Literature DB >> 214759

The same amount of DNA is organized in in vitro-assembled nucleosomes irrespective of the origin of the histones.

C Spadafora, P Oudet, P Chambon.   

Abstract

The four histones H2A, H2B, H3 and H4 from calf thymus, CHO and sea urchin gastrula cells were associated by stepwise dialysis from 2 M NaCl with SV40 DNA Form I. The in vitro-assembled chromatins were visualized by electron microscopy and the size of the DNA fragments generated by digestion with DNase II was determined. Irrespective of the origin of the histones, the size of the smallest DNA band generated at early times of digestion was about 190 base pairs, whereas oligomeric DNA bands were multiples of 140 bp. These results support our previous proposal that the four histones H2A, H2B, H3 and H4 are able to organize more than 140 bp of DNA, but do not provide any evidence that the variability of histones H2A and H2B plays a role in the variability of the DNA repeat length of native chromatins.

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Year:  1978        PMID： 214759      PMCID： PMC342689          DOI： 10.1093/nar/5.10.3479

Source DB:  PubMed          Journal:  Nucleic Acids Res        ISSN： 0305-1048            Impact factor:   16.971


  17 in total

1.  Stability of nucleosomes in native and reconstituted chromatins.

Authors:  J E Germond; M Bellard; P Oudet; P Chambon
Journal:  Nucleic Acids Res       Date:  1976-11       Impact factor: 16.971

2.  Variation in chromatin structure in two cell types from the same tissue: a short DNA repeat length in cerebral cortex neurons.

Authors:  J O Thomas; R J Thompson
Journal:  Cell       Date:  1977-04       Impact factor: 41.582

3.  An octamer of histones in chromatin and free in solution.

Authors:  J O Thomas; R D Kornberg
Journal:  Proc Natl Acad Sci U S A       Date:  1975-07       Impact factor: 11.205

4.  Studies on acid hydrolases. I. A procedure for the preparation of acid deoxyribonuclease and other acid hydrolases.

Authors:  G Bernardi; A Bernardi; A Chersi
Journal:  Biochim Biophys Acta       Date:  1966-10-24

5.  Mammalian chromatin substructure studies with the calcium-magnesium endonuclease and two-dimensional polyacrylamide-gel electrophoresis.

Authors:  L A Burgoyne; D R Hewish; J Mobbs
Journal:  Biochem J       Date:  1974-10       Impact factor: 3.857

6.  Action of micrococcal nuclease on chromatin and the location of histone H1.

Authors:  M Noll; R D Kornberg
Journal:  J Mol Biol       Date:  1977-01-25       Impact factor: 5.469

7.  Biochemical evidence of variability in the DNA repeat length in the chromatin of higher eukaryotes.

Authors:  J L Compton; M Bellard; P Chambon
Journal:  Proc Natl Acad Sci U S A       Date:  1976-12       Impact factor: 11.205

8.  Closely spaced nucleosome cores in reconstituted histone.DNA complexes and histone-H1-depleted chromatin.

Authors:  M Steinmetz; R E Streeck; H G Zachau
Journal:  Eur J Biochem       Date:  1978-02

9.  Assembly of SV40 chromatin in a cell-free system from Xenopus eggs.

Authors:  R A Laskey; A D Mills; N R Morris
Journal:  Cell       Date:  1977-02       Impact factor: 41.582

10.  Differences and similarities in chromatin structure of Neurospora crassa and higher eucaryotes.

Authors:  M Noll
Journal:  Cell       Date:  1976-07       Impact factor: 41.582
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  6 in total

1.  HMG proteins (1 + 2) form beaded structures when complexed with closed circular DNA.

Authors:  D J Mathis; A Kindelis; C Spadafora
Journal:  Nucleic Acids Res       Date:  1980-06-25       Impact factor: 16.971

2.  Reversible dissociation of linker histone from chromatin with preservation of internucleosomal repeat.

Authors:  J Allan; D Z Staynov; H Gould
Journal:  Proc Natl Acad Sci U S A       Date:  1980-02       Impact factor: 11.205

3.  Self-assembly of single and closely spaced nucleosome core particles.

Authors:  M Noll; S Zimmer; A Engel; J Dubochet
Journal:  Nucleic Acids Res       Date:  1980-01-11       Impact factor: 16.971

4.  Complexes of the arginine-rich histone tetramer (H3)2(H4)2 with negatively supercoiled DNA: electron microscopy and chemical cross-linking.

Authors:  J O Thomas; P Oudet
Journal:  Nucleic Acids Res       Date:  1979-10-10       Impact factor: 16.971

5.  Nucleosome spacing generated by ISWI and CHD1 remodelers is constant regardless of nucleosome density.

Authors:  Corinna Lieleg; Philip Ketterer; Johannes Nuebler; Johanna Ludwigsen; Ulrich Gerland; Hendrik Dietz; Felix Mueller-Planitz; Philipp Korber
Journal:  Mol Cell Biol       Date:  2015-03-02       Impact factor: 4.272

6.  Nucleosome cores reconstituted from poly (dA-dT) and the octamer of histones.

Authors:  D Rhodes
Journal:  Nucleic Acids Res       Date:  1979       Impact factor: 16.971
  6 in total

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