Literature DB >> 188018

Stability of nucleosomes in native and reconstituted chromatins.

J E Germond, M Bellard, P Oudet, P Chambon.   

Abstract

The stability of nucleosomes of SV40 minichromosomes extracted from infected cells or reconstituted by association of SV40 DNA and the four histones H2A, H2B, H3 and H4 was studied as a function of the ionic strength. As a measure of the stability of the nucleosome, we followed the disappearance of the nucleosomes from the original chromatin and their appearance on a "competing" DNA. We show here that the DNA and the histone components of the nucleosomes do not apprecially dissociate below 800 mM NaCl. At 800 mM and above, the histone moiety of the nucleosomes can dissociate from the DNA and efficiently participate to the formation of nucleosomes on a "competing" DNA.

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Year:  1976        PMID： 188018      PMCID： PMC343161          DOI： 10.1093/nar/3.11.3173

Source DB:  PubMed          Journal:  Nucleic Acids Res        ISSN： 0305-1048            Impact factor:   16.971


  7 in total

1.  Electron microscopic and biochemical evidence that chromatin structure is a repeating unit.

Authors:  P Oudet; M Gross-Bellard; P Chambon
Journal:  Cell       Date:  1975-04       Impact factor: 41.582

2.  Folding of the DNA double helix in chromatin-like structures from simian virus 40.

Authors:  J E Germond; B Hirt; P Oudet; M Gross-Bellark; P Chambon
Journal:  Proc Natl Acad Sci U S A       Date:  1975-05       Impact factor: 11.205

3.  Stepwise relaxation of supercoiled SV40 DNA.

Authors:  W Keller; I Wendel
Journal:  Cold Spring Harb Symp Quant Biol       Date:  1975

4.  Deficiency in histone acetylation in nontransforming host range mutants of polyoma virus.

Authors:  B S Schaffhausen; T L Benjamin
Journal:  Proc Natl Acad Sci U S A       Date:  1976-04       Impact factor: 11.205

5.  An octamer of histones in chromatin and free in solution.

Authors:  J O Thomas; R D Kornberg
Journal:  Proc Natl Acad Sci U S A       Date:  1975-07       Impact factor: 11.205

6.  Structure of chromatin.

Authors:  R J Clark; G Felsenfeld
Journal:  Nat New Biol       Date:  1971-01-27

7.  High resolution acrylamide gel electrophoresis of histones.

Authors:  S Panyim; R Chalkley
Journal:  Arch Biochem Biophys       Date:  1969-03       Impact factor: 4.013
  7 in total
  45 in total

1.  Extracts of Drosophila embryos mediate chromatin assembly in vitro.

Authors:  T Nelson; T S Hsieh; D Brutlag
Journal:  Proc Natl Acad Sci U S A       Date:  1979-11       Impact factor: 11.205

2.  Sin mutations alter inherent nucleosome mobility.

Authors:  Andrew Flaus; Chantal Rencurel; Helder Ferreira; Nicola Wiechens; Tom Owen-Hughes
Journal:  EMBO J       Date:  2004-01-15       Impact factor: 11.598

3.  Transcription of nucleosomal DNA in SV40 minichromosomes by eukaryotic and prokaryotic RNA polymerases.

Authors:  G Meneguzzi; N Chenciner; G Milanesi
Journal:  Nucleic Acids Res       Date:  1979-06-25       Impact factor: 16.971

4.  Nicking-closing enzyme assembles nucleosome-like structures in vitro.

Authors:  J E Germond; J Rouvière-Yaniv; M Yaniv; D Brutlag
Journal:  Proc Natl Acad Sci U S A       Date:  1979-08       Impact factor: 11.205

5.  The same amount of DNA is organized in in vitro-assembled nucleosomes irrespective of the origin of the histones.

Authors:  C Spadafora; P Oudet; P Chambon
Journal:  Nucleic Acids Res       Date:  1978-10       Impact factor: 16.971

6.  Nucleoprotein hybridization: a method for isolating active and inactive genes as chromatin.

Authors:  C Vincenz; J Fronk; G A Tank; J P Langmore
Journal:  Nucleic Acids Res       Date:  1991-03-25       Impact factor: 16.971

7.  Nuclease Bal-31 mapping of proteins bound to a tRNA(tyr) gene in SV40 minichromosomes.

Authors:  S R Scanlon; W R Folk
Journal:  Nucleic Acids Res       Date:  1991-12       Impact factor: 16.971

8.  Effect of histone acetylation on structure and in vitro transcription of chromatin.

Authors:  D J Mathis; P Oudet; B Wasylyk; P Chambon
Journal:  Nucleic Acids Res       Date:  1978-10       Impact factor: 16.971

9.  Transcription factor access is mediated by accurately positioned nucleosomes on the mouse mammary tumor virus promoter.

Authors:  T K Archer; M G Cordingley; R G Wolford; G L Hager
Journal:  Mol Cell Biol       Date:  1991-02       Impact factor: 4.272

10.  Mapping nucleosome position at single base-pair resolution by using site-directed hydroxyl radicals.

Authors:  A Flaus; K Luger; S Tan; T J Richmond
Journal:  Proc Natl Acad Sci U S A       Date:  1996-02-20       Impact factor: 11.205
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