Literature DB >> 14605174

Measurement of Epstein-Barr virus DNA loads in whole blood and plasma by TaqMan PCR and in peripheral blood lymphocytes by competitive PCR.

Robert M Wadowsky1, Stella Laus, Michael Green, Steven A Webber, David Rowe.   

Abstract

Epstein-Barr virus (EBV) DNA load values were measured in samples of whole blood (n = 60) and plasma (n = 59) by TaqMan PCR and in samples of peripheral blood lymphocytes (PBLs) (n = 60) by competitive PCR (cPCR). The samples were obtained from 44 transplant recipients. The whole-blood and PBL loads correlated highly (r(2) > 0.900), whereas the plasma and PBL loads correlated poorly (r(2) = 0.512). Testing of whole blood by TaqMan PCR is an acceptable alternative to testing of PBLs by cPCR for quantifying EBV DNA load.

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Year:  2003        PMID： 14605174      PMCID： PMC262487          DOI： 10.1128/JCM.41.11.5245-5249.2003

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


  11 in total

1.  Quantitative analysis of cell-free Epstein-Barr virus DNA in plasma of patients with nasopharyngeal carcinoma.

Authors:  Y M Lo; L Y Chan; K W Lo; S F Leung; J Zhang; A T Chan; J C Lee; N M Hjelm; P J Johnson; D P Huang
Journal:  Cancer Res       Date:  1999-03-15       Impact factor: 12.701

2.  Analytic validation of a competitive polymerase chain reaction assay for measuring Epstein-Barr viral load.

Authors:  H Fan; S A Schichman; L J Swinnen; J M Nicholls; P A Eagan; M Luther; M L Gulley
Journal:  Diagn Mol Pathol       Date:  2001-12

3.  Comparison of quantitative competitive PCR with LightCycler-based PCR for measuring Epstein-Barr virus DNA load in clinical specimens.

Authors:  Servi J C Stevens; Sandra A W M Verkuijlen; Adriaan J C van den Brule; Jaap M Middeldorp
Journal:  J Clin Microbiol       Date:  2002-11       Impact factor: 5.948

4.  Use of quantitative competitive PCR to measure Epstein-Barr virus genome load in the peripheral blood of pediatric transplant patients with lymphoproliferative disorders.

Authors:  D T Rowe; L Qu; J Reyes; N Jabbour; E Yunis; P Putnam; S Todo; M Green
Journal:  J Clin Microbiol       Date:  1997-06       Impact factor: 5.948

5.  Predictive negative value of persistent low Epstein-Barr virus viral load after intestinal transplantation in children.

Authors:  M Green; J Bueno; D Rowe; G Mazariegos; L Qu; K Abu-Almagd; J Reyes
Journal:  Transplantation       Date:  2000-08-27       Impact factor: 4.939

6.  Use of real-time PCR to measure Epstein-Barr virus genomes in whole blood.

Authors:  E Leung; B K Shenton; G Jackson; F K Gould; C Yap; D Talbot
Journal:  J Immunol Methods       Date:  2002-12-15       Impact factor: 2.303

7.  Detection of Epstein-Barr virus DNA in sera from transplant recipients with lymphoproliferative disorders.

Authors:  A P Limaye; M L Huang; E E Atienza; J M Ferrenberg; L Corey
Journal:  J Clin Microbiol       Date:  1999-04       Impact factor: 5.948

8.  Toward standardization of Epstein-Barr virus DNA load monitoring: unfractionated whole blood as preferred clinical specimen.

Authors:  S J Stevens; I Pronk; J M Middeldorp
Journal:  J Clin Microbiol       Date:  2001-04       Impact factor: 5.948

9.  Serial measurement of Epstein-Barr viral load in peripheral blood in pediatric liver transplant recipients during treatment for posttransplant lymphoproliferative disease.

Authors:  M Green; T V Cacciarelli; G V Mazariegos; L Sigurdsson; L Qu; D T Rowe; J Reyes
Journal:  Transplantation       Date:  1998-12-27       Impact factor: 4.939

10.  Quantitative analysis of Epstein-Barr virus load by using a real-time PCR assay.

Authors:  H Kimura; M Morita; Y Yabuta; K Kuzushima; K Kato; S Kojima; T Matsuyama; T Morishima
Journal:  J Clin Microbiol       Date:  1999-01       Impact factor: 5.948
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  28 in total

Review 1.  Real-time PCR in clinical microbiology: applications for routine laboratory testing.

Authors:  M J Espy; J R Uhl; L M Sloan; S P Buckwalter; M F Jones; E A Vetter; J D C Yao; N L Wengenack; J E Rosenblatt; F R Cockerill; T F Smith
Journal:  Clin Microbiol Rev       Date:  2006-01       Impact factor: 26.132

2.  Comparative evaluation of a commercially available automated system for extraction of viral DNA from whole blood: application to monitoring of epstein-barr virus and cytomegalovirus load.

Authors:  Sylvie Pillet; Thomas Bourlet; Bruno Pozzetto
Journal:  J Clin Microbiol       Date:  2009-08-26       Impact factor: 5.948

3.  Comparison of QIAsymphony automated and QIAamp manual DNA extraction systems for measuring Epstein-Barr virus DNA load in whole blood using real-time PCR.

Authors:  Stella Laus; Lawrence A Kingsley; Michael Green; Robert M Wadowsky
Journal:  J Mol Diagn       Date:  2011-09-01       Impact factor: 5.568

Review 4.  Viral surveillance and subclinical viral infection in pediatric kidney transplantation.

Authors:  Jodi M Smith; Vikas R Dharnidharka
Journal:  Pediatr Nephrol       Date:  2014-08-16       Impact factor: 3.714

5.  Diagnostic value of measuring Epstein-Barr virus (EBV) DNA load and carcinoma-specific viral mRNA in relation to anti-EBV immunoglobulin A (IgA) and IgG antibody levels in blood of nasopharyngeal carcinoma patients from Indonesia.

Authors:  Servi J C Stevens; Sandra A W M Verkuijlen; Bambang Hariwiyanto; Jajah Fachiroh; Dewi K Paramita; I Bing Tan; Sophia M Haryana; Jaap M Middeldorp
Journal:  J Clin Microbiol       Date:  2005-07       Impact factor: 5.948

6.  Subclinical herpesvirus shedding among HIV-1-infected men on antiretroviral therapy.

Authors:  Arcadio Agudelo-Hernandez; Yue Chen; Arlene Bullotta; William G Buchanan; Cynthia R Klamar-Blain; Luann Borowski; Sharon A Riddler; Charles R Rinaldo; Bernard J C Macatangay
Journal:  AIDS       Date:  2017-09-24       Impact factor: 4.177

7.  Multicenter comparison of different real-time PCR assays for quantitative detection of Epstein-Barr virus.

Authors:  R T Hayden; K M Hokanson; S B Pounds; M J Bankowski; S W Belzer; J Carr; D Diorio; M S Forman; Y Joshi; D Hillyard; R L Hodinka; M N Nikiforova; C A Romain; J Stevenson; A Valsamakis; H H Balfour
Journal:  J Clin Microbiol       Date:  2007-11-07       Impact factor: 5.948

8.  Treatment-dependent loss of polyfunctional CD8+ T-cell responses in HIV-infected kidney transplant recipients is associated with herpesvirus reactivation.

Authors:  O Gasser; F Bihl; S Sanghavi; C Rinaldo; D Rowe; C Hess; D Stablein; M Roland; P Stock; C Brander
Journal:  Am J Transplant       Date:  2009-04       Impact factor: 8.086

9.  Comparison of various blood compartments and reporting units for the detection and quantification of Epstein-Barr virus in peripheral blood.

Authors:  H Hakim; C Gibson; J Pan; K Srivastava; Z Gu; M J Bankowski; R T Hayden
Journal:  J Clin Microbiol       Date:  2007-05-09       Impact factor: 5.948

10.  Epstein-Barr virus: general factors, virus-related diseases and measurement of viral load after transplant.

Authors:  Luciana Cristina Fagundes Gequelin; Irina N Riediger; Sueli M Nakatani; Alexander W Biondo; Carmem M Bonfim
Journal:  Rev Bras Hematol Hemoter       Date:  2011
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