Shahab Bohlooli1, Naser Jafari, Shahram Jahed. 1. Department of Pharmacology, School of Medicine, Ardabil University of Medical Sciences, University Street, Ardabil, 56197, Iran. shahab.bohlooli@arums.ac.ir
Abstract
PURPOSE: Ecballium elaterium (L.) A. Rich (Cucurbitaceae), also known as the "squirting cucumber," is a wild medicinal plant found abundantly in Moghan, Ardabil province, Iran. This study was undertaken to examine possible cytotoxic effect of freeze-dried aqueous extract of E. elaterium fruit on cell lines of gastric and esophageal origin namely called AGS (human gastric carcinoma) and KYSE30 (human esophageal squamous cell carcinoma). METHODS: The aqueous extract of the fruits of E. elaterium was prepared and freeze-dried. AGS and KYSE30 cancer cell lines were treated by the extract and incubated for 24, 48, and 72 h. Cytotoxicity was examined by MTT assay. Ethidium bromide/acridine orange (EB/AO) staining was used for apoptotic cell detection. A DAPI staining method was used to analyze cell cycle by flow cytometry. RESULTS: The IC(50) values were 2.5, 0.7, and 0.7 μg/ml for AGS cell line after 24, 48, and 72 h, respectively. IC(50) values for KYSE30 cell line were 500, 150, and 125 μg/ml after 24, 48, and 72 h, respectively. The EB/AO staining showed an increase in apoptotic cells. Cell cycle analysis showed a significant increase in cell density at G2/M phase. CONCLUSIONS: The results of the current study showed that the freeze-dried aqueous extract of E. elaterium fruit has a cytotoxic effect on gastric and esophageal cancer cell lines by means of apoptosis. The gastric cancer cells (AGS) showed a remarkably higher sensitivity. It seems that several compounds are possibly responsible for the cytotoxic effect of the extract.
PURPOSE:Ecballium elaterium (L.) A. Rich (Cucurbitaceae), also known as the "squirting cucumber," is a wild medicinal plant found abundantly in Moghan, Ardabil province, Iran. This study was undertaken to examine possible cytotoxic effect of freeze-dried aqueous extract of E. elaterium fruit on cell lines of gastric and esophageal origin namely called AGS (humangastric carcinoma) and KYSE30 (humanesophageal squamous cell carcinoma). METHODS: The aqueous extract of the fruits of E. elaterium was prepared and freeze-dried. AGS and KYSE30 cancer cell lines were treated by the extract and incubated for 24, 48, and 72 h. Cytotoxicity was examined by MTT assay. Ethidium bromide/acridine orange (EB/AO) staining was used for apoptotic cell detection. A DAPI staining method was used to analyze cell cycle by flow cytometry. RESULTS: The IC(50) values were 2.5, 0.7, and 0.7 μg/ml for AGS cell line after 24, 48, and 72 h, respectively. IC(50) values for KYSE30 cell line were 500, 150, and 125 μg/ml after 24, 48, and 72 h, respectively. The EB/AO staining showed an increase in apoptotic cells. Cell cycle analysis showed a significant increase in cell density at G2/M phase. CONCLUSIONS: The results of the current study showed that the freeze-dried aqueous extract of E. elaterium fruit has a cytotoxic effect on gastric and esophageal cancer cell lines by means of apoptosis. The gastric cancer cells (AGS) showed a remarkably higher sensitivity. It seems that several compounds are possibly responsible for the cytotoxic effect of the extract.
Authors: Adriana Miti Nakahata; Barbara Mayer; Christian Ries; Cláudia Alessandra Andrade de Paula; Marisa Karow; Peter Neth; Misako U Sampaio; Marianne Jochum; Maria Luiza V Oliva Journal: Biol Chem Date: 2011-04 Impact factor: 3.915
Authors: F Saidi; R Malekzadeh; M Sotoudeh; M H Derakhshan; M J Farahvash; A Yazdanbod; Sh Merat; J Mikaeli; R Sotoudehmanesh; S Nasseri-Moghadam; A Majidpour; S Arshi; B Abedi-Ardakani; A Yoonessi; F Sadr; A Sepehr; D Fleischer; S Fahimi Journal: Dis Esophagus Date: 2002 Impact factor: 3.429