Literature DB >> 1662809

Nuclease Bal-31 mapping of proteins bound to a tRNA(tyr) gene in SV40 minichromosomes.

S R Scanlon1, W R Folk.   

Abstract

We have analyzed proteins bound to active and to inactive tRNA(tyr) genes imbedded in the late coding region of SV40 minichromosomal DNA. Bal-31 nuclease resection from the 5' and 3' sides of the active tRNA(tyr) gene reveals proteins bound to the 5' flank, to the promoter 'A' block, to an intragenic sequence, to the promoter 'B' block and to a 3' downstream terminator/pause sequence. The proteins bound near the promoter 'B' block and the downstream terminator/pause sequence are reduced or eliminated by an inactivating deletion in the tRNA(tyr) 'B block'. That proteins are detected in the 5' flank and over the promoter 'A block' of the inactive gene contrasts with current notions regarding the requirement for a functional 'B' block for binding of transcription factors.

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Year:  1991        PMID： 1662809      PMCID： PMC332565          DOI： 10.1093/nar/19.25.7185

Source DB:  PubMed          Journal:  Nucleic Acids Res        ISSN： 0305-1048            Impact factor:   16.971


  37 in total

1.  Resolution of human transcription factor TFIIIC into two functional components.

Authors:  S K Yoshinaga; P A Boulanger; A J Berk
Journal:  Proc Natl Acad Sci U S A       Date:  1987-06       Impact factor: 11.205

2.  Analysis of RNA polymerase III transcription complexes by gel filtration.

Authors:  M F Carey; S P Gerrard; N R Cozzarelli
Journal:  J Biol Chem       Date:  1986-03-25       Impact factor: 5.157

3.  Association of RNA polymerase III with transcription factors in the absence of DNA.

Authors:  E Wingender; D Jahn; K H Seifart
Journal:  J Biol Chem       Date:  1986-01-25       Impact factor: 5.157

4.  Transcription complexes for various class III genes differ in parameters of formation and stability towards salt.

Authors:  D Jahn; E Wingender; K H Seifart
Journal:  J Mol Biol       Date:  1987-01-20       Impact factor: 5.469

5.  Comparison of tRNA gene transcription complexes formed in vitro and in nuclei.

Authors:  J M Huibregtse; C F Evans; D R Engelke
Journal:  Mol Cell Biol       Date:  1987-09       Impact factor: 4.272

6.  Genomic footprinting of a yeast tRNA gene reveals stable complexes over the 5'-flanking region.

Authors:  J M Huibregtse; D R Engelke
Journal:  Mol Cell Biol       Date:  1989-08       Impact factor: 4.272

7.  Identification of a protein factor binding to the 5'-flanking region of a tRNA gene and being involved in modulation of tRNA gene transcription in vivo in Saccharomyces cerevisiae.

Authors:  R Marschalek; T Dingermann
Journal:  Nucleic Acids Res       Date:  1988-07-25       Impact factor: 16.971

8.  Adenovirus stimulation of transcription by RNA polymerase III: evidence for an E1A-dependent increase in transcription factor IIIC concentration.

Authors:  S Yoshinaga; N Dean; M Han; A J Berk
Journal:  EMBO J       Date:  1986-02       Impact factor: 11.598

9.  The properties of a new polymerase III transcription factor reveal that transcription complexes can assemble by more than one pathway.

Authors:  S Ottonello; D H Rivier; G M Doolittle; L S Young; K U Sprague
Journal:  EMBO J       Date:  1987-07       Impact factor: 11.598

10.  Structural analysis of a triple complex between the histone octamer, a Xenopus gene for 5S RNA and transcription factor IIIA.

Authors:  D Rhodes
Journal:  EMBO J       Date:  1985-12-16       Impact factor: 11.598
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  1 in total

1.  Analysis of the role of 5' and 3' flanking sequence elements upon in vivo expression of the plant tRNATrp genes.

Authors:  B Ulmasov; W Folk
Journal:  Plant Cell       Date:  1995-10       Impact factor: 11.277
  1 in total

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