Literature DB >> 18757857

Doc of prophage P1 is inhibited by its antitoxin partner Phd through fold complementation.

Abel Garcia-Pino1, Mikkel Christensen-Dalsgaard, Lode Wyns, Michael Yarmolinsky, Roy David Magnuson, Kenn Gerdes, Remy Loris.   

Abstract

Prokaryotic toxin-antitoxin modules are involved in major physiological events set in motion under stress conditions. The toxin Doc (death on curing) from the phd/doc module on phage P1 hosts the C-terminal domain of its antitoxin partner Phd (prevents host death) through fold complementation. This Phd domain is intrinsically disordered in solution and folds into an alpha-helix upon binding to Doc. The details of the interactions reveal the molecular basis for the inhibitory action of the antitoxin. The complex resembles the Fic (filamentation induced by cAMP) proteins and suggests a possible evolutionary origin for the phd/doc operon. Doc induces growth arrest of Escherichia coli cells in a reversible manner, by targeting the protein synthesis machinery. Moreover, Doc activates the endogenous E. coli RelE mRNA interferase but does not require this or any other known chromosomal toxin-antitoxin locus for its action in vivo.

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Year:  2008        PMID： 18757857      PMCID： PMC2576525          DOI： 10.1074/jbc.M805654200

Source DB:  PubMed          Journal:  J Biol Chem        ISSN： 0021-9258            Impact factor:   5.157


  40 in total

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Authors:  F G Sauer; K Fütterer; J S Pinkner; K W Dodson; S J Hultgren; G Waksman
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2.  The Doc toxin and Phd antidote proteins of the bacteriophage P1 plasmid addiction system form a heterotrimeric complex.

Authors:  E Gazit; R T Sauer
Journal:  J Biol Chem       Date:  1999-06-11       Impact factor: 5.157

3.  Substructure solution with SHELXD.

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4.  Rapid induction and reversal of a bacteriostatic condition by controlled expression of toxins and antitoxins.

Authors:  Kim Pedersen; Susanne K Christensen; Kenn Gerdes
Journal:  Mol Microbiol       Date:  2002-07       Impact factor: 3.501

5.  Postsegregational killing mediated by the P1 phage "addiction module" phd-doc requires the Escherichia coli programmed cell death system mazEF.

Authors:  R Hazan; B Sat; M Reches; H Engelberg-Kulka
Journal:  J Bacteriol       Date:  2001-03       Impact factor: 3.490

6.  A novel main-chain anion-binding site in proteins: the nest. A particular combination of phi,psi values in successive residues gives rise to anion-binding sites that occur commonly and are found often at functionally important regions.

Authors:  James D Watson; E James Milner-White
Journal:  J Mol Biol       Date:  2002-01-11       Impact factor: 5.469

7.  RelE toxins from bacteria and Archaea cleave mRNAs on translating ribosomes, which are rescued by tmRNA.

Authors:  Susanne K Christensen; Kenn Gerdes
Journal:  Mol Microbiol       Date:  2003-06       Impact factor: 3.501

8.  Crystal structure of the intrinsically flexible addiction antidote MazE.

Authors:  Remy Loris; Irina Marianovsky; Jurij Lah; Toon Laeremans; Hanna Engelberg-Kulka; Gad Glaser; Serge Muyldermans; Lode Wyns
Journal:  J Biol Chem       Date:  2003-05-12       Impact factor: 5.157

9.  MazF cleaves cellular mRNAs specifically at ACA to block protein synthesis in Escherichia coli.

Authors:  Yonglong Zhang; Junjie Zhang; Klaus P Hoeflich; Mitsuhiko Ikura; Guoliang Qing; Masayori Inouye
Journal:  Mol Cell       Date:  2003-10       Impact factor: 17.970

10.  New connections in the prokaryotic toxin-antitoxin network: relationship with the eukaryotic nonsense-mediated RNA decay system.

Authors:  Vivek Anantharaman; L Aravind
Journal:  Genome Biol       Date:  2003-11-26       Impact factor: 13.583
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  62 in total

1.  Functional equivalence and evolutionary convergence in complex communities of microbial sponge symbionts.

Authors:  Lu Fan; David Reynolds; Michael Liu; Manuel Stark; Staffan Kjelleberg; Nicole S Webster; Torsten Thomas
Journal:  Proc Natl Acad Sci U S A       Date:  2012-06-13       Impact factor: 11.205

2.  The ParE2-PaaA2 toxin-antitoxin complex from Escherichia coli O157 forms a heterodocecamer in solution and in the crystal.

Authors:  Yann G J Sterckx; Abel Garcia-Pino; Sarah Haesaerts; Thomas Jové; Lieselotte Geerts; Viktor Sakellaris; Laurence Van Melderen; Remy Loris
Journal:  Acta Crystallogr Sect F Struct Biol Cryst Commun       Date:  2012-05-25

3.  Crystal structures of Phd-Doc, HigA, and YeeU establish multiple evolutionary links between microbial growth-regulating toxin-antitoxin systems.

Authors:  Mark A Arbing; Samuel K Handelman; Alexandre P Kuzin; Grégory Verdon; Chi Wang; Min Su; Francesca P Rothenbacher; Mariam Abashidze; Mohan Liu; Jennifer M Hurley; Rong Xiao; Thomas Acton; Masayori Inouye; Gaetano T Montelione; Nancy A Woychik; John F Hunt
Journal:  Structure       Date:  2010-08-11       Impact factor: 5.006

4.  HicA of Escherichia coli defines a novel family of translation-independent mRNA interferases in bacteria and archaea.

Authors:  Mikkel G Jørgensen; Deo P Pandey; Milena Jaskolska; Kenn Gerdes
Journal:  J Bacteriol       Date:  2008-12-05       Impact factor: 3.490

5.  Crystallization of the HigBA2 toxin-antitoxin complex from Vibrio cholerae.

Authors:  San Hadži; Abel Garcia-Pino; Sergio Martinez-Rodriguez; Koen Verschueren; Mikkel Christensen-Dalsgaard; Kenn Gerdes; Jurij Lah; Remy Loris
Journal:  Acta Crystallogr Sect F Struct Biol Cryst Commun       Date:  2013-08-27

6.  Molecular mechanisms of multiple toxin-antitoxin systems are coordinated to govern the persister phenotype.

Authors:  Rick A Fasani; Michael A Savageau
Journal:  Proc Natl Acad Sci U S A       Date:  2013-06-18       Impact factor: 11.205

7.  Purification and crystallization of Phd, the antitoxin of the phd/doc operon.

Authors:  Abel Garcia-Pino; Yann Sterckx; Guy Vandenbussche; Remy Loris
Journal:  Acta Crystallogr Sect F Struct Biol Cryst Commun       Date:  2010-01-27

8.  The intrinsically disordered domain of the antitoxin Phd chaperones the toxin Doc against irreversible inactivation and misfolding.

Authors:  Steven De Gieter; Albert Konijnenberg; Ariel Talavera; Annika Butterer; Sarah Haesaerts; Henri De Greve; Frank Sobott; Remy Loris; Abel Garcia-Pino
Journal:  J Biol Chem       Date:  2014-10-16       Impact factor: 5.157

9.  AtaT blocks translation initiation by N-acetylation of the initiator tRNAfMet.

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10.  Three new RelE-homologous mRNA interferases of Escherichia coli differentially induced by environmental stresses.

Authors:  Mikkel Christensen-Dalsgaard; Mikkel Girke Jørgensen; Kenn Gerdes
Journal:  Mol Microbiol       Date:  2009-11-25       Impact factor: 3.501
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