Literature DB >> 16220906

Novel fluorogenic substrates for imaging beta-lactamase gene expression.

Wenzhong Gao1, Bengang Xing, Roger Y Tsien, Jianghong Rao.   

Abstract

A new class of small nonfluorescent fluorogenic substrates becomes brightly fluorescent after beta-lactamase hydrolysis with up to 153-fold enhancement in the fluorescence intensity. Less than 500 fM of beta-lactamase in cell lysates can be readily detected, and beta-lactamase expression in living cells can be imaged with a red fluorescence derivative. These new fluorogenic substrates should find uses in clinical diagnostics and facilitate the applications of beta-lactamase as a biosensor.

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Year:  2003        PMID： 16220906     DOI： 10.1021/ja036126o

Source DB:  PubMed          Journal:  J Am Chem Soc        ISSN： 0002-7863            Impact factor:   15.419


  34 in total

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