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Literature DB >> 19349978

An ultramarine fluorescent protein with increased photostability and pH insensitivity.

Wataru Tomosugi¹, Tomoki Matsuda, Tomomi Tani, Tomomi Nemoto, Ippei Kotera, Kenta Saito, Kazuki Horikawa, Takeharu Nagai.

Abstract

We report a pH-insensitive and photostable ultramarine fluorescent protein, Sirius, with an emission peak at 424 nm, the shortest emission wavelength among fluorescent proteins reported to date. The pH-insensitivity of Sirius allowed prolonged visualization of biological events in an acidic environment. Two fluorescence resonance energy transfer (FRET) pairs, Sirius-mseCFP and Sapphire-DsRed, allowed dual-FRET imaging with single-wavelength excitation, enabling detection of Ca(2+) concentration and caspase-3 activation in the same apoptotic cells.

Entities: Chemical Gene

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Year: 2009 PMID： 19349978 DOI： 10.1038/nmeth.1317

Source DB: PubMed Journal: Nat Methods ISSN： 1548-7091 Impact factor: 28.547

10 in total

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An ultramarine fluorescent protein with increased photostability and pH insensitivity.

1. Red fluorescent protein from Discosoma as a fusion tag and a partner for fluorescence resonance energy transfer.

Review 2. Spectral imaging and its applications in live cell microscopy.

3. Direct measurement of protein dynamics inside cells using a rationally designed photoconvertible protein.

4. A variant of yellow fluorescent protein with fast and efficient maturation for cell-biological applications.

5. Fluorescent protein FRET pairs for ratiometric imaging of dual biosensors.

6. A Ca2+ signal is found upstream of cytochrome c release during apoptosis in HeLa cells.

Review 7. Understanding, improving and using green fluorescent proteins.

8. Detection of programmed cell death using fluorescence energy transfer.

9. Exploration of new chromophore structures leads to the identification of improved blue fluorescent proteins.

10. Spatio-temporal activation of caspase revealed by indicator that is insensitive to environmental effects.

1. Red fluorescent proteins (RFPs) and RFP-based biosensors for neuronal imaging applications.

2. Development of UV-excitable red and near-infrared fluorescent labels and their application for simultaneous multicolor bioimaging by single-wavelength excitation.

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