Literature DB >> 20631110

Rapid identification of bacterial pathogens in positive blood culture bottles by use of a broad-based PCR assay coupled with high-resolution melt analysis.

Helen Won1, Richard Rothman, Padmini Ramachandran, Yu-Hsiang Hsieh, Aleksandar Kecojevic, Karen C Carroll, Deborah Aird, Charlotte Gaydos, Samuel Yang.   

Abstract

We evaluated a broad-based PCR assay coupled with high-resolution melt analysis for rapid bacterial identification in patients with bacterial sepsis. With a reference library of 60 clinically relevant bacterial species, 52 positive blood culture samples were tested. Our assay identified 46/52 samples at the species level, with 100% concordance to culture findings.

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Year:  2010        PMID： 20631110      PMCID： PMC2937718          DOI： 10.1128/JCM.00718-10

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


  12 in total

1.  Rapid identification of bacteria from positive blood cultures by fluorescence-based PCR-single-strand conformation polymorphism analysis of the 16S rRNA gene.

Authors:  C Y Turenne; E Witwicki; D J Hoban; J A Karlowsky; A M Kabani
Journal:  J Clin Microbiol       Date:  2000-02       Impact factor: 5.948

2.  Rapid identification of bacteria in blood cultures by using fluorescently labeled oligonucleotide probes.

Authors:  G J Jansen; M Mooibroek; J Idema; H J Harmsen; G W Welling; J E Degener
Journal:  J Clin Microbiol       Date:  2000-02       Impact factor: 5.948

Review 3.  Epidemiology of sepsis: an update.

Authors:  D C Angus; R S Wax
Journal:  Crit Care Med       Date:  2001-07       Impact factor: 7.598

4.  Real-time quantitative broad-range PCR assay for detection of the 16S rRNA gene followed by sequencing for species identification.

Authors:  Franziska Zucol; Roland A Ammann; Christoph Berger; Christoph Aebi; Martin Altwegg; Felix K Niggli; David Nadal
Journal:  J Clin Microbiol       Date:  2006-08       Impact factor: 5.948

5.  Rapid identification of biothreat and other clinically relevant bacterial species by use of universal PCR coupled with high-resolution melting analysis.

Authors:  Samuel Yang; Padmini Ramachandran; Richard Rothman; Yu-Hsiang Hsieh; Andrew Hardick; Helen Won; Aleksandar Kecojevic; Joany Jackman; Charlotte Gaydos
Journal:  J Clin Microbiol       Date:  2009-05-20       Impact factor: 5.948

6.  Accurate and rapid identification of bacterial species from positive blood cultures with a DNA-based microarray platform: an observational study.

Authors:  Päivi Tissari; Alimuddin Zumla; Eveliina Tarkka; Sointu Mero; Laura Savolainen; Martti Vaara; Anne Aittakorpi; Sanna Laakso; Merja Lindfors; Heli Piiparinen; Minna Mäki; Caroline Carder; Jim Huggett; Vanya Gant
Journal:  Lancet       Date:  2009-12-10       Impact factor: 79.321

Review 7.  Septic shock.

Authors:  Djillali Annane; Eric Bellissant; Jean-Marc Cavaillon
Journal:  Lancet       Date:  2005 Jan 1-7       Impact factor: 79.321

Review 8.  Rapid methods for diagnosis of bloodstream infections.

Authors:  Mariam Klouche; Uwe Schröder
Journal:  Clin Chem Lab Med       Date:  2008       Impact factor: 3.694

Review 9.  Pathogens associated with sepsis in newborns and young infants in developing countries.

Authors:  Anita K M Zaidi; Durrane Thaver; Syed Asad Ali; Tauseef Ahmed Khan
Journal:  Pediatr Infect Dis J       Date:  2009-01       Impact factor: 2.129

10.  Direct identification of bacteria in positive blood culture bottles by matrix-assisted laser desorption ionisation time-of-flight mass spectrometry.

Authors:  Bernard La Scola; Didier Raoult
Journal:  PLoS One       Date:  2009-11-25       Impact factor: 3.240
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  22 in total

1.  Comparative analysis of two broad-range PCR assays for pathogen detection in positive-blood-culture bottles: PCR-high-resolution melting analysis versus PCR-mass spectrometry.

Authors:  Kevin Jeng; Charlotte A Gaydos; Lawrence B Blyn; Samuel Yang; Helen Won; Heather Matthews; Donna Toleno; Yu-Hsiang Hsieh; Karen C Carroll; Justin Hardick; Billy Masek; Alexander Kecojevic; Rangarajan Sampath; Stephen Peterson; Richard E Rothman
Journal:  J Clin Microbiol       Date:  2012-08-01       Impact factor: 5.948

2.  Application of a 16S rRNA PCR-high-resolution melt analysis assay for rapid detection of Salmonella Bacteremia.

Authors:  Kevin Jeng; Samuel Yang; Helen Won; Charlotte A Gaydos; Yu-Hsiang Hsieh; Alex Kecojevic; Karen C Carroll; Justin Hardick; Richard E Rothman
Journal:  J Clin Microbiol       Date:  2012-01-11       Impact factor: 5.948

3.  The use of procalcitonin as a marker of sepsis in children.

Authors:  Bin Mathew; Dinesh Roy D; T Vijaya Kumar
Journal:  J Clin Diagn Res       Date:  2013-02-01

Review 4.  A 'culture' shift: Application of molecular techniques for diagnosing polymicrobial infections.

Authors:  Yi Zhang; Anne Hu; Nadya Andini; Samuel Yang
Journal:  Biotechnol Adv       Date:  2019-02-20       Impact factor: 14.227

5.  Emerging Microtechnologies and Automated Systems for Rapid Bacterial Identification and Antibiotic Susceptibility Testing.

Authors:  Yiyan Li; Xing Yang; Weian Zhao
Journal:  SLAS Technol       Date:  2017-08-29       Impact factor: 3.047

6.  Sensitive detection and serovar differentiation of typhoidal and nontyphoidal Salmonella enterica species using 16S rRNA Gene PCR coupled with high-resolution melt analysis.

Authors:  Billie J Masek; Justin Hardick; Helen Won; Samuel Yang; Yu-Hsiang Hsieh; Richard E Rothman; Charlotte A Gaydos
Journal:  J Mol Diagn       Date:  2013-12-21       Impact factor: 5.568

7.  Targeted Treatment for Bacterial Infections: Prospects for Pathogen-Specific Antibiotics Coupled with Rapid Diagnostics.

Authors:  Tucker Maxson; Douglas A Mitchell
Journal:  Tetrahedron       Date:  2015-10-09       Impact factor: 2.457

8.  A Multiplex Electrochemical Biosensor for Bloodstream Infection Diagnosis.

Authors:  Jian Gao; Lindsie Jeffries; Kathleen E Mach; David W Craft; Neal J Thomas; Vincent Gau; Joseph C Liao; Pak Kin Wong
Journal:  SLAS Technol       Date:  2016-05-25       Impact factor: 3.047

9.  Identification of bacterial pathogens in ascitic fluids from patients with suspected spontaneous bacterial peritonitis by use of broad-range PCR (16S PCR) coupled with high-resolution melt analysis.

Authors:  Justin Hardick; Helen Won; Kevin Jeng; Yu-Hsiang Hsieh; Charlotte A Gaydos; Richard E Rothman; Samuel Yang
Journal:  J Clin Microbiol       Date:  2012-05-09       Impact factor: 5.948

10.  High-resolution melting molecular signatures for rapid identification of human papillomavirus genotypes.

Authors:  Ta-Hsien Lee; Tzong-Shoon Wu; Ching-Ping Tseng; Jiantai Timothy Qiu
Journal:  PLoS One       Date:  2012-08-20       Impact factor: 3.240
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