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Literature DB >> 20449256

DNA-polyfluorophore excimers as sensitive reporters for esterases and lipases.

Abstract

DNA-scaffolded oligodeoxyriboside fluorophores (ODFs) were used as the reporters in turn-on sensing of enzymatic bond-cleaving activity. A tetramer ODF of pyrene deoxynucleosides displayed high quenching efficiency when conjugated via ester linkages with a dabcyl quencher, and yielded large signal increases with several enzymes in vitro and in intact human cells.

Entities: CellLine Chemical Gene Species

Mesh：

Substances：

Year: 2010 PMID： 20449256 PMCID： PMC5501962 DOI： 10.1039/b926338a

Source DB: PubMed Journal: Chem Commun (Camb) ISSN： 1359-7345 Impact factor: 6.222

31 in total

1. Low background FRET-substrates for lipases and esterases suitable for high-throughput screening under basic (pH 11) conditions.

Authors: Yongzheng Yang; Peter Babiak; Jean-Louis Reymond
Journal: Org Biomol Chem Date: 2006-04-03 Impact factor: 3.876

2. Novel fluorogenic substrates for imaging beta-lactamase gene expression.

Authors: Wenzhong Gao; Bengang Xing; Roger Y Tsien; Jianghong Rao
Journal: J Am Chem Soc Date: 2003-09-17 Impact factor: 15.419

3. Polyfluorophores on a DNA backbone: a multicolor set of labels excited at one wavelength.

Authors: Yin Nah Teo; James N Wilson; Eric T Kool
Journal: J Am Chem Soc Date: 2009-03-25 Impact factor: 15.419

4. A red-fluorescent substrate microarray for lipase fingerprinting.

Authors: Johann Grognux; Jean-Louis Reymond
Journal: Mol Biosyst Date: 2006-08-24

5. Fluorogenic substrates for lipases, esterases, and acylases using a TIM-mechanism for signal release.

Authors: Renaud Sicart; Marie-Pierre Collin; Jean-Louis Reymond
Journal: Biotechnol J Date: 2007-02 Impact factor: 4.677

Review 6. Acetylcholinesterase inhibitors as disease-modifying therapies for Alzheimer's disease.

Authors: D Muñoz-Torrero
Journal: Curr Med Chem Date: 2008 Impact factor: 4.530

Review 7. Recent advances in enzyme assays.

Authors: Jean-Philippe Goddard; Jean-Louis Reymond
Journal: Trends Biotechnol Date: 2004-07 Impact factor: 19.536

Review 8. Overview of the chemical families of fatty acid amide hydrolase and monoacylglycerol lipase inhibitors.

Authors: Séverine Vandevoorde
Journal: Curr Top Med Chem Date: 2008 Impact factor: 3.295

9. Oligodeoxyfluorosides: Strong Sequence Dependence of Fluorescence Emission.

Authors: James N Wilson; Jianmin Gao; Eric T Kool
Journal: Tetrahedron Date: 2007-04-23 Impact factor: 2.457

10. Polyfluorophore excimers and exciplexes as FRET donors in DNA.

Authors: Yin Nah Teo; Eric T Kool
Journal: Bioconjug Chem Date: 2009-12 Impact factor: 4.774

11 in total

Review 1. DNA-multichromophore systems.

Authors: Yin Nah Teo; Eric T Kool
Journal: Chem Rev Date: 2012-03-16 Impact factor: 60.622

DNA-polyfluorophore excimers as sensitive reporters for esterases and lipases.

1. Low background FRET-substrates for lipases and esterases suitable for high-throughput screening under basic (pH 11) conditions.

2. Novel fluorogenic substrates for imaging beta-lactamase gene expression.

3. Polyfluorophores on a DNA backbone: a multicolor set of labels excited at one wavelength.

4. A red-fluorescent substrate microarray for lipase fingerprinting.

5. Fluorogenic substrates for lipases, esterases, and acylases using a TIM-mechanism for signal release.

Review 6. Acetylcholinesterase inhibitors as disease-modifying therapies for Alzheimer's disease.

Review 7. Recent advances in enzyme assays.

Review 8. Overview of the chemical families of fatty acid amide hydrolase and monoacylglycerol lipase inhibitors.

9. Oligodeoxyfluorosides: Strong Sequence Dependence of Fluorescence Emission.

10. Polyfluorophore excimers and exciplexes as FRET donors in DNA.

Review 1. DNA-multichromophore systems.

Review 2. Chemistry of nucleic acids: impacts in multiple fields.

3. Direct fluorescence monitoring of DNA base excision repair.

4. Multi-path quenchers: efficient quenching of common fluorophores.

5. Protease probes built from DNA: multispectral fluorescent DNA-peptide conjugates as caspase chemosensors.

6. Differentiating between fluorescence-quenching metal ions with polyfluorophore sensors built on a DNA backbone.

Review 7. Fluorescent DNA-based enzyme sensors.

8. Multispectral labeling of antibodies with polyfluorophores on a DNA backbone and application in cellular imaging.

9. Genetically encoded multispectral labeling of proteins with polyfluorophores on a DNA backbone.

10. Selective fluorogenic chemosensors for distinct classes of nucleases.