Literature DB >> 19458181

Rapid identification of biothreat and other clinically relevant bacterial species by use of universal PCR coupled with high-resolution melting analysis.

Samuel Yang1, Padmini Ramachandran, Richard Rothman, Yu-Hsiang Hsieh, Andrew Hardick, Helen Won, Aleksandar Kecojevic, Joany Jackman, Charlotte Gaydos.   

Abstract

A rapid assay for eubacterial species identification is described using high-resolution melt analysis to characterize PCR products. Unique melt profiles generated from multiple hypervariable regions of the 16S rRNA gene for 100 clinically relevant bacterial pathogens, including category A and B biothreat agents and their surrogates, allowed highly specific species identification.

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Year:  2009        PMID： 19458181      PMCID： PMC2708498          DOI： 10.1128/JCM.00033-09

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


  14 in total

Review 1.  Mass casualty management of a large-scale bioterrorist event: an epidemiological approach that shapes triage decisions.

Authors:  Frederick M Burkle
Journal:  Emerg Med Clin North Am       Date:  2002-05       Impact factor: 2.264

2.  Quantitative multiprobe PCR assay for simultaneous detection and identification to species level of bacterial pathogens.

Authors:  Samuel Yang; Shin Lin; Gabor D Kelen; Thomas C Quinn; James D Dick; Charlotte A Gaydos; Richard E Rothman
Journal:  J Clin Microbiol       Date:  2002-09       Impact factor: 5.948

3.  Advances in detecting and responding to threats from bioterrorism and emerging infectious disease.

Authors:  Lisa D Rotz; James M Hughes
Journal:  Nat Med       Date:  2004-12       Impact factor: 53.440

Review 4.  Current and developing technologies for monitoring agents of bioterrorism and biowarfare.

Authors:  Daniel V Lim; Joyce M Simpson; Elizabeth A Kearns; Marianne F Kramer
Journal:  Clin Microbiol Rev       Date:  2005-10       Impact factor: 26.132

Review 5.  Impact of 16S rRNA gene sequence analysis for identification of bacteria on clinical microbiology and infectious diseases.

Authors:  Jill E Clarridge
Journal:  Clin Microbiol Rev       Date:  2004-10       Impact factor: 26.132

6.  A 768-lane microfabricated system for high-throughput DNA sequencing.

Authors:  James H Aborn; Sameh A El-Difrawy; Mark Novotny; Elizabeth A Gismondi; Roger Lam; Paul Matsudaira; Brian K McKenna; Thomas O'Neil; Philip Streechon; Daniel J Ehrlich
Journal:  Lab Chip       Date:  2005-05-09       Impact factor: 6.799

7.  Rapid identification and strain-typing of respiratory pathogens for epidemic surveillance.

Authors:  David J Ecker; Rangarajan Sampath; Lawrence B Blyn; Mark W Eshoo; Cristina Ivy; Joseph A Ecker; Brian Libby; Vivek Samant; Kristin A Sannes-Lowery; Rachael E Melton; Kevin Russell; Nikki Freed; Chris Barrozo; Jianguo Wu; Karl Rudnick; Anjali Desai; Emily Moradi; Duane J Knize; David W Robbins; James C Hannis; Patina M Harrell; Christian Massire; Thomas A Hall; Yun Jiang; Raymond Ranken; Jared J Drader; Neill White; John A McNeil; Stanley T Crooke; Steven A Hofstadler
Journal:  Proc Natl Acad Sci U S A       Date:  2005-05-23       Impact factor: 11.205

8.  High-resolution genotyping by amplicon melting analysis using LCGreen.

Authors:  Carl T Wittwer; Gudrun H Reed; Cameron N Gundry; Joshua G Vandersteen; Robert J Pryor
Journal:  Clin Chem       Date:  2003-06       Impact factor: 8.327

9.  Rapid PCR-based diagnosis of septic arthritis by early Gram-type classification and pathogen identification.

Authors:  Samuel Yang; Padmini Ramachandran; Andrew Hardick; Yu-Hsiang Hsieh; Celeste Quianzon; Marcos Kuroki; Justin Hardick; Aleksandar Kecojevic; Avanthi Abeygunawardena; Jonathan Zenilman; Johan Melendez; Vishal Doshi; Charlotte Gaydos; Richard E Rothman
Journal:  J Clin Microbiol       Date:  2008-02-27       Impact factor: 5.948

Review 10.  PCR-based diagnostics for infectious diseases: uses, limitations, and future applications in acute-care settings.

Authors:  Samuel Yang; Richard E Rothman
Journal:  Lancet Infect Dis       Date:  2004-06       Impact factor: 25.071
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  32 in total

1.  Rapid identification of bacterial pathogens in positive blood culture bottles by use of a broad-based PCR assay coupled with high-resolution melt analysis.

Authors:  Helen Won; Richard Rothman; Padmini Ramachandran; Yu-Hsiang Hsieh; Aleksandar Kecojevic; Karen C Carroll; Deborah Aird; Charlotte Gaydos; Samuel Yang
Journal:  J Clin Microbiol       Date:  2010-07-14       Impact factor: 5.948

2.  Use of quantitative broad-based polymerase chain reaction for detection and identification of common bacterial pathogens in cerebrospinal fluid.

Authors:  Richard Rothman; Padmini Ramachandran; Samuel Yang; Andrew Hardick; Helen Won; Aleksandar Kecojevic; Celeste Quianzon; Yu-Hsiang Hsieh; Charlotte Gaydos
Journal:  Acad Emerg Med       Date:  2010-07       Impact factor: 3.451

3.  Usefulness of PCR-HRMA in identification of non-fermentative Gram-negative rods recovered from patients suffering from cystic fibrosis or chronic obstructive pulmonary disease.

Authors:  Lucie Navrátilová; Dana Safářová; Vladislav Raclavský
Journal:  Folia Microbiol (Praha)       Date:  2013-06-13       Impact factor: 2.099

4.  Rapid detection and statistical differentiation of KPC gene variants in Gram-negative pathogens by use of high-resolution melting and ScreenClust analyses.

Authors:  Amanda L Roth; Nancy D Hanson
Journal:  J Clin Microbiol       Date:  2012-10-17       Impact factor: 5.948

Review 5.  Microbiological applications of high-resolution melting analysis.

Authors:  Steven Y C Tong; Philip M Giffard
Journal:  J Clin Microbiol       Date:  2012-08-08       Impact factor: 5.948

6.  Comparative analysis of two broad-range PCR assays for pathogen detection in positive-blood-culture bottles: PCR-high-resolution melting analysis versus PCR-mass spectrometry.

Authors:  Kevin Jeng; Charlotte A Gaydos; Lawrence B Blyn; Samuel Yang; Helen Won; Heather Matthews; Donna Toleno; Yu-Hsiang Hsieh; Karen C Carroll; Justin Hardick; Billy Masek; Alexander Kecojevic; Rangarajan Sampath; Stephen Peterson; Richard E Rothman
Journal:  J Clin Microbiol       Date:  2012-08-01       Impact factor: 5.948

7.  A broad range assay for rapid detection and etiologic characterization of bacterial meningitis: performance testing in samples from sub-Sahara.

Authors:  Helen Won; Samuel Yang; Charlotte Gaydos; Justin Hardick; Padmini Ramachandran; Yu-Hsiang Hsieh; Alexander Kecojevic; Berthe-Marie Njanpop-Lafourcade; Judith E Mueller; Tsidi Agbeko Tameklo; Kossi Badziklou; Bradford D Gessner; Richard E Rothman
Journal:  Diagn Microbiol Infect Dis       Date:  2012-07-17       Impact factor: 2.803

8.  Multiplex high-resolution melting analysis as a diagnostic tool for detection of plasmid-mediated AmpC β-lactamase genes.

Authors:  Chelsie N Geyer; Nancy D Hanson
Journal:  J Clin Microbiol       Date:  2014-01-29       Impact factor: 5.948

9.  Application of a 16S rRNA PCR-high-resolution melt analysis assay for rapid detection of Salmonella Bacteremia.

Authors:  Kevin Jeng; Samuel Yang; Helen Won; Charlotte A Gaydos; Yu-Hsiang Hsieh; Alex Kecojevic; Karen C Carroll; Justin Hardick; Richard E Rothman
Journal:  J Clin Microbiol       Date:  2012-01-11       Impact factor: 5.948

10.  Sensitive detection and serovar differentiation of typhoidal and nontyphoidal Salmonella enterica species using 16S rRNA Gene PCR coupled with high-resolution melt analysis.

Authors:  Billie J Masek; Justin Hardick; Helen Won; Samuel Yang; Yu-Hsiang Hsieh; Richard E Rothman; Charlotte A Gaydos
Journal:  J Mol Diagn       Date:  2013-12-21       Impact factor: 5.568
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