| Literature DB >> 19002839 |
Keiko Momma1, Yuko Masuzawa, Naomi Nakai, Moeko Chujo, Akira Murakami, Noriyuki Kioka, Yasunori Kiyama, Toru Akita, Masaya Nagao.
Abstract
A methanol extract of Alsomitra macrocarpa leaves and branches induced a marked alteration of cell morphology in a human stellate cell line (LX-2). Similar morphologic alterations were observed in several other cell lines. Active compound was purified from the extract and determined to be cucurbitacin E (Cuc E). It has been known that Cuc E causes marked disruption of the actin cytoskeleton, supporting our observation, but how Cuc E altered the actin cytoskeleton has not been elucidated. By using the standard fluorescence assay using copolymerization and depolymerization of native and pyrene labelled actin, this study revealed that Cuc E interacted directly with actin consequently stabilizing the polymerized actin. When NIH-3T3 cells exogenously expressing YFP-labeled actin were treated with Cuc E, firstly the aggregation of globular actin and secondly the aggregation of actin including disrupted fibrous actin in the cells was observed.Entities:
Year: 2007 PMID： 19002839 PMCID： PMC2151965 DOI： 10.1007/s10616-007-9100-5
Source DB: PubMed Journal: Cytotechnology ISSN： 0920-9069 Impact factor: 2.058