Literature DB >> 170605

Characterization of purified DNA-relaxing enzyme from human tissue culture cells.

W Keller.   

Abstract

Superhelical simian virus 40 DNA migrates more rapidly during electrophoresis in agarose gels than covalently closed DNA free of superhelical turns (relaxed DNA). The difference in electrophoretic mobility between superhelical and relaxed DNA was used to monitor the activity of a protein from human tissue culture cells which converts superhelical DNA into relaxed DNA. Purified DNA-relaxing protein removes both negative and positive superhelical turns and acts in a catalytic manner. The relaxation of DNA proceeds in a stepwise fashion and DNA intermediates with decreasing numbers of superhelical turns are seen during the course of the reaction.

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Year:  1975        PMID： 170605      PMCID： PMC432806          DOI： 10.1073/pnas.72.7.2550

Source DB:  PubMed          Journal:  Proc Natl Acad Sci U S A        ISSN： 0027-8424            Impact factor:   11.205


  15 in total

1.  Stepwise relaxation of supercoiled SV40 DNA.

Authors:  W Keller; I Wendel
Journal:  Cold Spring Harb Symp Quant Biol       Date:  1975

2.  Detection of two restriction endonuclease activities in Haemophilus parainfluenzae using analytical agarose--ethidium bromide electrophoresis.

Authors:  P A Sharp; B Sugden; J Sambrook
Journal:  Biochemistry       Date:  1973-07-31       Impact factor: 3.162

3.  A buoyant method for the determination of the superhelix density of closed circular DNA.

Authors:  H B Gray; W B Upholt; J Vinograd
Journal:  J Mol Biol       Date:  1971-11-28       Impact factor: 5.469

4.  An activity from mammalian cells that untwists superhelical DNA--a possible swivel for DNA replication (polyoma-ethidium bromide-mouse-embryo cells-dye binding assay).

Authors:  J J Champoux; R Dulbecco
Journal:  Proc Natl Acad Sci U S A       Date:  1972-01       Impact factor: 11.205

5.  Encapsidation of free host DNA by simian virus 40: a simian virus 40 pseudovirus.

Authors:  D M Trilling; D Axelrod
Journal:  Science       Date:  1970-04-10       Impact factor: 47.728

6.  Amount of viral DNA in the genome of cells transformed by adenovirus type 2.

Authors:  U Pettersson; J Sambrook
Journal:  J Mol Biol       Date:  1973-01       Impact factor: 5.469

7.  Enzymatic synthesis of deoxyribonucleic acid. XXV. Purification and properties of deoxyribonucleic acid polymerase induced by infection with phage T4.

Authors:  M Goulian; Z J Lucas; A Kornberg
Journal:  J Biol Chem       Date:  1968-02-10       Impact factor: 5.157

8.  Mammalian deoxyribonucleic acid-dependent ribonucleic acid polymerases. I. Purification and properties of an -amanitin-sensitive ribonucleic acid polymerase and stimulatory factors from HeLa and KB cells.

Authors:  B Sugden; W Keller
Journal:  J Biol Chem       Date:  1973-06-10       Impact factor: 5.157

9.  Variation of the average rotation angle of the DNA helix and the superhelical turns of covalently closed cyclic lambda DNA.

Authors:  J C Wang
Journal:  J Mol Biol       Date:  1969-07-14       Impact factor: 5.469

10.  A fluorescent complex between ethidium bromide and nucleic acids. Physical-chemical characterization.

Authors:  J B LePecq; C Paoletti
Journal:  J Mol Biol       Date:  1967-07-14       Impact factor: 5.469
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  49 in total

1.  Restriction endonucleases: general survey procedure and survey of gliding bacteria.

Authors:  H Mayer; H Reichenbach
Journal:  J Bacteriol       Date:  1978-11       Impact factor: 3.490

2.  Groove-binding unsymmetrical cyanine dyes for staining of DNA: syntheses and characterization of the DNA-binding.

Authors:  H Jonas Karlsson; Maja Eriksson; Erik Perzon; Björn Akerman; Per Lincoln; Gunnar Westman
Journal:  Nucleic Acids Res       Date:  2003-11-01       Impact factor: 16.971

3.  Purification of a DNA nicking-closing enzyme from mouse L cells.

Authors:  D Tang
Journal:  Nucleic Acids Res       Date:  1978-08       Impact factor: 16.971

4.  Action of nicking-closing enzyme on supercoiled and nonsupercoiled closed circular DNA: formation of a Boltzmann distribution of topological isomers.

Authors:  D E Pulleyblank; M Shure; D Tang; J Vinograd; H P Vosberg
Journal:  Proc Natl Acad Sci U S A       Date:  1975-11       Impact factor: 11.205

5.  Evidence for an intermediate with a single-strand break in the reaction catalyzed by the DNA untwisting enzyme.

Authors:  J J Champoux
Journal:  Proc Natl Acad Sci U S A       Date:  1976-10       Impact factor: 11.205

6.  Influence of highly curved DNA segments on in vivo topology of plasmids.

Authors:  T Ohyama; M Miyano; S Sakuma
Journal:  Mol Biol Rep       Date:  1999-12       Impact factor: 2.316

7.  DNA gyrase: an enzyme that introduces superhelical turns into DNA.

Authors:  M Gellert; K Mizuuchi; M H O'Dea; H A Nash
Journal:  Proc Natl Acad Sci U S A       Date:  1976-11       Impact factor: 11.205

8.  Studies on the interaction of H1 histone with superhelical DNA: characterization of the recognition and binding regions of H1 histones.

Authors:  D S Singer; M F Singer
Journal:  Nucleic Acids Res       Date:  1976-10       Impact factor: 16.971

9.  DNA-relaxing activity and endonuclease activity in Xenopus laevis oocytes.

Authors:  E Mattoccia; D G Attardi; G P Tocchini-Valentini
Journal:  Proc Natl Acad Sci U S A       Date:  1976-12       Impact factor: 11.205

10.  Assembly of MMTV promoter minichromosomes with positioned nucleosomes precludes NF1 access but not restriction enzyme cleavage.

Authors:  P Venditti; L Di Croce; M Kauer; T Blank; P B Becker; M Beato
Journal:  Nucleic Acids Res       Date:  1998-08-15       Impact factor: 16.971
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