Literature DB >> 19821611

IspG enzyme activity in the deoxyxylulose phosphate pathway: roles of the iron-sulfur cluster.

Youli Xiao1, Georgia Zahariou, Yiannis Sanakis, Pinghua Liu.   

Abstract

IspG is a [4Fe-4S] cluster-containing protein, and the [4Fe-4S](+) species is proposed to be the catalytically relevant species. However, attempts reported in the literature failed to detect the [4Fe-4S](+) species. In this study, using a potent reduction system, we have successfully detected the [4Fe-4S](+) species with X-band EPR spectroscopy. In addition, we have improved the Escherichia coli IspG activity to 550 nmol min(-1) mg(-1), which is approximately 20-fold greater than that of the NADPH-Fpr-FldA system in the literature.

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Year:  2009        PMID： 19821611     DOI： 10.1021/bi901519q

Source DB:  PubMed          Journal:  Biochemistry        ISSN： 0006-2960            Impact factor:   3.162


  12 in total

1.  Uncovering the functional residues of Arabidopsis isoprenoid biosynthesis enzyme HDS.

Authors:  Jin-Zheng Wang; Yongxing Lei; Yanmei Xiao; Xiang He; Jiubo Liang; Jishan Jiang; Shangzhi Dong; Haiyan Ke; Patricia Leon; Philipp Zerbe; Youli Xiao; Katayoon Dehesh
Journal:  Proc Natl Acad Sci U S A       Date:  2019-12-26       Impact factor: 11.205

2.  Engineering a functional 1-deoxy-D-xylulose 5-phosphate (DXP) pathway in Saccharomyces cerevisiae.

Authors:  James Kirby; Kevin L Dietzel; Gale Wichmann; Rossana Chan; Eugene Antipov; Nathan Moss; Edward E K Baidoo; Peter Jackson; Sara P Gaucher; Shayin Gottlieb; Jeremy LaBarge; Tina Mahatdejkul; Kristy M Hawkins; Sheela Muley; Jack D Newman; Pinghua Liu; Jay D Keasling; Lishan Zhao
Journal:  Metab Eng       Date:  2016-10-27       Impact factor: 9.783

Review 3.  Biochemistry of the non-mevalonate isoprenoid pathway.

Authors:  Tobias Gräwert; Michael Groll; Felix Rohdich; Adelbert Bacher; Wolfgang Eisenreich
Journal:  Cell Mol Life Sci       Date:  2011-07-09       Impact factor: 9.261

Review 4.  Methylerythritol phosphate pathway of isoprenoid biosynthesis.

Authors:  Lishan Zhao; Wei-chen Chang; Youli Xiao; Hung-wen Liu; Pinghua Liu
Journal:  Annu Rev Biochem       Date:  2013       Impact factor: 23.643

5.  IspG-catalyzed positional isotopic exchange in methylerythritol cyclodiphosphate of the deoxyxylulose phosphate pathway: mechanistic implications.

Authors:  Youli Xiao; Debra Rooker; Quincy You; Caren L Freel Meyers; Pinghua Liu
Journal:  Chembiochem       Date:  2011-01-26       Impact factor: 3.164

6.  Methylerythritol cyclodiphosphate (MEcPP) in deoxyxylulose phosphate pathway: synthesis from an epoxide and mechanisms.

Authors:  Youli Xiao; Rodney L Nyland; Caren L Freel Meyers; Pinghua Liu
Journal:  Chem Commun (Camb)       Date:  2010-09-01       Impact factor: 6.222

Review 7.  MICROBIAL isoprene production: an overview.

Authors:  Jasmine Isar; Dharmendra Jain; Harshvardhan Joshi; Shrikant Dhoot; Vidhya Rangaswamy
Journal:  World J Microbiol Biotechnol       Date:  2022-05-31       Impact factor: 3.312

Review 8.  Current development in isoprenoid precursor biosynthesis and regulation.

Authors:  Wei-chen Chang; Heng Song; Hung-wen Liu; Pinghua Liu
Journal:  Curr Opin Chem Biol       Date:  2013-07-24       Impact factor: 8.822

9.  IspG converts an epoxide substrate analogue to (E)-4-hydroxy-3-methylbut-2-enyl diphosphate: implications for IspG catalysis in isoprenoid biosynthesis.

Authors:  Rodney L Nyland; Youli Xiao; Pinghua Liu; Caren L Freel Meyers
Journal:  J Am Chem Soc       Date:  2009-12-16       Impact factor: 15.419

Review 10.  Isoprenoid precursor biosynthesis offers potential targets for drug discovery against diseases caused by apicomplexan parasites.

Authors:  William N Hunter
Journal:  Curr Top Med Chem       Date:  2011       Impact factor: 3.295
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