Literature DB >> 2023922

Sites of interaction of the CCA end of peptidyl-tRNA with 23S rRNA.

D Moazed1, H F Noller.   

Abstract

Oligonucleotide fragments derived from the 3' CCA terminus of acylated tRNA, such as CACCA-(AcPhe), UACCA-(AcLeu), and CAACCA-(fMet), bind specifically to ribosomes in the presence of sparsomycin and methanol [Monro, R. E., Celma, M. L. & Vazquez, D. (1969) Nature (London) 222, 356-358]. All three oligonucleotides protect a characteristic set of bases in 23S rRNA from chemical probes: G2252, G2253, A2439, A2451, U2506, and U2585. A2602 shows enhanced reactivity. These account for most of the same bases that are protected when peptidyl-tRNA analogues such as AcPhe-tRNA are bound to the ribosomal P site, and correspond precisely to those bases whose protection is abolished by removal of the 3'-CA end of tRNA. We conclude that most of the observed interactions between tRNA and 23S rRNA in the 50S ribosomal P site involve the conserved CCA terminus of tRNA. Sparsomycin may inhibit protein synthesis by stabilizing interaction between the peptidyl-CCA and the 23S P site, preventing formation of the intermediate A/P hybrid state.

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Year:  1991        PMID： 2023922      PMCID： PMC51525          DOI： 10.1073/pnas.88.9.3725

Source DB:  PubMed          Journal:  Proc Natl Acad Sci U S A        ISSN： 0027-8424            Impact factor:   11.205


  17 in total

1.  Transfer RNA shields specific nucleotides in 16S ribosomal RNA from attack by chemical probes.

Authors:  D Moazed; H F Noller
Journal:  Cell       Date:  1986-12-26       Impact factor: 41.582

2.  Binding of tRNA to the ribosomal A and P sites protects two distinct sets of nucleotides in 16 S rRNA.

Authors:  D Moazed; H F Noller
Journal:  J Mol Biol       Date:  1990-01-05       Impact factor: 5.469

3.  Interaction of antibiotics with functional sites in 16S ribosomal RNA.

Authors:  D Moazed; H F Noller
Journal:  Nature       Date:  1987 Jun 4-10       Impact factor: 49.962

4.  The behaviour of acetylphenylalanyl soluble ribonucleic acid in polyphenylalanine synthesis.

Authors:  A L Haenni; F Chapeville
Journal:  Biochim Biophys Acta       Date:  1966-01-18

5.  Action of sparsomycin on ribosome-catalysed peptidyl transfer.

Authors:  R E Monro; M L Celma; D Vazquez
Journal:  Nature       Date:  1969-04-26       Impact factor: 49.962

6.  An apparent conformational change in tRNA(Phe) that is associated with the peptidyl transferase reaction.

Authors:  O W Odom; B Hardesty
Journal:  Biochimie       Date:  1987-09       Impact factor: 4.079

7.  Identification of a site on 23S ribosomal RNA located at the peptidyl transferase center.

Authors:  A Barta; G Steiner; J Brosius; H F Noller; E Kuechler
Journal:  Proc Natl Acad Sci U S A       Date:  1984-06       Impact factor: 11.205

8.  Covalent crosslinking of tRNA1Val to 16S RNA at the ribosomal P site: identification of crosslinked residues.

Authors:  J B Prince; B H Taylor; D L Thurlow; J Ofengand; R A Zimmermann
Journal:  Proc Natl Acad Sci U S A       Date:  1982-09       Impact factor: 11.205

9.  Altered ribosomal RNA genes in mitochondria from mammalian cells with chloramphenicol resistance.

Authors:  S E Kearsey; I W Craig
Journal:  Nature       Date:  1981-04-16       Impact factor: 49.962

10.  Chloramphenicol, erythromycin, carbomycin and vernamycin B protect overlapping sites in the peptidyl transferase region of 23S ribosomal RNA.

Authors:  D Moazed; H F Noller
Journal:  Biochimie       Date:  1987-08       Impact factor: 4.079
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  61 in total

1.  Peptidyl transferase antibiotics perturb the relative positioning of the 3'-terminal adenosine of P/P'-site-bound tRNA and 23S rRNA in the ribosome.

Authors:  S V Kirillov; B T Porse; R A Garrett
Journal:  RNA       Date:  1999-08       Impact factor: 4.942

2.  Interactions between 23S rRNA and tRNA in the ribosomal E site.

Authors:  M Bocchetta; L Xiong; S Shah; A S Mankin
Journal:  RNA       Date:  2001-01       Impact factor: 4.942

3.  Novel mutants of 23S RNA: characterization of functional properties.

Authors:  U Saarma; J Remme
Journal:  Nucleic Acids Res       Date:  1992-06-25       Impact factor: 16.971

Review 4.  Ribosome evolution: emergence of peptide synthesis machinery.

Authors:  Koji Tamura
Journal:  J Biosci       Date:  2011-12       Impact factor: 1.826

5.  Evolution of protein synthesis from an RNA world.

Authors:  Harry F Noller
Journal:  Cold Spring Harb Perspect Biol       Date:  2012-04-01       Impact factor: 10.005

6.  How are tRNAs and mRNA arranged in the ribosome? An attempt to correlate the stereochemistry of the tRNA-mRNA interaction with constraints imposed by the ribosomal topography.

Authors:  V Lim; C Venclovas; A Spirin; R Brimacombe; P Mitchell; F Müller
Journal:  Nucleic Acids Res       Date:  1992-06-11       Impact factor: 16.971

7.  Substitution of the 3' terminal adenosine residue of transfer RNA in vivo.

Authors:  N B Reuven; M P Deutscher
Journal:  Proc Natl Acad Sci U S A       Date:  1993-05-15       Impact factor: 11.205

8.  3'-immature tRNA(Trp) is required for ribosome inactivation by gelonin,a plant RNA N-glycosidase.

Authors:  M Brigotti; D Carnicelli; P Alvergna; A Pallanca; R Lorenzetti; M Denaro; S Sperti; L Montanaro
Journal:  Biochem J       Date:  1995-08-15       Impact factor: 3.857

9.  Exploration of the conserved A+C wobble pair within the ribosomal peptidyl transferase center using affinity purified mutant ribosomes.

Authors:  Ashley Eversole Hesslein; Vladimir I Katunin; Malte Beringer; Anne B Kosek; Marina V Rodnina; Scott A Strobel
Journal:  Nucleic Acids Res       Date:  2004-07-15       Impact factor: 16.971

10.  Site-directed cross-linking studies on the E. coli tRNA-ribosome complex: determination of sites labelled with an aromatic azide attached to the variable loop or aminoacyl group of tRNA.

Authors:  P Mitchell; K Stade; M Osswald; R Brimacombe
Journal:  Nucleic Acids Res       Date:  1993-02-25       Impact factor: 16.971
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