Literature DB >> 19929015

Double-nucleus enhanced recoupling for efficient 13C MAS NMR correlation spectroscopy of perdeuterated proteins.

Umit Akbey1, Hartmut Oschkinat, Barth-Jan van Rossum.   

Abstract

The use of both proton and deuterium dipolar coupling networks to obtain efficient (13)C magic-angle-spinning NMR correlation spectroscopy is introduced. This new strategy aims to improve the efficiency of (13)C spin diffusion in perdeuterated proteins. The method is called double-nucleus enhanced recoupling (DONER), and it provides significantly improved transfer efficiency for carbon spin diffusion at low proton density. The C(alpha) cross-peak intensity in the DONER experiment is approximately 3 and approximately 5 times stronger than those in conventional radio-frequency-assisted diffusion (RAD) and proton-driven spin diffusion (PDSD) experiments, respectively. Remarkably, the full cross-peak pattern for the aliphatic region of an extensively perdeuterated SH3 protein sample can be obtained using the DONER approach with direct (13)C excitation.

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Year:  2009        PMID： 19929015     DOI： 10.1021/ja907493p

Source DB:  PubMed          Journal:  J Am Chem Soc        ISSN： 0002-7863            Impact factor:   15.419


  9 in total

1.  Three-dimensional deuterium-carbon correlation experiments for high-resolution solid-state MAS NMR spectroscopy of large proteins.

Authors:  Daniela Lalli; Paul Schanda; Anup Chowdhury; Joren Retel; Matthias Hiller; Victoria A Higman; Lieselotte Handel; Vipin Agarwal; Bernd Reif; Barth van Rossum; Umit Akbey; Hartmut Oschkinat
Journal:  J Biomol NMR       Date:  2011-10-25       Impact factor: 2.835

2.  Backbone assignment of perdeuterated proteins using long-range H/C-dipolar transfers.

Authors:  Rasmus Linser
Journal:  J Biomol NMR       Date:  2011-12-14       Impact factor: 2.835

3.  Dynamic nuclear polarization of deuterated proteins.

Authors:  Ümit Akbey; W Trent Franks; Arne Linden; Sascha Lange; Robert G Griffin; Barth-Jan van Rossum; Hartmut Oschkinat
Journal:  Angew Chem Int Ed Engl       Date:  2010-10-11       Impact factor: 15.336

4.  Asynchronous through-bond homonuclear isotropic mixing: application to carbon-carbon transfer in perdeuterated proteins under MAS.

Authors:  Natalia Kulminskaya; Suresh Kumar Vasa; Karin Giller; Stefan Becker; Rasmus Linser
Journal:  J Biomol NMR       Date:  2015-08-30       Impact factor: 2.835

5.  Homonuclear mixing sequences for perdeuterated proteins.

Authors:  Kuo-Ying Huang; Ansgar B Siemer; Ann E McDermott
Journal:  J Magn Reson       Date:  2010-10-26       Impact factor: 2.229

6.  Cryogenic temperature effects and resolution upon slow cooling of protein preparations in solid state NMR.

Authors:  Arne H Linden; W Trent Franks; Ümit Akbey; Sascha Lange; Barth-Jan van Rossum; Hartmut Oschkinat
Journal:  J Biomol NMR       Date:  2011-08-09       Impact factor: 2.835

7.  Side-chain to backbone correlations from solid-state NMR of perdeuterated proteins through combined excitation and long-range magnetization transfers.

Authors:  Rasmus Linser
Journal:  J Biomol NMR       Date:  2011-08-07       Impact factor: 2.835

8.  Design and construction of a quadruple-resonance MAS NMR probe for investigation of extensively deuterated biomolecules.

Authors:  Kelsey A Collier; Suvrajit Sengupta; Catalina A Espinosa; John E Kelly; Jessica I Kelz; Rachel W Martin
Journal:  J Magn Reson       Date:  2017-10-12       Impact factor: 2.229

9.  Transferred-Rotational-Echo Double Resonance.

Authors:  Xizhou Cecily Zhang; Marcel C Forster; Evgeny Nimerovsky; Kumar Tekwani Movellan; Loren B Andreas
Journal:  J Phys Chem A       Date:  2021-01-19       Impact factor: 2.781
  9 in total

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