Literature DB >> 21258339

Micropilot: automation of fluorescence microscopy-based imaging for systems biology.

Christian Conrad1, Annelie Wünsche, Tze Heng Tan, Jutta Bulkescher, Frank Sieckmann, Fatima Verissimo, Arthur Edelstein, Thomas Walter, Urban Liebel, Rainer Pepperkok, Jan Ellenberg.   

Abstract

Quantitative microscopy relies on imaging of large cell numbers but is often hampered by time-consuming manual selection of specific cells. The 'Micropilot' software automatically detects cells of interest and launches complex imaging experiments including three-dimensional multicolor time-lapse or fluorescence recovery after photobleaching in live cells. In three independent experimental setups this allowed us to statistically analyze biological processes in detail and is thus a powerful tool for systems biology.

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Year:  2011        PMID： 21258339      PMCID： PMC3086017          DOI： 10.1038/nmeth.1558

Source DB:  PubMed          Journal:  Nat Methods        ISSN： 1548-7091            Impact factor:   28.547


  15 in total

1.  CellCognition: time-resolved phenotype annotation in high-throughput live cell imaging.

Authors:  Michael Held; Michael H A Schmitz; Bernd Fischer; Thomas Walter; Beate Neumann; Michael H Olma; Matthias Peter; Jan Ellenberg; Daniel W Gerlich
Journal:  Nat Methods       Date:  2010-08-08       Impact factor: 28.547

2.  High-throughput RNAi screening by time-lapse imaging of live human cells.

Authors:  Beate Neumann; Michael Held; Urban Liebel; Holger Erfle; Phill Rogers; Rainer Pepperkok; Jan Ellenberg
Journal:  Nat Methods       Date:  2006-05       Impact factor: 28.547

3.  CellClassifier: supervised learning of cellular phenotypes.

Authors:  Pauli Rämö; Raphael Sacher; Berend Snijder; Boris Begemann; Lucas Pelkmans
Journal:  Bioinformatics       Date:  2009-09-02       Impact factor: 6.937

4.  Phenotypic profiling of the human genome by time-lapse microscopy reveals cell division genes.

Authors:  Beate Neumann; Thomas Walter; Jean-Karim Hériché; Jutta Bulkescher; Holger Erfle; Christian Conrad; Phill Rogers; Ina Poser; Michael Held; Urban Liebel; Cihan Cetin; Frank Sieckmann; Gregoire Pau; Rolf Kabbe; Annelie Wünsche; Venkata Satagopam; Michael H A Schmitz; Catherine Chapuis; Daniel W Gerlich; Reinhard Schneider; Roland Eils; Wolfgang Huber; Jan-Michael Peters; Anthony A Hyman; Richard Durbin; Rainer Pepperkok; Jan Ellenberg
Journal:  Nature       Date:  2010-04-01       Impact factor: 49.962

5.  Systematic analysis of human protein complexes identifies chromosome segregation proteins.

Authors:  James R A Hutchins; Yusuke Toyoda; Björn Hegemann; Ina Poser; Jean-Karim Hériché; Martina M Sykora; Martina Augsburg; Otto Hudecz; Bettina A Buschhorn; Jutta Bulkescher; Christian Conrad; David Comartin; Alexander Schleiffer; Mihail Sarov; Andrei Pozniakovsky; Mikolaj Michal Slabicki; Siegfried Schloissnig; Ines Steinmacher; Marit Leuschner; Andrea Ssykor; Steffen Lawo; Laurence Pelletier; Holger Stark; Kim Nasmyth; Jan Ellenberg; Richard Durbin; Frank Buchholz; Karl Mechtler; Anthony A Hyman; Jan-Michael Peters
Journal:  Science       Date:  2010-04-01       Impact factor: 47.728

Review 6.  Automated microscopy for high-content RNAi screening.

Authors:  Christian Conrad; Daniel W Gerlich
Journal:  J Cell Biol       Date:  2010-02-22       Impact factor: 10.539

7.  EBImage--an R package for image processing with applications to cellular phenotypes.

Authors:  Grégoire Pau; Florian Fuchs; Oleg Sklyar; Michael Boutros; Wolfgang Huber
Journal:  Bioinformatics       Date:  2010-04-01       Impact factor: 6.937

8.  Multiscale analysis of dynamics and interactions of heterochromatin protein 1 by fluorescence fluctuation microscopy.

Authors:  Katharina P Müller; Fabian Erdel; Maïwen Caudron-Herger; Caroline Marth; Barna D Fodor; Mario Richter; Manuela Scaranaro; Joël Beaudouin; Malte Wachsmuth; Karsten Rippe
Journal:  Biophys J       Date:  2009-12-02       Impact factor: 4.033

9.  CENP-E function at kinetochores is essential for chromosome alignment.

Authors:  B T Schaar; G K Chan; P Maddox; E D Salmon; T J Yen
Journal:  J Cell Biol       Date:  1997-12-15       Impact factor: 10.539

10.  Building a spindle of the correct length in human cells requires the interaction between TPX2 and Aurora A.

Authors:  Alexander W Bird; Anthony A Hyman
Journal:  J Cell Biol       Date:  2008-07-28       Impact factor: 10.539
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  42 in total

1.  Semantic integration of physiology phenotypes with an application to the Cellular Phenotype Ontology.

Authors:  Robert Hoehndorf; Midori A Harris; Heinrich Herre; Gabriella Rustici; Georgios V Gkoutos
Journal:  Bioinformatics       Date:  2012-04-26       Impact factor: 6.937

2.  CellSeT: novel software to extract and analyze structured networks of plant cells from confocal images.

Authors:  Michael P Pound; Andrew P French; Darren M Wells; Malcolm J Bennett; Tony P Pridmore
Journal:  Plant Cell       Date:  2012-04-03       Impact factor: 11.277

3.  Unsupervised modeling of cell morphology dynamics for time-lapse microscopy.

Authors:  Qing Zhong; Alberto Giovanni Busetto; Juan P Fededa; Joachim M Buhmann; Daniel W Gerlich
Journal:  Nat Methods       Date:  2012-05-27       Impact factor: 28.547

4.  The smart and gentle microscope.

Authors:  Nico Scherf; Jan Huisken
Journal:  Nat Biotechnol       Date:  2015-08       Impact factor: 54.908

Review 5.  Molecular diffusion and binding analyzed with FRAP.

Authors:  Malte Wachsmuth
Journal:  Protoplasma       Date:  2014-01-04       Impact factor: 3.356

6.  High-throughput fluorescence correlation spectroscopy enables analysis of proteome dynamics in living cells.

Authors:  Malte Wachsmuth; Christian Conrad; Jutta Bulkescher; Birgit Koch; Robert Mahen; Mayumi Isokane; Rainer Pepperkok; Jan Ellenberg
Journal:  Nat Biotechnol       Date:  2015-03-16       Impact factor: 54.908

7.  Let there be light-at the right place.

Authors:  Lars Hufnagel; Rainer Pepperkok
Journal:  Nat Biotechnol       Date:  2016-12-07       Impact factor: 54.908

8.  Quantitative mapping of fluorescently tagged cellular proteins using FCS-calibrated four-dimensional imaging.

Authors:  Antonio Z Politi; Yin Cai; Nike Walther; M Julius Hossain; Birgit Koch; Malte Wachsmuth; Jan Ellenberg
Journal:  Nat Protoc       Date:  2018-05-24       Impact factor: 13.491

9.  Microscope-based imaging platform for large-scale analysis of oral biofilms.

Authors:  L Karygianni; M Follo; E Hellwig; D Burghardt; M Wolkewitz; A Anderson; A Al-Ahmad
Journal:  Appl Environ Microbiol       Date:  2012-10-05       Impact factor: 4.792

10.  Frapid: achieving full automation of FRAP for chemical probe validation.

Authors:  Clarence Yapp; Catherine Rogers; Pavel Savitsky; Martin Philpott; Susanne Müller
Journal:  Biomed Opt Express       Date:  2016-01-11       Impact factor: 3.732
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