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Literature DB >> 9518501

Detection of programmed cell death using fluorescence energy transfer.

X Xu¹, A L Gerard, B C Huang, D C Anderson, D G Payan, Y Luo.

Abstract

Fluorescence energy transfer (FRET) can be generated when green fluorescent protein (GFP) and blue fluorescent protein (BFP) are covalently linked together by a short peptide. Cleavage of this linkage by protease completely eliminates FRET effect. Caspase-3 (CPP32) is an important cellular protease activated during programmed cell death. An 18 amino acid peptide containing CPP32 recognition sequence, DEVD, was used to link GFP and BFP together. CPP32 activation can be monitored by FRET assay during the apoptosis process.

Entities: Chemical Gene

Mesh：

Substances：

Year: 1998 PMID： 9518501 PMCID： PMC147488 DOI： 10.1093/nar/26.8.2034

Source DB: PubMed Journal: Nucleic Acids Res ISSN： 0305-1048 Impact factor: 16.971

8 in total

1. Fluorescent indicators for Ca2+ based on green fluorescent proteins and calmodulin.

Authors: A Miyawaki; J Llopis; R Heim; J M McCaffery; J A Adams; M Ikura; R Y Tsien
Journal: Nature Date: 1997-08-28 Impact factor: 49.962

2. RIP: a novel protein containing a death domain that interacts with Fas/APO-1 (CD95) in yeast and causes cell death.

Authors: B Z Stanger; P Leder; T H Lee; E Kim; B Seed
Journal: Cell Date: 1995-05-19 Impact factor: 41.582

3. Engineering green fluorescent protein for improved brightness, longer wavelengths and fluorescence resonance energy transfer.

Authors: R Heim; R Y Tsien
Journal: Curr Biol Date: 1996-02-01 Impact factor: 10.834

Review 4. Applications of the green fluorescent protein in cell biology and biotechnology.

Authors: T Misteli; D L Spector
Journal: Nat Biotechnol Date: 1997-10 Impact factor: 54.908

Review 5. Apoptosis by death factor.

Authors: S Nagata
Journal: Cell Date: 1997-02-07 Impact factor: 41.582

Review 6. Protease activation during apoptosis: death by a thousand cuts?

Authors: S J Martin; D R Green
Journal: Cell Date: 1995-08-11 Impact factor: 41.582

7. Activation of actin-cleavable interleukin 1beta-converting enzyme (ICE) family protease CPP-32 during chemotherapeutic agent-induced apoptosis in ovarian carcinoma cells.

Authors: Z Chen; M Naito; T Mashima; T Tsuruo
Journal: Cancer Res Date: 1996-11-15 Impact factor: 12.701

8. Sequential activation of ICE-like and CPP32-like proteases during Fas-mediated apoptosis.

Authors: M Enari; R V Talanian; W W Wong; S Nagata
Journal: Nature Date: 1996-04-25 Impact factor: 49.962

8 in total

39 in total

1. Marking synaptic activity in dendritic spines with a calpain substrate exhibiting fluorescence resonance energy transfer.

Authors: P W Vanderklish; L A Krushel; B H Holst; J A Gally; K L Crossin; G M Edelman
Journal: Proc Natl Acad Sci U S A Date: 2000-02-29 Impact factor: 11.205

2. Measurement of proteases using chemiluminescence-resonance-energy-transfer chimaeras between green fluorescent protein and aequorin.

Authors: J P Waud; A Bermúdez Fajardo; T Sudhaharan; A R Trimby; J Jeffery; A Jones; A K Campbell
Journal: Biochem J Date: 2001-08-01 Impact factor: 3.857

3. In situ characterization of genetically targeted (green fluorescent) single cells and their microenvironment in an adoptive host.

Authors: F Leithäuser; Z Trobonjaca; J Reimann; P Möller
Journal: Am J Pathol Date: 2001-06 Impact factor: 4.307

4. Confirmation by FRET in individual living cells of the absence of significant amyloid beta -mediated caspase 8 activation.

Authors: Reiko Onuki; Akira Nagasaki; Hiroaki Kawasaki; Tadashi Baba; Taro Q P Uyeda; Kazunari Taira
Journal: Proc Natl Acad Sci U S A Date: 2002-10-30 Impact factor: 11.205

Detection of programmed cell death using fluorescence energy transfer.

1. Fluorescent indicators for Ca2+ based on green fluorescent proteins and calmodulin.

2. RIP: a novel protein containing a death domain that interacts with Fas/APO-1 (CD95) in yeast and causes cell death.

3. Engineering green fluorescent protein for improved brightness, longer wavelengths and fluorescence resonance energy transfer.

Review 4. Applications of the green fluorescent protein in cell biology and biotechnology.

Review 5. Apoptosis by death factor.

Review 6. Protease activation during apoptosis: death by a thousand cuts?

7. Activation of actin-cleavable interleukin 1beta-converting enzyme (ICE) family protease CPP-32 during chemotherapeutic agent-induced apoptosis in ovarian carcinoma cells.

8. Sequential activation of ICE-like and CPP32-like proteases during Fas-mediated apoptosis.

1. Marking synaptic activity in dendritic spines with a calpain substrate exhibiting fluorescence resonance energy transfer.

2. Measurement of proteases using chemiluminescence-resonance-energy-transfer chimaeras between green fluorescent protein and aequorin.

3. In situ characterization of genetically targeted (green fluorescent) single cells and their microenvironment in an adoptive host.

4. Confirmation by FRET in individual living cells of the absence of significant amyloid beta -mediated caspase 8 activation.

5. Far-red fluorescent tag for protein labelling.

6. A fluorogenic red fluorescent protein heterodimer.

Review 7. Spatial organization of intracellular communication: insights from imaging.

8. Exercise-induced immune cell apoptosis: image-based model for morphological assessment.

9. An experimental study of GFP-based FRET, with application to intrinsically unstructured proteins.

10. An ultramarine fluorescent protein with increased photostability and pH insensitivity.