Literature DB >> 20445273

Trial by fire: are the crystals macromolecules?

Kannan Raghunathan1, Paul T Harris, Dennis N Arvidson.   

Abstract

Protein crystallization screens frequently yield salt crystals as well as protein crystals. A simple method for determining whether a crystal is composed of salt or macromolecules is suggested. A drop containing one or more crystals is transferred to a glass cover slip and the cover slip is then passed through the flame of a Bunsen burner. Macromolecule crystals are destroyed by this treatment, while salt crystals generally remain. The test can be performed after other commonly used tests such as crushing and staining.

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Year:  2010        PMID： 20445273      PMCID： PMC2864706          DOI： 10.1107/S1744309110012078

Source DB:  PubMed          Journal:  Acta Crystallogr Sect F Struct Biol Cryst Commun        ISSN： 1744-3091


  15 in total

1.  The prospects of protein nanocrystallography.

Authors:  E Rene Bodenstaff; Flip J Hoedemaeker; Maxim E Kuil; Hans P M de Vrind; Jan Pieter Abrahams
Journal:  Acta Crystallogr D Biol Crystallogr       Date:  2002-10-21

2.  INTERMOLECULAR CROSS LINKING OF A PROTEIN IN THE CRYSTALLINE STATE: CARBOXYPEPTIDASE-A.

Authors:  F A QUIOCHO; F M RICHARDS
Journal:  Proc Natl Acad Sci U S A       Date:  1964-09       Impact factor: 11.205

3.  Assessing crystallization droplets using birefringence.

Authors:  A Echalier; R L Glazer; V Fülöp; M A Geday
Journal:  Acta Crystallogr D Biol Crystallogr       Date:  2004-03-23

4.  Efficient UV detection of protein crystals enabled by fluorescence excitation at wavelengths longer than 300 nm.

Authors:  Karsten Dierks; Arne Meyer; Dominik Oberthür; Gert Rapp; Howard Einspahr; Christian Betzel
Journal:  Acta Crystallogr Sect F Struct Biol Cryst Commun       Date:  2010-03-27

5.  An ultraviolet fluorescence-based method for identifying and distinguishing protein crystals.

Authors:  Russell A Judge; Kerry Swift; Carlos González
Journal:  Acta Crystallogr D Biol Crystallogr       Date:  2004-12-17

6.  Structure of an RNA polymerase II-RNA inhibitor complex elucidates transcription regulation by noncoding RNAs.

Authors:  Hubert Kettenberger; Alexander Eisenführ; Florian Brueckner; Mirko Theis; Michael Famulok; Patrick Cramer
Journal:  Nat Struct Mol Biol       Date:  2005-12-11       Impact factor: 15.369

7.  Screening and optimization methods for nonautomated crystallization laboratories.

Authors:  Terese Bergfors
Journal:  Methods Mol Biol       Date:  2007

8.  A method for the general identification of protein crystals in crystallization experiments using a noncovalent fluorescent dye.

Authors:  Matthew R Groves; Ingrid B Müller; Xandra Kreplin; Jochen Müller-Dieckmann
Journal:  Acta Crystallogr D Biol Crystallogr       Date:  2007-03-16

9.  Trace fluorescent labeling for high-throughput crystallography.

Authors:  Elizabeth Forsythe; Aniruddha Achari; Marc L Pusey
Journal:  Acta Crystallogr D Biol Crystallogr       Date:  2006-02-22

10.  Crystal structure of the bacterial cell-division protein FtsZ.

Authors:  J Löwe; L A Amos
Journal:  Nature       Date:  1998-01-08       Impact factor: 49.962
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  2 in total

1.  Expression, purification, crystallization and preliminary X-ray studies of Lactobacillus jensenii enolase.

Authors:  Paul T Harris; Kannan Raghunathan; Rachel R Spurbeck; Cindy G Arvidson; Dennis N Arvidson
Journal:  Acta Crystallogr Sect F Struct Biol Cryst Commun       Date:  2010-07-29

2.  Improved protein-crystal identification by using 2,2,2-trichloroethanol as a fluorescence enhancer.

Authors:  Christian Pichlo; Christine Toelzer; Konrad Chojnacki; Sinan Öcal; Matthias Uthoff; Sabine Ruegenberg; Thomas Hermanns; Magdalena Schacherl; Martin S Denzel; Kay Hofmann; Karsten Niefind; Ulrich Baumann
Journal:  Acta Crystallogr F Struct Biol Commun       Date:  2018-04-24       Impact factor: 1.056
  2 in total

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