Literature DB >> 2038049

Time-resolved cryo-electron microscopic study of the dissociation of actomyosin induced by photolysis of photolabile nucleotides.

J F Ménétret1, W Hofmann, R R Schröder, G Rapp, R S Goody.   

Abstract

The rapid release of a substrate or other ligand from photolabile precursors in a thin layer suspension of biological specimens followed by rapid freezing provides a method of trapping and visualizing short-lived states in a dynamic system. We demonstrate here the first successful application of this method to study the interaction of actin filaments with myosin subfragment 1 (S1) after release of nucleotides. The results obtained suggest that structural changes in actin filaments occur as a result of interaction with S1.

Mesh：

Substances：

Year:  1991        PMID： 2038049     DOI： 10.1016/0022-2836(91)90554-j

Source DB:  PubMed          Journal:  J Mol Biol        ISSN： 0022-2836            Impact factor:   5.469


  14 in total

Review 1.  Two promising future developments of cryo-EM: capturing short-lived states and mapping a continuum of states of a macromolecule.

Authors:  Bo Chen; Joachim Frank
Journal:  Microscopy (Oxf)       Date:  2015-10-31       Impact factor: 1.571

2.  Muscle contraction and in vitro movement: role of actin?

Authors:  J E Morel; Z Merah
Journal:  J Muscle Res Cell Motil       Date:  1992-02       Impact factor: 2.698

3.  Unfixed cryosections of striated muscle to study dynamic molecular events.

Authors:  J F Ménétret; R Craig
Journal:  Biophys J       Date:  1994-10       Impact factor: 4.033

4.  Gas-Assisted Annular Microsprayer for Sample Preparation for Time-Resolved Cryo-Electron Microscopy.

Authors:  Zonghuan Lu; David Barnard; Tanvir R Shaikh; Xing Meng; Carmen A Mannella; Aymen Yassin; Rajendra Agrawal; Terence Wagenknecht; Toh-Ming Lu
Journal:  J Micromech Microeng       Date:  2014-11-01       Impact factor: 1.881

Review 5.  The alpha-helix, an overlooked molecular motor.

Authors:  R Jarosch
Journal:  Protoplasma       Date:  2005-12-30       Impact factor: 3.356

6.  Structural implications of the chemical modification of Cys(10) on actin.

Authors:  L Eli-Berchoer; E Reisler; A Muhlrad
Journal:  Biophys J       Date:  2000-03       Impact factor: 4.033

7.  Direct visualization by electron microscopy of the weakly bound intermediates in the actomyosin adenosine triphosphatase cycle.

Authors:  T D Pollard; D Bhandari; P Maupin; D Wachsstock; A G Weeds; H G Zot
Journal:  Biophys J       Date:  1993-02       Impact factor: 4.033

8.  Monolithic microfluidic mixing-spraying devices for time-resolved cryo-electron microscopy.

Authors:  Zonghuan Lu; Tanvir R Shaikh; David Barnard; Xing Meng; Hisham Mohamed; Aymen Yassin; Carmen A Mannella; Rajendra K Agrawal; Toh-Ming Lu; Terence Wagenknecht
Journal:  J Struct Biol       Date:  2009-08-14       Impact factor: 2.867

9.  Implementation of a flash-photolysis system for time-resolved cryo-electron microscopy.

Authors:  Tanvir R Shaikh; David Barnard; Xing Meng; Terence Wagenknecht
Journal:  J Struct Biol       Date:  2008-12-10       Impact factor: 2.867

Review 10.  Understanding the invisible hands of sample preparation for cryo-EM.

Authors:  Giulia Weissenberger; Rene J M Henderikx; Peter J Peters
Journal:  Nat Methods       Date:  2021-05-07       Impact factor: 47.990
View more

北京卡尤迪生物科技股份有限公司 © 2022-2023.