Literature DB >> 24478414

Multiplex high-resolution melting analysis as a diagnostic tool for detection of plasmid-mediated AmpC β-lactamase genes.

Chelsie N Geyer1, Nancy D Hanson.   

Abstract

High-resolution melting (HRM) analysis can be a diagnostic tool to evaluate the presence of resistance genes with the added bonus of discriminating sequence modifications. A real-time, multiplex PCR assay using HRM was designed for the detection of plasmid-mediated ampC genes. The specificity and sensitivity of this assay were 96% and 100%, respectively.

Mesh：

Substances：

Year:  2014        PMID： 24478414      PMCID： PMC3993522          DOI： 10.1128/JCM.00214-14

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


  15 in total

1.  Detection of plasmid-mediated AmpC beta-lactamase genes in clinical isolates by using multiplex PCR.

Authors:  F Javier Pérez-Pérez; Nancy D Hanson
Journal:  J Clin Microbiol       Date:  2002-06       Impact factor: 5.948

Review 2.  AmpC beta-lactamases: what do we need to know for the future?

Authors:  Nancy D Hanson
Journal:  J Antimicrob Chemother       Date:  2003-05-29       Impact factor: 5.790

3.  Rapid identification of biothreat and other clinically relevant bacterial species by use of universal PCR coupled with high-resolution melting analysis.

Authors:  Samuel Yang; Padmini Ramachandran; Richard Rothman; Yu-Hsiang Hsieh; Andrew Hardick; Helen Won; Aleksandar Kecojevic; Joany Jackman; Charlotte Gaydos
Journal:  J Clin Microbiol       Date:  2009-05-20       Impact factor: 5.948

4.  Development of a TaqMan multiplex PCR assay for detection of plasmid-mediated ampC β-lactamase genes.

Authors:  Chelsie N Geyer; Mark D Reisbig; Nancy D Hanson
Journal:  J Clin Microbiol       Date:  2012-08-15       Impact factor: 5.948

5.  ScreenClust: Advanced statistical software for supervised and unsupervised high resolution melting (HRM) analysis.

Authors:  Valin Reja; Alister Kwok; Glenn Stone; Linsong Yang; Andreas Missel; Christoph Menzel; Brant Bassam
Journal:  Methods       Date:  2010-02-08       Impact factor: 3.608

6.  Using newly developed multiplex polymerase chain reaction and melting curve analysis for detection and discrimination of β-lactamases in Escherichia coli isolates from intensive care patients.

Authors:  Magdaléna Chromá; Kristýna Hricová; Milan Kolář; Pavel Sauer; Dagmar Koukalová
Journal:  Diagn Microbiol Infect Dis       Date:  2011-11       Impact factor: 2.803

7.  Rapid detection and statistical differentiation of KPC gene variants in Gram-negative pathogens by use of high-resolution melting and ScreenClust analyses.

Authors:  Amanda L Roth; Nancy D Hanson
Journal:  J Clin Microbiol       Date:  2012-10-17       Impact factor: 5.948

8.  The cost-effectiveness of ESBL detection: towards molecular detection methods?

Authors:  B B Wintermans; E A Reuland; R G F Wintermans; A M C Bergmans; J A J W Kluytmans
Journal:  Clin Microbiol Infect       Date:  2012-09-12       Impact factor: 8.067

9.  Point mutations in the inc antisense RNA gene are associated with increased plasmid copy number, expression of blaCMY-2 and resistance to piperacillin/tazobactam in Escherichia coli.

Authors:  Philip M Kurpiel; Nancy D Hanson
Journal:  J Antimicrob Chemother       Date:  2011-11-24       Impact factor: 5.790

10.  Phenotypic and biochemical comparison of the carbapenem-hydrolyzing activities of five plasmid-borne AmpC β-lactamases.

Authors:  Hedi Mammeri; Hélène Guillon; François Eb; Patrice Nordmann
Journal:  Antimicrob Agents Chemother       Date:  2010-08-23       Impact factor: 5.191
View more
  6 in total

1.  Multiplex PCR scheme for variant plasmid mediated class C β-lactamase typing.

Authors:  Le Van Chuong; Virapong Prachayasittikul; Chartchalerm Isarankura Na Ayudhya; Ratana Lawung
Journal:  J Clin Lab Anal       Date:  2017-07-18       Impact factor: 2.352

2.  Coordination of las regulated virulence factors with Multidrug-Resistant and extensively drug-resistant in superbug strains of P. aeruginosa.

Authors:  Sanaz Dehbashi; Mohammad Reza Pourmand; Mohammad Yousef Alikhani; Sara Soleimani Asl; Mohammad Reza Arabestani
Journal:  Mol Biol Rep       Date:  2020-05-30       Impact factor: 2.742

3.  Identification and quantification of virulence factors of enterotoxigenic Escherichia coli by high-resolution melting curve quantitative PCR.

Authors:  Weilan Wang; Ruurd T Zijlstra; Michael G Gänzle
Journal:  BMC Microbiol       Date:  2017-05-15       Impact factor: 3.605

4.  Association between Beta-lactam Antibiotic Resistance and Virulence Factors in AmpC Producing Clinical Strains of P. aeruginosa.

Authors:  Sanaz Dehbashi; Hamed Tahmasebi; Mohammad Reza Arabestani
Journal:  Osong Public Health Res Perspect       Date:  2018-12

5.  A High Resolution DNA Melting Curve Analysis for the Rapid and Efficient Molecular Diagnostics of Extended Spectrum β-Lactamase Determinants from Foodborne Escherichia coli.

Authors:  Patrick Murigu Kamau Njage; Elna Buys
Journal:  Microorganisms       Date:  2020-01-09

6.  Co-Occurrence of Plasmid-Mediated AmpC β-Lactamase Activity Among Klebsiella pneumoniae and Escherichia Coli.

Authors:  Abdulaziz Zorgani; Hiyam Daw; Najib Sufya; Abdullah Bashein; Omar Elahmer; Chedly Chouchani
Journal:  Open Microbiol J       Date:  2017-09-26
  6 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.