Literature DB >> 19710270

Comparative evaluation of a commercially available automated system for extraction of viral DNA from whole blood: application to monitoring of epstein-barr virus and cytomegalovirus load.

Sylvie Pillet1, Thomas Bourlet, Bruno Pozzetto.   

Abstract

The NucliSENS easyMAG automated system was compared to the column-based Qiagen method for Epstein-Barr virus (EBV) or cytomegalovirus (CMV) DNA extraction from whole blood before viral load determination using the corresponding R-gene amplification kits. Both extraction techniques exhibited a total agreement of 81.3% for EBV and 87.2% for CMV.

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Year:  2009        PMID： 19710270      PMCID： PMC2772604          DOI： 10.1128/JCM.01497-09

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


  20 in total

1.  Quantitative analysis of HCMV DNA load in whole blood of renal transplant patients using real-time PCR assay.

Authors:  S Gouarin; A Vabret; E Gault; J Petitjean; A Regeasse; B Hurault de Ligny; F Freymuth
Journal:  J Clin Virol       Date:  2004-03       Impact factor: 3.168

2.  Rapid and simple method for purification of nucleic acids.

Authors:  R Boom; C J Sol; M M Salimans; C L Jansen; P M Wertheim-van Dillen; J van der Noordaa
Journal:  J Clin Microbiol       Date:  1990-03       Impact factor: 5.948

3.  Whole blood real-time quantitative PCR for cytomegalovirus infection follow-up in transplant recipients.

Authors:  Isabelle Garrigue; Sébastien Boucher; Lionel Couzi; Anne Caumont; Claire Dromer; Martine Neau-Cransac; Reza Tabrizi; Marie-Hélène Schrive; Hervé Fleury; Marie-Edith Lafon
Journal:  J Clin Virol       Date:  2006-02-14       Impact factor: 3.168

4.  Automated extraction of viral-pathogen RNA and DNA for high-throughput quantitative real-time PCR.

Authors:  Kurt Beuselinck; Marc van Ranst; J van Eldere
Journal:  J Clin Microbiol       Date:  2005-11       Impact factor: 5.948

Review 5.  Epstein-Barr virus load monitoring: its role in the prevention and management of post-transplant lymphoproliferative disease.

Authors:  D T Rowe; S Webber; E M Schauer; J Reyes; M Green
Journal:  Transpl Infect Dis       Date:  2001-06       Impact factor: 2.228

6.  Longitudinal analysis of Epstein-Barr viral load in plasma and peripheral blood mononuclear cells of transplanted patients by real-time polymerase chain reaction.

Authors:  Hans-Joachim Wagner; Lars Fischer; Wolfram J Jabs; Meike Holbe; Klaus Pethig; Peter Bucsky
Journal:  Transplantation       Date:  2002-09-15       Impact factor: 4.939

7.  Measurement of Epstein-Barr virus DNA loads in whole blood and plasma by TaqMan PCR and in peripheral blood lymphocytes by competitive PCR.

Authors:  Robert M Wadowsky; Stella Laus; Michael Green; Steven A Webber; David Rowe
Journal:  J Clin Microbiol       Date:  2003-11       Impact factor: 5.948

8.  Cellular normalization of viral DNA loads on whole blood improves the clinical management of cytomegalovirus or Epstein Barr virus infections in the setting of pre-emptive therapy.

Authors:  Céline Bressollette-Bodin; Marianne Coste-Burel; Bernard Besse; Elisabeth André-Garnier; Virginie Ferre; Berthe-Marie Imbert-Marcille
Journal:  J Med Virol       Date:  2009-01       Impact factor: 2.327

9.  Assessment of automated DNA extraction coupled with real-time PCR for measuring Epstein-Barr virus load in whole blood, peripheral mononuclear cells and plasma.

Authors:  Samira Fafi-Kremer; Karen Brengel-Pesce; Gérard Barguès; Marie-Josette Bourgeat; Odile Genoulaz; Jean-Marie Seigneurin; Patrice Morand
Journal:  J Clin Virol       Date:  2004-06       Impact factor: 3.168

10.  Automated extraction and quantification of human cytomegalovirus DNA in whole blood by real-time PCR assay.

Authors:  C Mengelle; K Sandres-Sauné; C Pasquier; L Rostaing; J-M Mansuy; M Marty; I Da Silva; M Attal; P Massip; J Izopet
Journal:  J Clin Microbiol       Date:  2003-08       Impact factor: 5.948
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  7 in total

1.  Comparison of QIAsymphony automated and QIAamp manual DNA extraction systems for measuring Epstein-Barr virus DNA load in whole blood using real-time PCR.

Authors:  Stella Laus; Lawrence A Kingsley; Michael Green; Robert M Wadowsky
Journal:  J Mol Diagn       Date:  2011-09-01       Impact factor: 5.568

2.  Optimization of Toxoplasma gondii DNA extraction from amniotic fluid using NucliSENS easyMAG and comparison with QIAamp DNA minikit.

Authors:  B Faucher; F Miermont; S Ranque; J Franck; R Piarroux
Journal:  Eur J Clin Microbiol Infect Dis       Date:  2011-09-04       Impact factor: 3.267

3.  Comparative evaluation of three automated systems for DNA extraction in conjunction with three commercially available real-time PCR assays for quantitation of plasma Cytomegalovirus DNAemia in allogeneic stem cell transplant recipients.

Authors:  Dayana Bravo; María Ángeles Clari; Elisa Costa; Beatriz Muñoz-Cobo; Carlos Solano; María José Remigia; David Navarro
Journal:  J Clin Microbiol       Date:  2011-06-22       Impact factor: 5.948

4.  Measurement of Epstein-Barr virus DNA load using a novel quantification standard containing two EBV DNA targets and SYBR Green I dye.

Authors:  Meav-Lang J Lay; Robyn M Lucas; Mala Ratnamohan; Janette Taylor; Anne-Louise Ponsonby; Dominic E Dwyer
Journal:  Virol J       Date:  2010-09-22       Impact factor: 4.099

5.  Comparison of eMAG™ versus NucliSENS® EasyMAG® performance on clinical specimens.

Authors:  Magali Garcia; Céline Chessa; Anne Bourgoin; Geneviève Giraudeau; Chloé Plouzeau; Gérard Agius; Nicolas Lévêque; Agnès Beby-Defaux
Journal:  J Clin Virol       Date:  2017-01-29       Impact factor: 3.168

6.  Prospective evaluation of a new automated nucleic acid extraction system using routine clinical respiratory specimens.

Authors:  C Mengelle; J-M Mansuy; K Sandres-Sauné; C Barthe; J Boineau; J Izopet
Journal:  J Med Virol       Date:  2012-06       Impact factor: 2.327

7.  Comparative evaluation of the QIAsymphony RGQ system with the easyMAG/R-gene combination for the quantitation of cytomegalovirus DNA load in whole blood.

Authors:  Sylvie Pillet; Thomas Bourlet; Bruno Pozzetto
Journal:  Virol J       Date:  2012-10-09       Impact factor: 4.099
  7 in total

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