuelan equine encephalitis virus replicon particles encoding respiratory syncytial virus surface glycoproteins induce protective mucosal responses in mice and cotton rats.

Authors:  Hoyin Mok; Sujin Lee; Thomas J Utley; Bryan E Shepherd; Vasiliy V Polosukhin; Martha L Collier; Nancy L Davis; Robert E Johnston; James E Crowe
Journal:  J Virol       Date:  2007-10-10       Impact factor: 5.103

9.  High-throughput screening normalized to biological response: application to antiviral drug discovery.

Authors:  Dhara A Patel; Anand C Patel; William C Nolan; Guangming Huang; Arthur G Romero; Nichole Charlton; Eugene Agapov; Yong Zhang; Michael J Holtzman
Journal:  J Biomol Screen       Date:  2013-07-16

10.  A chimeric A2 strain of respiratory syncytial virus (RSV) with the fusion protein of RSV strain line 19 exhibits enhanced viral load, mucus, and airway dysfunction.

Authors:  Martin L Moore; Michael H Chi; Cindy Luongo; Nicholas W Lukacs; Vasiliy V Polosukhin; Matthew M Huckabee; Dawn C Newcomb; Ursula J Buchholz; James E Crowe; Kasia Goleniewska; John V Williams; Peter L Collins; R Stokes Peebles
Journal:  J Virol       Date:  2009-02-11       Impact factor: 5.103
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