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Literature DB >> 18784320

Nonutility of repeat laboratory testing for detection of Clostridium difficile by use of PCR or enzyme immunoassay.

Elisabeth Aichinger¹, Cathy D Schleck, William S Harmsen, Lisa M Nyre, Robin Patel.

Abstract

The diagnostic gains of repeat testing for Clostridium difficile by enzyme immunoassay and PCR (i.e., initial negative result followed by positive result) within a 7-day period were 1.9 and 1.7%, respectively. There is little value of repeat testing for C. difficile by enzyme immunoassay or PCR.

Entities: Species

Mesh：

Year: 2008 PMID： 18784320 PMCID： PMC2576580 DOI： 10.1128/JCM.00684-08

Source DB: PubMed Journal: J Clin Microbiol ISSN： 0095-1137 Impact factor: 5.948

16 in total

Review 1. Clinical practice. Antibiotic-associated diarrhea.

Authors: John G Bartlett
Journal: N Engl J Med Date: 2002-01-31 Impact factor: 91.245

2. Frequency of sample submission for optimal utilization of the cell culture cytotoxicity assay for detection of Clostridium difficile toxin.

Authors: Anita P Borek; Deborah Z Aird; Karen C Carroll
Journal: J Clin Microbiol Date: 2005-06 Impact factor: 5.948

3. Evaluation of real-time PCR and conventional diagnostic methods for the detection of Clostridium difficile-associated diarrhoea in a prospective multicentre study.

Authors: Renate J van den Berg; Norbert Vaessen; Hubert P Endtz; Tanja Schülin; Eric R van der Vorm; Ed J Kuijper
Journal: J Med Microbiol Date: 2007-01 Impact factor: 2.472

4. Development and evaluation of a PCR method for detection of the Clostridium difficile toxin B gene in stool specimens.

Authors: C Guilbault; A-C Labbé; L Poirier; L Busque; C Béliveau; M Laverdière
Journal: J Clin Microbiol Date: 2002-06 Impact factor: 5.948

Review 5. Guidelines for the diagnosis and management of Clostridium difficile-associated diarrhea and colitis. American College of Gastroenterology, Practice Parameters Committee.

Authors: R Fekety
Journal: Am J Gastroenterol Date: 1997-05 Impact factor: 10.864

6. Lack of value of repeat stool testing for Clostridium difficile toxin.

Authors: Sowjanya S Mohan; Brian P McDermott; Subha Parchuri; Burke A Cunha
Journal: Am J Med Date: 2006-04 Impact factor: 4.965

7. Detection of toxigenic Clostridium difficile in stool samples by real-time polymerase chain reaction for the diagnosis of C. difficile-associated diarrhea.

Authors: Lance R Peterson; Rebecca U Manson; Suzanne M Paule; Donna M Hacek; Ari Robicsek; Richard B Thomson; Karen L Kaul
Journal: Clin Infect Dis Date: 2007-09-25 Impact factor: 9.079

8. Comparison of real-time PCR for detection of the tcdC gene with four toxin immunoassays and culture in diagnosis of Clostridium difficile infection.

Authors: Lynne M Sloan; Brian J Duresko; Daniel R Gustafson; Jon E Rosenblatt
Journal: J Clin Microbiol Date: 2008-04-23 Impact factor: 5.948

9. The lack of value of repeated Clostridium difficile cytotoxicity assays.

Authors: A A Renshaw; J M Stelling; M H Doolittle
Journal: Arch Pathol Lab Med Date: 1996-01 Impact factor: 5.534

10. Effect on diagnostic yield of repeated stool testing during outbreaks of Clostridium difficile-associated disease.

Authors: S B Debast; E van Kregten; K M G Oskam; T van den Berg; R J Van den Berg; E J Kuijper
Journal: Clin Microbiol Infect Date: 2008-04-03 Impact factor: 8.067

31 in total

1. A multiplex, internally controlled real-time PCR assay for detection of toxigenic Clostridium difficile and identification of hypervirulent strain 027/ST-1.

Authors: A M Hoegh; J B Nielsen; A Lester; A Friis-Møller; K Schønning
Journal: Eur J Clin Microbiol Infect Dis Date: 2011-09-22 Impact factor: 3.267

2. Reducing Unnecessary and Duplicate Ordering for Ovum and Parasite Examinations and Clostridium difficile PCR in Immunocompromised Patients by Using an Alert at the Time of Request in the Order Management System.

Authors: Caitlin C Otto; Susan L Shuptar; Philippe Milord; Connor J Essick; Reshma Nevrekar; Svetlana L Granovsky; Susan K Seo; N Esther Babady; Steven C Martin; Yi-Wei Tang; Melissa S Pessin
Journal: J Clin Microbiol Date: 2015-06-10 Impact factor: 5.948

Review 3. Laboratory diagnosis of bacterial gastroenteritis.

Authors: Romney M Humphries; Andrea J Linscott
Journal: Clin Microbiol Rev Date: 2015-01 Impact factor: 26.132

4. Characteristics and Antibiotic Use Associated With Short-Term Risk of Clostridium difficile Infection Among Hospitalized Patients.

Authors: Sol del Mar Aldrete; Matthew J Magee; Rachel J Friedman-Moraco; Austin W Chan; Grier G Banks; Eileen M Burd; Colleen S Kraft
Journal: Am J Clin Pathol Date: 2015-06 Impact factor: 2.493

5. Role of fecal Clostridium difficile load in discrepancies between toxin tests and PCR: is quantitation the next step in C. difficile testing?

Authors: J L Leslie; S H Cohen; J V Solnick; C R Polage
Journal: Eur J Clin Microbiol Infect Dis Date: 2012-07-20 Impact factor: 3.267

10. Evaluation of tcdB real-time PCR in a three-step diagnostic algorithm for detection of toxigenic Clostridium difficile.

Authors: Ann M Larson; Angela M Fung; Ferric C Fang
Journal: J Clin Microbiol Date: 2009-11-18 Impact factor: 5.948