Warning: Undefined array key "mm" in /www/wwwroot/www.ai-bt.com/si.php on line 10 Deprecated: trim(): Passing null to parameter #1 ($string) of type string is deprecated in /www/wwwroot/www.ai-bt.com/si.php on line 10 UGA is translated as cysteine in pheromone 3 of Euplotes octocarinatus.

Literature DB >> 1902568

UGA is translated as cysteine in pheromone 3 of Euplotes octocarinatus.

F Meyer¹, H J Schmidt, E Plümper, A Hasilik, G Mersmann, H E Meyer, A Engström, K Heckmann.

Abstract

Pheromone 3 mRNA of the ciliate Euplotes octocarinatus contains three in-frame UGA codons that are translated as cysteines. This was revealed from cDNA sequencing and from plasma desorption mass spectrometry of cleaved pheromone 3 in connection with pyridylethylation of the fragments. N-terminal sequence analysis of carboxymethylated protein confirmed this conclusion for the first of the three UGA codons. Besides UGA the common cysteine codons UGU and UGC are also used to encode cysteine. UAA functions as a termination codon. No UAG codon was found. In connection with results reported for other ciliates, this suggests that the role of the classic termination codons had not yet been established when the ciliates started to diverge from other eukaryotes.

Entities: Chemical Species

Mesh：

Substances：

Year: 1991 PMID： 1902568 PMCID： PMC51532 DOI： 10.1073/pnas.88.9.3758

Source DB: PubMed Journal: Proc Natl Acad Sci U S A ISSN： 0027-8424 Impact factor: 11.205

26 in total

1. Strong homology between the small subunit of ribulose-1,5-bisphosphate carboxylase/oxygenase of two species of Acetabularia and the occurrence of unusual codon usage.

Authors: S U Schneider; M B Leible; X P Yang
Journal: Mol Gen Genet Date: 1989-09

2. Gene for a novel tRNA species that accepts L-serine and cotranslationally inserts selenocysteine.

Authors: W Leinfelder; E Zehelein; M A Mandrand-Berthelot; A Böck
Journal: Nature Date: 1988-02-25 Impact factor: 49.962

3. Sequence of a cDNA coding for human glutathione peroxidase confirms TGA encodes active site selenocysteine.

Authors: G T Mullenbach; A Tabrizi; B D Irvine; G I Bell; R A Hallewell
Journal: Nucleic Acids Res Date: 1987-07-10 Impact factor: 16.971

4. The isolation of gamones 3 and 4 of Euplotes octocarinatus.

Authors: H Schulze Dieckhoff; M Freiburg; K Heckmann
Journal: Eur J Biochem Date: 1987-10-01

5. Determination of nucleotide sequence of cDNA coding rat glutathione peroxidase and diminished expression of the mRNA in selenium deficient rat liver.

Authors: S Yoshimura; S Takekoshi; K Watanabe; Y Fujii-Kuriyama
Journal: Biochem Biophys Res Commun Date: 1988-08-15 Impact factor: 3.575

6. Different pattern of codon recognition by mammalian mitochondrial tRNAs.

Authors: B G Barrell; S Anderson; A T Bankier; M H de Bruijn; E Chen; A R Coulson; J Drouin; I C Eperon; D P Nierlich; B A Roe; F Sanger; P H Schreier; A J Smith; R Staden; I G Young
Journal: Proc Natl Acad Sci U S A Date: 1980-06 Impact factor: 11.205

7. Cotranslational insertion of selenocysteine into formate dehydrogenase from Escherichia coli directed by a UGA codon.

Authors: F Zinoni; A Birkmann; W Leinfelder; A Böck
Journal: Proc Natl Acad Sci U S A Date: 1987-05 Impact factor: 11.205

8. Isolation and characterization of the actin gene from Tetrahymena thermophila.

Authors: C G Cupples; R E Pearlman
Journal: Proc Natl Acad Sci U S A Date: 1986-07 Impact factor: 11.205

9. The structure of the mouse glutathione peroxidase gene: the selenocysteine in the active site is encoded by the 'termination' codon, TGA.

Authors: I Chambers; J Frampton; P Goldfarb; N Affara; W McBain; P R Harrison
Journal: EMBO J Date: 1986-06 Impact factor: 11.598

10. Dramatic events in ciliate evolution: alteration of UAA and UAG termination codons to glutamine codons due to anticodon mutations in two Tetrahymena tRNAs.

Authors: N Hanyu; Y Kuchino; S Nishimura; H Beier
Journal: EMBO J Date: 1986-06 Impact factor: 11.598

44 in total

Review 1. Misreading of termination codons in eukaryotes by natural nonsense suppressor tRNAs.

Authors: H Beier; M Grimm
Journal: Nucleic Acids Res Date: 2001-12-01 Impact factor: 16.971

2. Identification, characterization, and complete amino acid sequence of the conjugation-inducing glycoprotein (blepharmone) in the ciliate Blepharisma japonicum.

Authors: M Sugiura; T Harumoto
Journal: Proc Natl Acad Sci U S A Date: 2001-11-27 Impact factor: 11.205

Review 3. How selenium has altered our understanding of the genetic code.

Authors: Dolph L Hatfield; Vadim N Gladyshev
Journal: Mol Cell Biol Date: 2002-06 Impact factor: 4.272

Review 4. Regulation of gene expression by stop codon recoding: selenocysteine.

Authors: Paul R Copeland
Journal: Gene Date: 2003-07-17 Impact factor: 3.688

5. TGA cysteine codons and intron sequences in conserved and nonconserved positions are found in macronuclear RNA polymerase genes of Euplotes octocarinatus.

Authors: J Kaufmann; V Florian; A Klein
Journal: Nucleic Acids Res Date: 1992-11-25 Impact factor: 16.971