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Literature DB >> 19374550

A primer for morpholino use in zebrafish.

Brent R Bill¹, Andrew M Petzold, Karl J Clark, Lisa A Schimmenti, Stephen C Ekker.

Abstract

Morpholino oligonucleotides are the most common anti-sense "knockdown" technique used in zebrafish (Danio rerio). This review discusses common practices for the design, preparation, and deployment of morpholinos in this vertebrate model system. Off-targeting effects of morpholinos are discussed as well as method to minimize this potentially confounding variable via co-injection of a tP53-targeting morpholino. Finally, new uses of morpholinos are summarized and contextualized with respect to the complementary, DNA-based knockout technologies recently developed for zebrafish.

Entities: Chemical Disease Gene Species

Mesh：

Substances：
Oligonucleotides

Year: 2009 PMID： 19374550 PMCID： PMC2776066 DOI： 10.1089/zeb.2008.0555

Source DB: PubMed Journal: Zebrafish ISSN： 1545-8547 Impact factor: 1.985

48 in total

1. Achieving targeted and quantifiable alteration of mRNA splicing with Morpholino oligos.

Authors: Paul A Morcos
Journal: Biochem Biophys Res Commun Date: 2007-05-07 Impact factor: 3.575

2. Morpholino and phosphorothioate antisense oligomers compared in cell-free and in-cell systems.

Authors: J Summerton; D Stein; S B Huang; P Matthews; D Weller; M Partridge
Journal: Antisense Nucleic Acid Drug Dev Date: 1997-04

3. Inhibition of zebrafish fin regeneration using in vivo electroporation of morpholinos against fgfr1 and msxb.

Authors: Ryan Thummel; Shan Bai; Michael P Sarras; Peizhen Song; Jeffrey McDermott; Jeffrey Brewer; Martin Perry; Xiaoming Zhang; David R Hyde; Alan R Godwin
Journal: Dev Dyn Date: 2006-02 Impact factor: 3.780

4. A simple method for delivering morpholino antisense oligos into the cytoplasm of cells.

Authors: M Partridge; A Vincent; P Matthews; J Puma; D Stein; J Summerton
Journal: Antisense Nucleic Acid Drug Dev Date: 1996

5. heart of glass regulates the concentric growth of the heart in zebrafish.

Authors: John D Mably; Manzoor Ali P K Mohideen; C Geoffrey Burns; Jau-Nian Chen; Mark C Fishman
Journal: Curr Biol Date: 2003-12-16 Impact factor: 10.834

6. The T box transcription factor no tail in ciliated cells controls zebrafish left-right asymmetry.

Authors: Jeffrey D Amack; H Joseph Yost
Journal: Curr Biol Date: 2004-04-20 Impact factor: 10.834

Review 7. Functional genomics tools for the analysis of zebrafish pigment.

Authors: Michael A Pickart; Sridhar Sivasubbu; Aubrey L Nielsen; Sabitha Shriram; Richard A King; Stephen C Ekker
Journal: Pigment Cell Res Date: 2004-10

8. Annexin 2-caveolin 1 complex is a target of ezetimibe and regulates intestinal cholesterol transport.

Authors: Eric J Smart; Robert A De Rose; Steven A Farber
Journal: Proc Natl Acad Sci U S A Date: 2004-02-25 Impact factor: 11.205

9. dino and mercedes, two genes regulating dorsal development in the zebrafish embryo.

Authors: M Hammerschmidt; F Pelegri; M C Mullins; D A Kane; F J van Eeden; M Granato; M Brand; M Furutani-Seiki; P Haffter; C P Heisenberg; Y J Jiang; R N Kelsh; J Odenthal; R M Warga; C Nüsslein-Volhard
Journal: Development Date: 1996-12 Impact factor: 6.868

10. Genome-wide reverse genetics framework to identify novel functions of the vertebrate secretome.

Authors: Michael A Pickart; Eric W Klee; Aubrey L Nielsen; Sridhar Sivasubbu; Eric M Mendenhall; Brent R Bill; Eleanor Chen; Craig E Eckfeldt; Michelle Knowlton; Mara E Robu; Jon D Larson; Yun Deng; Lisa A Schimmenti; Lynda B M Ellis; Catherine M Verfaillie; Matthias Hammerschmidt; Steven A Farber; Stephen C Ekker
Journal: PLoS One Date: 2006-12-20 Impact factor: 3.240

167 in total

1. Gene knockdown by morpholino-modified oligonucleotides in the zebrafish (Danio rerio) model: applications for developmental toxicology.

Authors: Alicia R Timme-Laragy; Sibel I Karchner; Mark E Hahn
Journal: Methods Mol Biol Date: 2012

2. Homozygous splice mutation in CWF19L1 in a Turkish family with recessive ataxia syndrome.

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Journal: Neurology Date: 2014-10-31 Impact factor: 9.910

Review 3. Modeling human neurodegenerative diseases in transgenic systems.

Authors: Miguel A Gama Sosa; Rita De Gasperi; Gregory A Elder
Journal: Hum Genet Date: 2011-12-14 Impact factor: 4.132

4. Morpholino injection in Xenopus.

Authors: Panna Tandon; Chris Showell; Kathleen Christine; Frank L Conlon
Journal: Methods Mol Biol Date: 2012

5. Cyclic caged morpholinos: conformationally gated probes of embryonic gene function.

Authors: Sayumi Yamazoe; Ilya A Shestopalov; Elayne Provost; Steven D Leach; James K Chen
Journal: Angew Chem Int Ed Engl Date: 2012-06-11 Impact factor: 15.336

Review 6. Reverse genetics in eukaryotes.

Authors: Serge Hardy; Vincent Legagneux; Yann Audic; Luc Paillard
Journal: Biol Cell Date: 2010-10 Impact factor: 4.458