Literature DB >> 20935651

Chemical synthesis of the mouse mitochondrial genome.

Daniel G Gibson1, Hamilton O Smith, Clyde A Hutchison, J Craig Venter, Chuck Merryman.   

Abstract

We describe a one-step, isothermal assembly method for synthesizing DNA molecules from overlapping oligonucleotides. The method cycles between in vitro recombination and amplification until the desired length is reached. As a demonstration of its simplicity and robustness, we synthesized the entire 16.3-kilobase mouse mitochondrial genome from 600 overlapping 60-mers.

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Year:  2010        PMID： 20935651     DOI： 10.1038/nmeth.1515

Source DB:  PubMed          Journal:  Nat Methods        ISSN： 1548-7091            Impact factor:   28.547


  15 in total

Review 1.  Automation for genomics, part one: preparation for sequencing.

Authors:  D Meldrum
Journal:  Genome Res       Date:  2000-08       Impact factor: 9.043

Review 2.  Mitochondrial DNA mutations in human disease.

Authors:  Robert W Taylor; Doug M Turnbull
Journal:  Nat Rev Genet       Date:  2005-05       Impact factor: 53.242

Review 3.  Chemical gene synthesis: strategies, softwares, error corrections, and applications.

Authors:  Ai-Sheng Xiong; Ri-He Peng; Jing Zhuang; Feng Gao; Yi Li; Zong-Ming Cheng; Quan-Hong Yao
Journal:  FEMS Microbiol Rev       Date:  2008-04-02       Impact factor: 16.408

4.  Total synthesis of the gene for an alanine transfer ribonucleic acid from yeast.

Authors:  K L Agarwal; H Büchi; M H Caruthers; N Gupta; H G Khorana; K Kleppe; A Kumar; E Ohtsuka; U L Rajbhandary; J H Van de Sande; V Sgaramella; H Weber; T Yamada
Journal:  Nature       Date:  1970-07-04       Impact factor: 49.962

5.  Total synthesis of long DNA sequences: synthesis of a contiguous 32-kb polyketide synthase gene cluster.

Authors:  Sarah J Kodumal; Kedar G Patel; Ralph Reid; Hugo G Menzella; Mark Welch; Daniel V Santi
Journal:  Proc Natl Acad Sci U S A       Date:  2004-10-20       Impact factor: 11.205

6.  A microfluidic oligonucleotide synthesizer.

Authors:  Cheng-Chung Lee; Thomas M Snyder; Stephen R Quake
Journal:  Nucleic Acids Res       Date:  2010-02-21       Impact factor: 16.971

Review 7.  Synthetic biology: applications come of age.

Authors:  Ahmad S Khalil; James J Collins
Journal:  Nat Rev Genet       Date:  2010-05       Impact factor: 53.242

8.  Complete chemical synthesis, assembly, and cloning of a Mycoplasma genitalium genome.

Authors:  Daniel G Gibson; Gwynedd A Benders; Cynthia Andrews-Pfannkoch; Evgeniya A Denisova; Holly Baden-Tillson; Jayshree Zaveri; Timothy B Stockwell; Anushka Brownley; David W Thomas; Mikkel A Algire; Chuck Merryman; Lei Young; Vladimir N Noskov; John I Glass; J Craig Venter; Clyde A Hutchison; Hamilton O Smith
Journal:  Science       Date:  2008-01-24       Impact factor: 47.728

9.  Bottom-up genome assembly using the Bacillus subtilis genome vector.

Authors:  Mitsuhiro Itaya; Kyoko Fujita; Azusa Kuroki; Kenji Tsuge
Journal:  Nat Methods       Date:  2007-12-09       Impact factor: 28.547

10.  Design parameters to control synthetic gene expression in Escherichia coli.

Authors:  Mark Welch; Sridhar Govindarajan; Jon E Ness; Alan Villalobos; Austin Gurney; Jeremy Minshull; Claes Gustafsson
Journal:  PLoS One       Date:  2009-09-14       Impact factor: 3.240
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  119 in total

1.  New technologies for 21st century plant science.

Authors:  David W Ehrhardt; Wolf B Frommer
Journal:  Plant Cell       Date:  2012-02-24       Impact factor: 11.277

2.  DNA construction: homemade or ordered out?

Authors:  Peter A Carr
Journal:  Nat Methods       Date:  2010-11       Impact factor: 28.547

3.  Megabases for kilodollars.

Authors:  Mikkel Algire; Radha Krishnakumar; Chuck Merryman
Journal:  Nat Biotechnol       Date:  2010-12       Impact factor: 54.908

4.  High-throughput CRISPR Vector Construction and Characterization of DNA Modifications by Generation of Tomato Hairy Roots.

Authors:  Thomas B Jacobs; Gregory B Martin
Journal:  J Vis Exp       Date:  2016-04-30       Impact factor: 1.355

5.  Synthetic genomes: The next step for the synthetic genome.

Authors:  Monya Baker
Journal:  Nature       Date:  2011-05-19       Impact factor: 49.962

6.  Runx1t1 regulates the neuronal differentiation of radial glial cells from the rat hippocampus.

Authors:  Zou Linqing; Jin Guohua; Li Haoming; Tao Xuelei; Qin Jianbing; Tian Meiling
Journal:  Stem Cells Transl Med       Date:  2014-12-03       Impact factor: 6.940

Review 7.  Bricks and blueprints: methods and standards for DNA assembly.

Authors:  Arturo Casini; Marko Storch; Geoffrey S Baldwin; Tom Ellis
Journal:  Nat Rev Mol Cell Biol       Date:  2015-06-17       Impact factor: 94.444

8.  Phosphodeoxyribosyltransferases, designed enzymes for deoxyribonucleotides synthesis.

Authors:  Pierre Alexandre Kaminski; Gilles Labesse
Journal:  J Biol Chem       Date:  2013-01-16       Impact factor: 5.157

Review 9.  Synthetic biology in mammalian cells: next generation research tools and therapeutics.

Authors:  Florian Lienert; Jason J Lohmueller; Abhishek Garg; Pamela A Silver
Journal:  Nat Rev Mol Cell Biol       Date:  2014-01-17       Impact factor: 94.444

10.  PPE37 Is Essential for Mycobacterium tuberculosis Heme-Iron Acquisition (HIA), and a Defective PPE37 in Mycobacterium bovis BCG Prevents HIA.

Authors:  Michael V Tullius; Susana Nava; Marcus A Horwitz
Journal:  Infect Immun       Date:  2019-01-24       Impact factor: 3.441
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