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Literature DB >> 1327547

Transformation of Gibberella fujikuroi: effect of the Aspergillus nidulans AMA1 sequence on frequency and integration.

B Brückner¹, S E Unkles, K Weltring, J R Kinghorn.

Abstract

A stable and reproducible transformation selection system for Gibberella fujikuroi protoplasts based on the Aspergillus nidulans arg B gene, encoding ornithine transcarbamylase, has been developed. Inclusion into the vector of the A. nidulans DNA fragment (AMA1), which permits plasmid autonomous replication in A. nidulans, A. niger and A. oryzae, appeared to permit autonomous replication of G. fujikuroi although the transformation frequency was increased by only two-fold. Transformation was also achieved using the bacterial hygromycin B resistance gene under the control of G. pulicaris and A. nidulans promoters.

Entities: Chemical Species

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Year: 1992 PMID： 1327547 DOI： 10.1007/bf00317927

Source DB: PubMed Journal: Curr Genet ISSN： 0172-8083 Impact factor: 3.886

7 in total

1. Transformation of Aspergillus based on the hygromycin B resistance marker from Escherichia coli.

Authors: P J Punt; R P Oliver; M A Dingemanse; P H Pouwels; C A van den Hondel
Journal: Gene Date: 1987 Impact factor: 3.688

2. Transformation of Aspergillus nidulans with the hygromycin-resistance gene, hph.

Authors: D Cullen; S A Leong; L J Wilson; D J Henner
Journal: Gene Date: 1987 Impact factor: 3.688

3. An autonomously replicating plasmid transforms Aspergillus nidulans at high frequency.

Authors: D Gems; I L Johnstone; A J Clutterbuck
Journal: Gene Date: 1991-02-01 Impact factor: 3.688

4. Transformation of the filamentous fungus Gibberella fujikuroi using the Aspergillus niger niaD gene encoding nitrate reductase.

Authors: R Sànchez-Fernàndez; S E Unkles; E I Campbell; J A Macro; E Cerdà-Olmedo; J R Kinghorn
Journal: Mol Gen Genet Date: 1991-02

5. Transformation of Penicillium chrysogenum using dominant selection markers and expression of an Escherichia coli lacZ fusion gene.

Authors: M Kolar; P J Punt; C A van den Hondel; H Schwab
Journal: Gene Date: 1988 Impact factor: 3.688

6. Fate of DNA encoding hygromycin resistance after meiosis in transformed strains of Gibberella fujikuroi (Fusarium moniliforme).

Authors: J F Leslie; M B Dickman
Journal: Appl Environ Microbiol Date: 1991-05 Impact factor: 4.792

7. Cloning an Aspergillus nidulans developmental gene by transformation.

Authors: I L Johnstone; S G Hughes; A J Clutterbuck
Journal: EMBO J Date: 1985-05 Impact factor: 11.598

7 in total

11 in total

1. Transient and multivariate system for transformation of a fungal plant pathogen, Rosellinia necatrix, using autonomously replicating vectors.

Authors: Takeo Shimizu; Tsutae Ito; Satoko Kanematsu
Journal: Curr Genet Date: 2012-03-03 Impact factor: 3.886

Transformation of Gibberella fujikuroi: effect of the Aspergillus nidulans AMA1 sequence on frequency and integration.

1. Transformation of Aspergillus based on the hygromycin B resistance marker from Escherichia coli.

2. Transformation of Aspergillus nidulans with the hygromycin-resistance gene, hph.

3. An autonomously replicating plasmid transforms Aspergillus nidulans at high frequency.

4. Transformation of the filamentous fungus Gibberella fujikuroi using the Aspergillus niger niaD gene encoding nitrate reductase.

5. Transformation of Penicillium chrysogenum using dominant selection markers and expression of an Escherichia coli lacZ fusion gene.

6. Fate of DNA encoding hygromycin resistance after meiosis in transformed strains of Gibberella fujikuroi (Fusarium moniliforme).

7. Cloning an Aspergillus nidulans developmental gene by transformation.

1. Transient and multivariate system for transformation of a fungal plant pathogen, Rosellinia necatrix, using autonomously replicating vectors.

2. Autonomously replicating plasmids carrying the AMA1 region in Penicillium chrysogenum.

3. Integrative and replicative transformation of Penicillium canescens with a heterologous nitrate-reductase gene.

4. Efficient and versatile transformation systems in entomopathogenic fungus Lecanicillium species.

5. Transformation of the plant pathogenic fungus, Rhynchosporium secalis.

Review 6. The Thom Award address. Industrial mycology and the new genetics.

7. Heterologous transformation of Zalerion arboricola.

Review 8. Genetics and gibberellin production in Gibberella fujikuroi.

Review 9. The Gibberellin Producer Fusarium fujikuroi: Methods and Technologies in the Current Toolkit.

10. A novel fungal gene regulation system based on inducible VPR-dCas9 and nucleosome map-guided sgRNA positioning.