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Literature DB >> 204912

Interaction of the DNA untwisting enzyme with the SV40 nucleoprotein complex.

Abstract

The SV40 nucleoprotein complex which was isolated from infected CV-1 cells did not possess an active DNA untwisting enzyme. The superhelix density of the DNA in the chromatin complex was unchanged after treatment with purified rat liver DNA untwisting enzyme. However, in the presence of ethidium bromide (1 microgram/ml) the superhelix density was changed. Moreover, the nicked intermediate in the DNA untwisting reaction could be detected using the chromatin DNA as a substrate. These results show that the DNA in the SV40 chromatin which is accessible to the DNA untwisting enzyme is under no topological strain.

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Year: 1978 PMID： 204912 PMCID： PMC342007 DOI： 10.1093/nar/5.2.623

Source DB: PubMed Journal: Nucleic Acids Res ISSN： 0305-1048 Impact factor: 16.971

33 in total

1. Folding of the DNA double helix in chromatin-like structures from simian virus 40.

Authors: J E Germond; B Hirt; P Oudet; M Gross-Bellark; P Chambon
Journal: Proc Natl Acad Sci U S A Date: 1975-05 Impact factor: 11.205

2. Characterization of purified DNA-relaxing enzyme from human tissue culture cells.

Authors: W Keller
Journal: Proc Natl Acad Sci U S A Date: 1975-07 Impact factor: 11.205

3. Priming of superhelical SV40 DNA by Escherichia coli RNA polymerase for in vitro DNA synthesis.

Authors: J J Champoux; B L McConaughy
Journal: Biochemistry Date: 1975-01-28 Impact factor: 3.162

4. Nucleoprotein complexes from simian virus 40-infected monkey cells: association of viral DNA with histones and the major viral structural protein.

Authors: C C Howe; K B Tan
Journal: Virology Date: 1977-05-01 Impact factor: 3.616

1. Topoisomerase I is preferentially associated with normal SV40 replicative intermediates, but is associated with both replicating and nonreplicating SV40 DNAs which are deficient in histones.

Authors: J J Champoux
Journal: Nucleic Acids Res Date: 1992-07-11 Impact factor: 16.971

Interaction of the DNA untwisting enzyme with the SV40 nucleoprotein complex.

1. Folding of the DNA double helix in chromatin-like structures from simian virus 40.

2. Characterization of purified DNA-relaxing enzyme from human tissue culture cells.

3. Priming of superhelical SV40 DNA by Escherichia coli RNA polymerase for in vitro DNA synthesis.

4. Nucleoprotein complexes from simian virus 40-infected monkey cells: association of viral DNA with histones and the major viral structural protein.

5. Purification and characterization of the DNA untwisting enzyme from rat liver.

6. Stability of nucleosomes in native and reconstituted chromatins.

7. DNA gyrase: an enzyme that introduces superhelical turns into DNA.

8. The number of superhelical turns in native virion SV40 DNA and minicol DNA determined by the band counting method.

9. Salt and divalent cations affect the flexible nature of the natural beaded chromatin structure.

10. In vitro replication of simian virus 40 DNA in a nucleoprotein complex.

1. Topoisomerase I is preferentially associated with normal SV40 replicative intermediates, but is associated with both replicating and nonreplicating SV40 DNAs which are deficient in histones.

2. Polyoma virus minichromosomes: associated enzyme activities.

3. Circular dichroism and DNA secondary structure.

4. Simian virus 40 minichromosomes contain torsionally strained DNA molecules.

5. Nicking-closing enzyme is associated with SV40 DNA in vivo as a sodium dodecyl sulfate-resistant complex.

6. Prereplicative events involving simian virus 40 DNA in permissive cells.

7. Positively supercoiled plasmid DNA is produced by treatment of Escherichia coli with DNA gyrase inhibitors.