Literature DB >> 20057072

Cloning, expression and crystallization of dihydrodipicolinate reductase from methicillin-resistant Staphylococcus aureus.

Sudhir Dommaraju1, Michael A Gorman, Con Dogovski, F Grant Pearce, Juliet A Gerrard, Renwick C J Dobson, Michael W Parker, Matthew A Perugini.   

Abstract

Dihydrodipicolinate reductase (DHDPR; EC 1.3.1.26) catalyzes the nucleotide (NADH/NADPH) dependent second step of the lysine-biosynthesis pathway in bacteria and plants. Here, the cloning, expression, purification, crystallization and preliminary X-ray diffraction analysis of DHDPR from methicillin-resistant Staphylococcus aureus (MRSA-DHDPR) are presented. The enzyme was crystallized in a number of forms, predominantly with ammonium sulfate as a precipitant, with the best crystal form diffracting to beyond 3.65 A resolution. Crystal structures of the apo form as well as of cofactor (NADPH) bound and inhibitor (2,6-pyridinedicarboxylate) bound forms of MRSA-DHDPR will provide insight into the structure and function of this essential enzyme and valid drug target.

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Year:  2009        PMID： 20057072      PMCID： PMC2805538          DOI： 10.1107/S1744309109047964

Source DB:  PubMed          Journal:  Acta Crystallogr Sect F Struct Biol Cryst Commun        ISSN： 1744-3091


  18 in total

1.  Essential Bacillus subtilis genes.

Authors:  K Kobayashi; S D Ehrlich; A Albertini; G Amati; K K Andersen; M Arnaud; K Asai; S Ashikaga; S Aymerich; P Bessieres; F Boland; S C Brignell; S Bron; K Bunai; J Chapuis; L C Christiansen; A Danchin; M Débarbouille; E Dervyn; E Deuerling; K Devine; S K Devine; O Dreesen; J Errington; S Fillinger; S J Foster; Y Fujita; A Galizzi; R Gardan; C Eschevins; T Fukushima; K Haga; C R Harwood; M Hecker; D Hosoya; M F Hullo; H Kakeshita; D Karamata; Y Kasahara; F Kawamura; K Koga; P Koski; R Kuwana; D Imamura; M Ishimaru; S Ishikawa; I Ishio; D Le Coq; A Masson; C Mauël; R Meima; R P Mellado; A Moir; S Moriya; E Nagakawa; H Nanamiya; S Nakai; P Nygaard; M Ogura; T Ohanan; M O'Reilly; M O'Rourke; Z Pragai; H M Pooley; G Rapoport; J P Rawlins; L A Rivas; C Rivolta; A Sadaie; Y Sadaie; M Sarvas; T Sato; H H Saxild; E Scanlan; W Schumann; J F M L Seegers; J Sekiguchi; A Sekowska; S J Séror; M Simon; P Stragier; R Studer; H Takamatsu; T Tanaka; M Takeuchi; H B Thomaides; V Vagner; J M van Dijl; K Watabe; A Wipat; H Yamamoto; M Yamamoto; Y Yamamoto; K Yamane; K Yata; K Yoshida; H Yoshikawa; U Zuber; N Ogasawara
Journal:  Proc Natl Acad Sci U S A       Date:  2003-04-07       Impact factor: 11.205

Review 2.  Inhibitors of lysine biosynthesis as antibacterial agents.

Authors:  Craig A Hutton; Timothy J Southwood; Jennifer J Turner
Journal:  Mini Rev Med Chem       Date:  2003-03       Impact factor: 3.862

3.  The structure of dihydrodipicolinate reductase (DapB) from Mycobacterium tuberculosis in three crystal forms.

Authors:  Robert Janowski; Georgia Kefala; Manfred S Weiss
Journal:  Acta Crystallogr D Biol Crystallogr       Date:  2009-12-21

4.  The three-dimensional structures of the Mycobacterium tuberculosis dihydrodipicolinate reductase-NADH-2,6-PDC and -NADPH-2,6-PDC complexes. Structural and mutagenic analysis of relaxed nucleotide specificity.

Authors:  Maurizio Cirilli; Renjian Zheng; Giovanna Scapin; John S Blanchard
Journal:  Biochemistry       Date:  2003-09-16       Impact factor: 3.162

5.  Purification, crystallization and preliminary X-ray diffraction studies to near-atomic resolution of dihydrodipicolinate synthase from methicillin-resistant Staphylococcus aureus.

Authors:  Benjamin R Burgess; Renwick C J Dobson; Con Dogovski; Geoffrey B Jameson; Michael W Parker; Matthew A Perugini
Journal:  Acta Crystallogr Sect F Struct Biol Cryst Commun       Date:  2008-06-28

Review 6.  Waves of resistance: Staphylococcus aureus in the antibiotic era.

Authors:  Henry F Chambers; Frank R Deleo
Journal:  Nat Rev Microbiol       Date:  2009-09       Impact factor: 60.633

7.  The crystal structure of three site-directed mutants of Escherichia coli dihydrodipicolinate synthase: further evidence for a catalytic triad.

Authors:  Renwick C J Dobson; Karin Valegård; Juliet A Gerrard
Journal:  J Mol Biol       Date:  2004-04-23       Impact factor: 5.469

8.  Evolution of quaternary structure in a homotetrameric enzyme.

Authors:  Michael D W Griffin; Renwick C J Dobson; F Grant Pearce; Laurence Antonio; Andrew E Whitten; Chu K Liew; Joel P Mackay; Jill Trewhella; Geoffrey B Jameson; Matthew A Perugini; Juliet A Gerrard
Journal:  J Mol Biol       Date:  2008-05-22       Impact factor: 5.469

9.  Crystallization and preliminary X-ray analysis of dihydrodipicolinate synthase from Clostridium botulinum in the presence of its substrate pyruvate.

Authors:  Sarah C Atkinson; Renwick C J Dobson; Janet M Newman; Michael A Gorman; Con Dogovski; Michael W Parker; Matthew A Perugini
Journal:  Acta Crystallogr Sect F Struct Biol Cryst Commun       Date:  2009-02-14

10.  Expression, purification, crystallization and preliminary X-ray diffraction analysis of dihydrodipicolinate synthase from Bacillus anthracis in the presence of pyruvate.

Authors:  Jarrod E Voss; Stephen W Scally; Nicole L Taylor; Con Dogovski; Malcolm R Alderton; Craig A Hutton; Juliet A Gerrard; Michael W Parker; Renwick C J Dobson; Matthew A Perugini
Journal:  Acta Crystallogr Sect F Struct Biol Cryst Commun       Date:  2009-01-31
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  6 in total

1.  Cloning, expression, purification and crystallization of dihydrodipicolinate synthase from the grapevine Vitis vinifera.

Authors:  Sarah C Atkinson; Con Dogovski; Janet Newman; Renwick C J Dobson; Matthew A Perugini
Journal:  Acta Crystallogr Sect F Struct Biol Cryst Commun       Date:  2011-11-25

2.  Cloning, expression, purification and crystallization of dihydrodipicolinate synthase from the psychrophile Shewanella benthica.

Authors:  Jacinta M Wubben; Con Dogovski; Renwick C J Dobson; Rachel Codd; Juliet A Gerrard; Michael W Parker; Matthew A Perugini
Journal:  Acta Crystallogr Sect F Struct Biol Cryst Commun       Date:  2010-10-29

3.  Cloning, expression, purification and crystallization of dihydrodipicolinate synthase from Agrobacterium tumefaciens.

Authors:  Sarah C Atkinson; Con Dogovski; Renwick C J Dobson; Matthew A Perugini
Journal:  Acta Crystallogr Sect F Struct Biol Cryst Commun       Date:  2012-08-30

Review 4.  Molecular evolution of an oligomeric biocatalyst functioning in lysine biosynthesis.

Authors:  Tatiana P Soares da Costa; Belinda M Abbott; Anthony R Gendall; Santosh Panjikar; Matthew A Perugini
Journal:  Biophys Rev       Date:  2017-12-05

5.  Structure and Function of Cyanobacterial DHDPS and DHDPR.

Authors:  Janni B Christensen; T P Soares da Costa; Pierre Faou; F Grant Pearce; Santosh Panjikar; Matthew A Perugini
Journal:  Sci Rep       Date:  2016-11-15       Impact factor: 4.379

6.  Characterisation of the first enzymes committed to lysine biosynthesis in Arabidopsis thaliana.

Authors:  Michael D W Griffin; Jagan M Billakanti; Akshita Wason; Sabrina Keller; Haydyn D T Mertens; Sarah C Atkinson; Renwick C J Dobson; Matthew A Perugini; Juliet A Gerrard; Frederick Grant Pearce
Journal:  PLoS One       Date:  2012-07-05       Impact factor: 3.240
  6 in total

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