Warning: Undefined array key "mm" in /www/wwwroot/www.ai-bt.com/si.php on line 10 Deprecated: trim(): Passing null to parameter #1 ($string) of type string is deprecated in /www/wwwroot/www.ai-bt.com/si.php on line 10 Purification and characterization of the DNA untwisting enzyme from rat liver.

Literature DB >> 184821

Purification and characterization of the DNA untwisting enzyme from rat liver.

Abstract

The DNA untwisting enzyme has been purified approximately 300-fold from rat liver nuclei. The protein is greater than 90% pure as judged by sodium dodecyl sulfate-acrylamide gel electrophoresis. The native enzyme has a molecular weight between 64 000 and 68 000 and is composed of a single polypeptide chain. Evidence is presented that the protein can act catalytically. A trace amount of endonuclease activity associated with the most pure fraction could be a contaminant or it could be due to the action of the DNA untwisting enzyme itself.

Entities: Chemical Species

Mesh：

Substances：

Year: 1976 PMID： 184821 DOI： 10.1021/bi00666a014

Source DB: PubMed Journal: Biochemistry ISSN： 0006-2960 Impact factor: 3.162

Keyword Cloud
Cited

33 in total

1. Luteolin, an emerging anti-cancer flavonoid, poisons eukaryotic DNA topoisomerase I.

Authors: Arnab Roy Chowdhury; Shalini Sharma; Suparna Mandal; Anindya Goswami; Sibabrata Mukhopadhyay; Hemanta K Majumder
Journal: Biochem J Date: 2002-09-01 Impact factor: 3.857

2. Topoisomerase I is preferentially associated with normal SV40 replicative intermediates, but is associated with both replicating and nonreplicating SV40 DNAs which are deficient in histones.

Authors: J J Champoux
Journal: Nucleic Acids Res Date: 1992-07-11 Impact factor: 16.971

6. Renaturation of complementary single-stranded DNA circles: complete rewinding facilitated by the DNA untwisting enzyme.

Authors: J J Champoux
Journal: Proc Natl Acad Sci U S A Date: 1977-12 Impact factor: 11.205

10. An RNA polymerase II transcription factor has an associated DNA-dependent ATPase (dATPase) activity strongly stimulated by the TATA region of promoters.

Authors: R C Conaway; J W Conaway
Journal: Proc Natl Acad Sci U S A Date: 1989-10 Impact factor: 11.205

Purification and characterization of the DNA untwisting enzyme from rat liver.

1. Luteolin, an emerging anti-cancer flavonoid, poisons eukaryotic DNA topoisomerase I.

2. Topoisomerase I is preferentially associated with normal SV40 replicative intermediates, but is associated with both replicating and nonreplicating SV40 DNAs which are deficient in histones.

3. Nicking-closing enzyme assembles nucleosome-like structures in vitro.

4. Strand breakage by the DNA untwisting enzyme results in covalent attachment of the enzyme to DNA.

5. An insight into the active site of a type I DNA topoisomerase from the kinetoplastid protozoan Leishmania donovani.

6. Renaturation of complementary single-stranded DNA circles: complete rewinding facilitated by the DNA untwisting enzyme.

7. DNA-relaxing enzyme from Micrococcus luteus.

8. Interaction of the DNA untwisting enzyme with the SV40 nucleoprotein complex.

9. Escherichia coli DNA topoisomerase I catalyzed linking of single-stranded rings of complementary base sequences.

10. An RNA polymerase II transcription factor has an associated DNA-dependent ATPase (dATPase) activity strongly stimulated by the TATA region of promoters.