| Literature DB >> 20670891 |
Rui Fang1, Andrew J Barbera, Yufei Xu, Michael Rutenberg, Thiago Leonor, Qing Bi, Fei Lan, Pinchao Mei, Guo-Cheng Yuan, Christine Lian, Junmin Peng, Dongmei Cheng, Guangchao Sui, Ursula B Kaiser, Yang Shi, Yujiang Geno Shi.
Abstract
Dynamic histone H3K4 methylation is an important epigenetic component of transcriptional regulation. However, most of our current understanding of this histone mark is confined to the regulation of transcriptional initiation. We now show that human LSD2/KDM1b/AOF1, the human homolog of LSD1, is an H3K4me1/2 demethylase that specifically regulates histone H3K4 methylation within intragenic regions of its target genes. Genome-wide mapping reveals that LSD2 associates predominantly with the gene bodies of actively transcribed genes, but is markedly absent from promoters. Depletion of endogenous LSD2 results in an increase of H3K4me2 as well as a decrease of H3K9me2 at LSD2-binding sites and a consequent dysregulation of target gene transcription. Furthermore, characterization of the LSD2 complex reveals that LSD2 forms active complexes with euchromatic histone methyltransferases G9a and NSD3 as well as cellular factors involved in transcription elongation. These data provide a possible molecular mechanism linking LSD2 to transcriptional regulation after initiation. Copyright 2010 Elsevier Inc. All rights reserved.Entities:
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Year: 2010 PMID： 20670891 PMCID： PMC3518444 DOI： 10.1016/j.molcel.2010.07.008
Source DB: PubMed Journal: Mol Cell ISSN： 1097-2765 Impact factor: 17.970