Literature DB >> 2673557

Transformation of seven species of filamentous fungi using the nitrate reductase gene of Aspergillus nidulans.

M J Daboussi1, A Djeballi, C Gerlinger, P L Blaiseau, I Bouvier, M Cassan, M H Lebrun, D Parisot, Y Brygoo.   

Abstract

A gene transfer system originally developed for Fusarium oxysporum has been applied to seven species of filamentous fungi of agricultural and industrial importance. This transformation system relies on the selection of mutants deficient in nitrate reductase by positive screening. Such mutants were recovered easily in all the fungi tested--without mutagenic treatments--through their resistance to chlorate. They were transformed by a plasmid vector (pAN301) carrying the Aspergillus nidulans wild-type gene (niaD). Transformation frequencies ranged from one to ten transformants/micrograms plasmid DNA. The general properties of the transformants were analyzed. Most of them are mitotically stable, and the integration of the vector into the host genome frequently occurred in a tandem fashion.

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Year:  1989        PMID： 2673557     DOI： 10.1007/bf00376803

Source DB:  PubMed          Journal:  Curr Genet        ISSN： 0172-8083            Impact factor:   3.886


  16 in total

1.  Induction and Characterization of Penicillium caseicolum Mutants Resistant to Ergosterol Biosynthesis Inhibitors.

Authors:  A de Falandre; I Bouvier-Fourcade; J M Seng; P Leroux
Journal:  Appl Environ Microbiol       Date:  1987-07       Impact factor: 4.792

2.  Positive screening and transformation of ura5 mutants in the fungus Podospora anserina: characterization of the transformants.

Authors:  V Razanamparany; J Bégueret
Journal:  Curr Genet       Date:  1986       Impact factor: 3.886

3.  Characterization of the Heterokaryotic and Vegetative Diploid Phases of MAGNAPORTHE GRISEA.

Authors:  M S Crawford; F G Chumley; C G Weaver; B Valent
Journal:  Genetics       Date:  1986-12       Impact factor: 4.562

4.  Nitrate reduction mutants of fusarium moniliforme (gibberella fujikuroi).

Authors:  C Klittich; J F Leslie
Journal:  Genetics       Date:  1988-03       Impact factor: 4.562

5.  Selectable genes for transformation of the fungal plant pathogen Glomerella cingulata f. sp. phaseoli (Colletotrichum lindemuthianum).

Authors:  R J Rodriguez; O C Yoder
Journal:  Gene       Date:  1987       Impact factor: 3.688

6.  Transformation of Aspergillus nidulans with a cloned, oligomycin-resistant ATP synthase subunit 9 gene.

Authors:  M Ward; B Wilkinson; G Turner
Journal:  Mol Gen Genet       Date:  1986-02

7.  Genetic transformation of the fungal pathogen responsible for rice blast disease.

Authors:  K A Parsons; F G Chumley; B Valent
Journal:  Proc Natl Acad Sci U S A       Date:  1987-06       Impact factor: 11.205

8.  Development of a homologous transformation system for Aspergillus niger based on the pyrG gene.

Authors:  W van Hartingsveldt; I E Mattern; C M van Zeijl; P H Pouwels; C A van den Hondel
Journal:  Mol Gen Genet       Date:  1987-01

9.  The isolation and characterization of mutants defective in nitrate assimilation in Neurospora crassa.

Authors:  A B Tomsett; R H Garrett
Journal:  Genetics       Date:  1980-07       Impact factor: 4.562

10.  Cloning and characterization of the gene for beta-tubulin from a benomyl-resistant mutant of Neurospora crassa and its use as a dominant selectable marker.

Authors:  M J Orbach; E B Porro; C Yanofsky
Journal:  Mol Cell Biol       Date:  1986-07       Impact factor: 4.272
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  26 in total

1.  A group of alpha-1,4-glucan lyase genes from the fungi Morchella costata, M. vulgaris and Peziza ostracoderma. Cloning, complete sequencing and heterologous expression.

Authors:  K Bojsen; S Yu; J Marcussen
Journal:  Plant Mol Biol       Date:  1999-06       Impact factor: 4.076

2.  Foret1, a reverse transcriptase-like sequence in the filamentous fungus Fusarium oxysporum.

Authors:  J Julien; S Poirier-Hamon; Y Brygoo
Journal:  Nucleic Acids Res       Date:  1992-08-11       Impact factor: 16.971

3.  Transposition of the autonomous Fot1 element in the filamentous fungus Fusarium oxysporum.

Authors:  Q Migheli; R Laugé; J M Davière; C Gerlinger; F Kaper; T Langin; M J Daboussi
Journal:  Genetics       Date:  1999-03       Impact factor: 4.562

4.  Cloning and expression of a chitinase gene from the hyperparasitic fungus Aphanocladium album.

Authors:  P L Blaiseau; C Kunz; R Grison; Y Bertheau; Y Brygoo
Journal:  Curr Genet       Date:  1992-01       Impact factor: 3.886

5.  Transformation of a methionine auxotrophic mutant of Mucor circinelloides by direct cloning of the corresponding wild type gene.

Authors:  N Anaya; M I Roncero
Journal:  Mol Gen Genet       Date:  1991-12

6.  Molecular analysis of hypervirulent somatic hybrids of the entomopathogenic fungi Beauveria bassiana and Beauveria sulfurescens.

Authors:  M Viaud; Y Couteaudier; G Riba
Journal:  Appl Environ Microbiol       Date:  1998-01       Impact factor: 4.792

7.  Fot 1 insertions in the Fusarium oxysporum f. sp. albedinis genome provide diagnostic PCR targets for detection of the date palm pathogen.

Authors:  D Fernandez; M Ouinten; A Tantaoui; J P Geiger; M J Daboussi; T Langin
Journal:  Appl Environ Microbiol       Date:  1998-02       Impact factor: 4.792

8.  Transformation of the rice blast fungus Magnaporthe grisea to hygromycin B resistance.

Authors:  H Leung; U Lehtinen; R Karjalainen; D Skinner; P Tooley; S Leong; A Ellingboe
Journal:  Curr Genet       Date:  1990-05       Impact factor: 3.886

9.  The GUS gene fusion system (Escherichia coli beta-D-glucuronidase gene), a useful tool in studies of root colonization by Fusarium oxysporum.

Authors:  Y Couteaudier; M J Daboussi; A Eparvier; T Langin; J Orcival
Journal:  Appl Environ Microbiol       Date:  1993-06       Impact factor: 4.792

10.  Transient and stable gene expression in the fungal maize pathogen Cochliobolus heterostrophus after transformation with the beta-glucuronidase (GUS) gene.

Authors:  E Mönke; W Schäfer
Journal:  Mol Gen Genet       Date:  1993-10
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