Literature DB >> 2670677

Cloning of the nitrate reductase gene (niaD) of Aspergillus nidulans and its use for transformation of Fusarium oxysporum.

L Malardier1, M J Daboussi, J Julien, F Roussel, C Scazzocchio, Y Brygoo.   

Abstract

An heterologous transformation system for the phytopathogenic fungus Fusarium oxysporum has been developed based on the use of the Aspergillus nidulans nitrate reductase gene (niaD). F. oxysporum nia- mutants were easily selected by chlorate resistance. The A. nidulans niaD gene was isolated from a gene library by complementation of an A. nidulans niaD mutant. The cloned gene is capable of transforming F. oxysporum nia- mutants at a frequency of up to ten transformants per microgram of DNA. Southern analysis of the DNA of the F. oxysporum transformants showed that transformation resulted in integration of one or more copies of the vector DNA into the genome.

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Year:  1989        PMID： 2670677     DOI： 10.1016/0378-1119(89)90322-3

Source DB:  PubMed          Journal:  Gene        ISSN： 0378-1119            Impact factor:   3.688


  30 in total

1.  Fot1, a new family of fungal transposable elements.

Authors:  M J Daboussi; T Langin; Y Brygoo
Journal:  Mol Gen Genet       Date:  1992-03

2.  Novel polyketide synthase from Nectria haematococca.

Authors:  Stephane Graziani; Christelle Vasnier; Marie-Josee Daboussi
Journal:  Appl Environ Microbiol       Date:  2004-05       Impact factor: 4.792

3.  Nuclear export of the transcription factor NirA is a regulatory checkpoint for nitrate induction in Aspergillus nidulans.

Authors:  Andreas Bernreiter; Ana Ramon; Javier Fernández-Martínez; Harald Berger; Lidia Araújo-Bazan; Eduardo A Espeso; Robert Pachlinger; Andreas Gallmetzer; Ingund Anderl; Claudio Scazzocchio; Joseph Strauss
Journal:  Mol Cell Biol       Date:  2006-11-20       Impact factor: 4.272

4.  Improvement of the fatty acid composition of an oil-producing filamentous fungus, Mortierella alpina 1S-4, through RNA interference with delta12-desaturase gene expression.

Authors:  Seiki Takeno; Eiji Sakuradani; Akiko Tomi; Misa Inohara-Ochiai; Hiroshi Kawashima; Toshihiko Ashikari; Sakayu Shimizu
Journal:  Appl Environ Microbiol       Date:  2005-09       Impact factor: 4.792

5.  Molecular evidence that the rate-limiting step for the biosynthesis of arachidonic acid in Mortierella alpina is at the level of an elongase.

Authors:  Seiki Takeno; Eiji Sakuradani; Shoichi Murata; Misa Inohara-Ochiai; Hiroshi Kawashima; Toshihiko Ashikari; Sakayu Shimizu
Journal:  Lipids       Date:  2005-01       Impact factor: 1.880

6.  Cloning and expression of a chitinase gene from the hyperparasitic fungus Aphanocladium album.

Authors:  P L Blaiseau; C Kunz; R Grison; Y Bertheau; Y Brygoo
Journal:  Curr Genet       Date:  1992-01       Impact factor: 3.886

7.  Transposition of a fungal miniature inverted-repeat transposable element through the action of a Tc1-like transposase.

Authors:  Marie Dufresne; Aurélie Hua-Van; Hala Abd El Wahab; Sarrah Ben M'Barek; Christelle Vasnier; Laure Teysset; Gert H J Kema; Marie-Josée Daboussi
Journal:  Genetics       Date:  2006-12-18       Impact factor: 4.562

8.  Molecular cloning and functional characterization of the pathway-specific regulatory gene nirA, which controls nitrate assimilation in Aspergillus nidulans.

Authors:  G Burger; J Tilburn; C Scazzocchio
Journal:  Mol Cell Biol       Date:  1991-02       Impact factor: 4.272

9.  Genome-wide analysis of the Fusarium oxysporum mimp family of MITEs and mobilization of both native and de novo created mimps.

Authors:  Mara Bergemann; Olivier Lespinet; Sarrah Ben M'Barek; Marie-Josée Daboussi; Marie Dufresne
Journal:  J Mol Evol       Date:  2008-12       Impact factor: 2.395

10.  The GUS gene fusion system (Escherichia coli beta-D-glucuronidase gene), a useful tool in studies of root colonization by Fusarium oxysporum.

Authors:  Y Couteaudier; M J Daboussi; A Eparvier; T Langin; J Orcival
Journal:  Appl Environ Microbiol       Date:  1993-06       Impact factor: 4.792
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