Literature DB >> 19243101

Fast two-dimensional NMR spectroscopy of high molecular weight protein assemblies.

Carlos Amero1, Paul Schanda, M Asunción Durá, Isabel Ayala, Dominique Marion, Bruno Franzetti, Bernhard Brutscher, Jérôme Boisbouvier.   

Abstract

An optimized NMR experiment that combines the advantages of methyl-TROSY and SOFAST-HMQC has been developed. It allows the recording of high quality methyl (1)H-(13)C correlation spectra of protein assemblies of several hundreds of kDa in a few seconds. The SOFAST-methyl-TROSY-based experiment offers completely new opportunities for the study of structural and dynamic changes occurring in molecular nanomachines while they perform their biological function in vitro.

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Year:  2009        PMID: 19243101     DOI: 10.1021/ja809880p

Source DB:  PubMed          Journal:  J Am Chem Soc        ISSN: 0002-7863            Impact factor:   15.419


  37 in total

1.  High resolution methyl selective ¹³C-NMR of proteins in solution and solid state.

Authors:  Garima Jaipuria; Nitin Prakash Lobo; Divya Shet; Hanudatta S Atreya
Journal:  J Biomol NMR       Date:  2012-07-11       Impact factor: 2.835

2.  Optimal methyl labeling for studies of supra-molecular systems.

Authors:  Tomasz L Religa; Lewis E Kay
Journal:  J Biomol NMR       Date:  2010-04-27       Impact factor: 2.835

3.  Advances in Nuclear Magnetic Resonance for Drug Discovery.

Authors:  Robert Powers
Journal:  Expert Opin Drug Discov       Date:  2009-10-01       Impact factor: 6.098

4.  High-resolution NMR field-cycling device for full-range relaxation and structural studies of biopolymers on a shared commercial instrument.

Authors:  Alfred G Redfield
Journal:  J Biomol NMR       Date:  2011-12-27       Impact factor: 2.835

Review 5.  Methyl groups as probes of supra-molecular structure, dynamics and function.

Authors:  Amy M Ruschak; Lewis E Kay
Journal:  J Biomol NMR       Date:  2009-09-27       Impact factor: 2.835

6.  A systematic mutagenesis-driven strategy for site-resolved NMR studies of supramolecular assemblies.

Authors:  Carlos Amero; M Asunción Durá; Marjolaine Noirclerc-Savoye; Arnaud Perollier; Benoit Gallet; Michael J Plevin; Thierry Vernet; Bruno Franzetti; Jérôme Boisbouvier
Journal:  J Biomol NMR       Date:  2011-05-29       Impact factor: 2.835

7.  Allosteric enhancement of MAP kinase p38α's activity and substrate selectivity by docking interactions.

Authors:  Yuji Tokunaga; Koh Takeuchi; Hideo Takahashi; Ichio Shimada
Journal:  Nat Struct Mol Biol       Date:  2014-07-20       Impact factor: 15.369

8.  Solution Structure of the Carboxy-Terminal Tandem Repeat Domain of Eukaryotic Elongation Factor 2 Kinase and Its Role in Substrate Recognition.

Authors:  Andrea Piserchio; Nathan Will; David H Giles; Fatlum Hajredini; Kevin N Dalby; Ranajeet Ghose
Journal:  J Mol Biol       Date:  2019-05-18       Impact factor: 5.469

9.  15N and 13C- SOFAST-HMQC editing enhances 3D-NOESY sensitivity in highly deuterated, selectively [1H,13C]-labeled proteins.

Authors:  Paolo Rossi; Youlin Xia; Nandish Khanra; Gianluigi Veglia; Charalampos G Kalodimos
Journal:  J Biomol NMR       Date:  2016-11-23       Impact factor: 2.835

Review 10.  Perspectives in magnetic resonance: NMR in the post-FFT era.

Authors:  Sven G Hyberts; Haribabu Arthanari; Scott A Robson; Gerhard Wagner
Journal:  J Magn Reson       Date:  2014-04       Impact factor: 2.229

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