Literature DB >> 25894946

A fast- and positively photoswitchable fluorescent protein for ultralow-laser-power RESOLFT nanoscopy.

Dhermendra K Tiwari1, Yoshiyuki Arai1, Masahito Yamanaka2, Tomoki Matsuda1, Masakazu Agetsuma1, Masahiro Nakano1, Katsumasa Fujita2, Takeharu Nagai1.   

Abstract

Fluorescence nanoscopy has revolutionized our ability to visualize biological structures not resolvable by conventional microscopy. However, photodamage induced by intense light exposure has limited its use in live specimens. Here we describe Kohinoor, a fast-switching, positively photoswitchable fluorescent protein, and show that it has high photostability over many switching repeats. With Kohinoor, we achieved super-resolution imaging of live HeLa cells using biocompatible, ultralow laser intensity (0.004 J/cm(2)) in reversible saturable optical fluorescence transition (RESOLFT) nanoscopy.

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Year:  2015        PMID： 25894946     DOI： 10.1038/nmeth.3362

Source DB:  PubMed          Journal:  Nat Methods        ISSN： 1548-7091            Impact factor:   28.547


  25 in total

1.  Molecular basis of the light-driven switching of the photochromic fluorescent protein Padron.

Authors:  Tanja Brakemann; Gert Weber; Martin Andresen; Gerrit Groenhof; Andre C Stiel; Simon Trowitzsch; Christian Eggeling; Helmut Grubmüller; Stefan W Hell; Markus C Wahl; Stefan Jakobs
Journal:  J Biol Chem       Date:  2010-03-16       Impact factor: 5.157

2.  1.8 A bright-state structure of the reversibly switchable fluorescent protein Dronpa guides the generation of fast switching variants.

Authors:  Andre C Stiel; Simon Trowitzsch; Gert Weber; Martin Andresen; Christian Eggeling; Stefan W Hell; Stefan Jakobs; Markus C Wahl
Journal:  Biochem J       Date:  2007-02-15       Impact factor: 3.857

3.  Wide-field subdiffraction RESOLFT microscopy using fluorescent protein photoswitching.

Authors:  Miriam A Schwentker; Hannes Bock; Michael Hofmann; Stefan Jakobs; Jörg Bewersdorf; Christian Eggeling; Stefan W Hell
Journal:  Microsc Res Tech       Date:  2007-03       Impact factor: 2.769

4.  Direct measurement of protein dynamics inside cells using a rationally designed photoconvertible protein.

Authors:  Tomoki Matsuda; Atsushi Miyawaki; Takeharu Nagai
Journal:  Nat Methods       Date:  2008-03-16       Impact factor: 28.547

Review 5.  Smart fluorescent proteins: innovation for barrier-free superresolution imaging in living cells.

Authors:  Dhermendra K Tiwari; Takeharu Nagai
Journal:  Dev Growth Differ       Date:  2013-05-02       Impact factor: 2.053

6.  Molecular evolution by staggered extension process (StEP) in vitro recombination.

Authors:  H Zhao; L Giver; Z Shao; J A Affholter; F H Arnold
Journal:  Nat Biotechnol       Date:  1998-03       Impact factor: 54.908

7.  Spatially isotropic four-dimensional imaging with dual-view plane illumination microscopy.

Authors:  Yicong Wu; Peter Wawrzusin; Justin Senseney; Robert S Fischer; Ryan Christensen; Anthony Santella; Andrew G York; Peter W Winter; Clare M Waterman; Zhirong Bao; Daniel A Colón-Ramos; Matthew McAuliffe; Hari Shroff
Journal:  Nat Biotechnol       Date:  2013-10-13       Impact factor: 54.908

8.  Lattice light-sheet microscopy: imaging molecules to embryos at high spatiotemporal resolution.

Authors:  Bi-Chang Chen; Wesley R Legant; Kai Wang; Lin Shao; Daniel E Milkie; Michael W Davidson; Chris Janetopoulos; Xufeng S Wu; John A Hammer; Zhe Liu; Brian P English; Yuko Mimori-Kiyosue; Daniel P Romero; Alex T Ritter; Jennifer Lippincott-Schwartz; Lillian Fritz-Laylin; R Dyche Mullins; Diana M Mitchell; Joshua N Bembenek; Anne-Cecile Reymann; Ralph Böhme; Stephan W Grill; Jennifer T Wang; Geraldine Seydoux; U Serdar Tulu; Daniel P Kiehart; Eric Betzig
Journal:  Science       Date:  2014-10-23       Impact factor: 47.728

9.  rsEGFP2 enables fast RESOLFT nanoscopy of living cells.

Authors:  Tim Grotjohann; Ilaria Testa; Matthias Reuss; Tanja Brakemann; Christian Eggeling; Stefan W Hell; Stefan Jakobs
Journal:  Elife       Date:  2012-12-31       Impact factor: 8.140

10.  A bright monomeric green fluorescent protein derived from Branchiostoma lanceolatum.

Authors:  Nathan C Shaner; Gerard G Lambert; Andrew Chammas; Yuhui Ni; Paula J Cranfill; Michelle A Baird; Brittney R Sell; John R Allen; Richard N Day; Maria Israelsson; Michael W Davidson; Jiwu Wang
Journal:  Nat Methods       Date:  2013-03-24       Impact factor: 28.547
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  24 in total

Review 1.  Photostable and photoswitching fluorescent dyes for super-resolution imaging.

Authors:  Masafumi Minoshima; Kazuya Kikuchi
Journal:  J Biol Inorg Chem       Date:  2017-01-12       Impact factor: 3.358

2.  Out-of-Phase Imaging after Optical Modulation (OPIOM) for Multiplexed Fluorescence Imaging Under Adverse Optical Conditions.

Authors:  Raja Chouket; Ruikang Zhang; Agnès Pellissier-Tanon; Annie Lemarchand; Agathe Espagne; Thomas Le Saux; Ludovic Jullien
Journal:  Methods Mol Biol       Date:  2021

Review 3.  Photoswitchable Fluorescent Proteins: Mechanisms on Ultrafast Timescales.

Authors:  Longteng Tang; Chong Fang
Journal:  Int J Mol Sci       Date:  2022-06-09       Impact factor: 6.208

Review 4.  Fluorescent probes for nanoscopy: four categories and multiple possibilities.

Authors:  Ming Ni; Shuangmu Zhuo; Peter T C So; Hanry Yu
Journal:  J Biophotonics       Date:  2016-05-25       Impact factor: 3.207

Review 5.  Technological advances in super-resolution microscopy to study cellular processes.

Authors:  Charles Bond; Adriana N Santiago-Ruiz; Qing Tang; Melike Lakadamyali
Journal:  Mol Cell       Date:  2022-01-20       Impact factor: 17.970

6.  RESOLFT Nanoscopy of Fixed Cells Using a Z-Domain Based Fusion Protein for Labelling.

Authors:  Peter Ilgen; Tim Grotjohann; Daniel C Jans; Markus Kilisch; Stefan W Hell; Stefan Jakobs
Journal:  PLoS One       Date:  2015-09-16       Impact factor: 3.240

7.  Ultrasmall all-optical plasmonic switch and its application to superresolution imaging.

Authors:  Hsueh-Yu Wu; Yen-Ta Huang; Po-Ting Shen; Hsuan Lee; Ryosuke Oketani; Yasuo Yonemaru; Masahito Yamanaka; Satoru Shoji; Kung-Hsuan Lin; Chih-Wei Chang; Satoshi Kawata; Katsumasa Fujita; Shi-Wei Chu
Journal:  Sci Rep       Date:  2016-04-11       Impact factor: 4.379

8.  In vivo super-resolution RESOLFT microscopy of Drosophila melanogaster.

Authors:  Sebastian Schnorrenberg; Tim Grotjohann; Gerd Vorbrüggen; Alf Herzig; Stefan W Hell; Stefan Jakobs
Journal:  Elife       Date:  2016-06-29       Impact factor: 8.140

9.  Two-photon-like microscopy with orders-of-magnitude lower illumination intensity via two-step fluorescence.

Authors:  Maria Ingaramo; Andrew G York; Eric J Andrade; Kristin Rainey; George H Patterson
Journal:  Nat Commun       Date:  2015-09-03       Impact factor: 14.919

10.  Rational design of ultrastable and reversibly photoswitchable fluorescent proteins for super-resolution imaging of the bacterial periplasm.

Authors:  Mariam El Khatib; Alexandre Martins; Dominique Bourgeois; Jacques-Philippe Colletier; Virgile Adam
Journal:  Sci Rep       Date:  2016-01-06       Impact factor: 4.379
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