Literature DB >> 226980

Nicking-closing enzyme assembles nucleosome-like structures in vitro.

J E Germond, J Rouvière-Yaniv, M Yaniv, D Brutlag.   

Abstract

The four core histones (H2A, H2B, H3, and H4) and DNA were assembled into nucleosome-like particles at physiological ionic strengths either by an extract of chromatin rich in nicking-closing activity or by the purified nicking-closing enzyme itself. When histone-DNA complexes were assembled in vitro from relaxed circular DNA, nearly physiological numbers of superhelical turns were induced in the DNA molecule. Electron microscopy of the complexes assembled by the chromatin extract revealed a beaded structure and a reduction of the contour length compared to free DNA. Micrococcal nuclease digestion of the histone-DNA complexes yielded 145-base-pair DNA fragments typical of nucleosome core particles and shorter subnucleosomal DNA fragments of discrete length.

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Year:  1979        PMID： 226980      PMCID： PMC383917          DOI： 10.1073/pnas.76.8.3779

Source DB:  PubMed          Journal:  Proc Natl Acad Sci U S A        ISSN： 0027-8424            Impact factor:   11.205


  29 in total

1.  Folding of the DNA double helix in chromatin-like structures from simian virus 40.

Authors:  J E Germond; B Hirt; P Oudet; M Gross-Bellark; P Chambon
Journal:  Proc Natl Acad Sci U S A       Date:  1975-05       Impact factor: 11.205

2.  The problems of eukaryotic and prokaryotic DNA packaging and in vivo conformation posed by superhelix density heterogeneity.

Authors:  M Shure; D E Pulleyblank; J Vinograd
Journal:  Nucleic Acids Res       Date:  1977       Impact factor: 16.971

3.  Strand breakage by the DNA untwisting enzyme results in covalent attachment of the enzyme to DNA.

Authors:  J J Champoux
Journal:  Proc Natl Acad Sci U S A       Date:  1977-09       Impact factor: 11.205

4.  Purification and characterization of the DNA untwisting enzyme from rat liver.

Authors:  J J Champoux; B L McConaughy
Journal:  Biochemistry       Date:  1976-10-19       Impact factor: 3.162

5.  Stability of nucleosomes in native and reconstituted chromatins.

Authors:  J E Germond; M Bellard; P Oudet; P Chambon
Journal:  Nucleic Acids Res       Date:  1976-11       Impact factor: 16.971

6.  The nucleosome core protein.

Authors:  J O Thomas; P J Butler
Journal:  Cold Spring Harb Symp Quant Biol       Date:  1978

7.  Chromatin-like structures in polyoma virus and simian virus 10 lytic cycle.

Authors:  C Cremisi; P F Pignatti; O Croissant; M Yaniv
Journal:  J Virol       Date:  1975-01       Impact factor: 5.103

8.  E. coli DNA binding protein HU forms nucleosomelike structure with circular double-stranded DNA.

Authors:  J Rouvière-Yaniv; M Yaniv; J E Germond
Journal:  Cell       Date:  1979-06       Impact factor: 41.582

9.  Subunit structure of simian-virus-40 minichromosome.

Authors:  M Bellard; P Oudet; J E Germond; P Chambon
Journal:  Eur J Biochem       Date:  1976-11-15

10.  Assembly of SV40 chromatin in a cell-free system from Xenopus eggs.

Authors:  R A Laskey; A D Mills; N R Morris
Journal:  Cell       Date:  1977-02       Impact factor: 41.582
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  23 in total

1.  Extracts of Drosophila embryos mediate chromatin assembly in vitro.

Authors:  T Nelson; T S Hsieh; D Brutlag
Journal:  Proc Natl Acad Sci U S A       Date:  1979-11       Impact factor: 11.205

2.  In vitro chromatin assembly promoted by the Xenopus laevis S-150 cell-free extract is enhanced by treatment with RNase A.

Authors:  J M Sekiguchi; E B Kmiec
Journal:  Nucleic Acids Res       Date:  1992-02-25       Impact factor: 16.971

3.  Characterization of a histone-like protein extracted from yeast mitochondria.

Authors:  F Caron; C Jacq; J Rouvière-Yaniv
Journal:  Proc Natl Acad Sci U S A       Date:  1979-09       Impact factor: 11.205

4.  The HIR corepressor complex binds to nucleosomes generating a distinct protein/DNA complex resistant to remodeling by SWI/SNF.

Authors:  Philippe Prochasson; Laurence Florens; Selene K Swanson; Michael P Washburn; Jerry L Workman
Journal:  Genes Dev       Date:  2005-11-01       Impact factor: 11.361

5.  Assembly of nucleosome-like structures mediated by cauliflower DNA topoisomerase.

Authors:  H Fukata; K Ohgami; H Fukasawa
Journal:  Plant Mol Biol       Date:  1989-06       Impact factor: 4.076

6.  Initiation and bidirectional propagation of chromatin assembly from a target site for nucleotide excision repair.

Authors:  Pierre-Henri L Gaillard; J G Moggs; D M Roche; J P Quivy; P B Becker; R D Wood; G Almouzni
Journal:  EMBO J       Date:  1997-10-15       Impact factor: 11.598

7.  A CAF-1-PCNA-mediated chromatin assembly pathway triggered by sensing DNA damage.

Authors:  J G Moggs; P Grandi; J P Quivy; Z O Jónsson; U Hübscher; P B Becker; G Almouzni
Journal:  Mol Cell Biol       Date:  2000-02       Impact factor: 4.272

8.  The androgenic regulation of superhelical-DNA nicking-closing enzyme in rat ventral prostate.

Authors:  J D Filipenko; P S Rennie; N Bruchovsky
Journal:  Biochem J       Date:  1981-04-15       Impact factor: 3.857

9.  Polyoma virus minichromosomes: associated enzyme activities.

Authors:  B B Gourlie; V Pigiet; C B Breaux; M R Krauss; C R King; R M Benbow
Journal:  J Virol       Date:  1981-06       Impact factor: 5.103

10.  Differences in unwinding of supercoiled DNA induced by the two enantiomers of anti-benzo[a]pyrene diol epoxide.

Authors:  R Xu; S Birke; S E Carberry; N E Geacintov; C E Swenberg; R G Harvey
Journal:  Nucleic Acids Res       Date:  1992-12-11       Impact factor: 16.971
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