Literature DB >> 11214181

Interactions between 23S rRNA and tRNA in the ribosomal E site.

M Bocchetta1, L Xiong, S Shah, A S Mankin.   

Abstract

Interactions between tRNA or its analogs and 23S rRNA in the large ribosomal subunit were analyzed by RNA footprinting and by modification-interference selection. In the E site, tRNA protected bases G2112, A2392, and C2394 of 23S rRNA. Truncated tRNA, lacking the anticodon stem-loop, protected A2392 and C2394, but not G2112, and tRNA derivatives with a shortened 3' end protected only G2112, but not A2392 or C2394. Modification interference revealed C2394 as the only accessible nucleotide in 23S rRNA whose modification interferes with binding of tRNA in the large ribosomal subunit E site. The results suggest a direct contact between A76 of tRNA A76 and C2394 of 23S rRNA. Protections at G2112 may reflect interaction of this 23S rRNA region with the tRNA central fold.

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Year:  2001        PMID： 11214181      PMCID： PMC1370069          DOI： 10.1017/s1355838201001650

Source DB:  PubMed          Journal:  RNA        ISSN： 1355-8382            Impact factor:   4.942


  35 in total

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Journal:  Biochimie       Date:  1995       Impact factor: 4.079

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Authors:  M Bocchetta; L Xiong; A S Mankin
Journal:  Proc Natl Acad Sci U S A       Date:  1998-03-31       Impact factor: 11.205

5.  The donor substrate site within the peptidyl transferase loop of 23 S rRNA and its putative interactions with the CCA-end of N-blocked aminoacyl-tRNA(Phe).

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Journal:  J Mol Biol       Date:  1996-12-06       Impact factor: 5.469

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Authors:  J Rinke-Appel; N Jünke; M Osswald; R Brimacombe
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Authors:  S Joseph; H F Noller
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  7 in total

1.  Participation of the tRNA A76 hydroxyl groups throughout translation.

Authors:  Joshua S Weinger; Scott A Strobel
Journal:  Biochemistry       Date:  2006-05-16       Impact factor: 3.162

2.  Messenger RNA conformations in the ribosomal E site revealed by X-ray crystallography.

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3.  Role of hybrid tRNA-binding states in ribosomal translocation.

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4.  Exploration of the conserved A+C wobble pair within the ribosomal peptidyl transferase center using affinity purified mutant ribosomes.

Authors:  Ashley Eversole Hesslein; Vladimir I Katunin; Malte Beringer; Anne B Kosek; Marina V Rodnina; Scott A Strobel
Journal:  Nucleic Acids Res       Date:  2004-07-15       Impact factor: 16.971

5.  Structures of deacylated tRNA mimics bound to the E site of the large ribosomal subunit.

Authors:  T Martin Schmeing; Peter B Moore; Thomas A Steitz
Journal:  RNA       Date:  2003-11       Impact factor: 4.942

6.  Function of the ribosomal E-site: a mutagenesis study.

Authors:  Petr V Sergiev; Dmitry V Lesnyak; Sergey V Kiparisov; Dmitry E Burakovsky; Andrei A Leonov; Alexey A Bogdanov; Richard Brimacombe; Olga A Dontsova
Journal:  Nucleic Acids Res       Date:  2005-10-20       Impact factor: 16.971

7.  The integrity of the G2421-C2395 base pair in the ribosomal E-site is crucial for protein synthesis.

Authors:  Miriam Koch; Nina Clementi; Nicola Rusca; Paul Vögele; Matthias Erlacher; Norbert Polacek
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  7 in total

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