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Literature DB >> 20124714

Purification and crystallization of Phd, the antitoxin of the phd/doc operon.

Abel Garcia-Pino¹, Yann Sterckx, Guy Vandenbussche, Remy Loris.

Abstract

The antitoxin Phd from the phd/doc module of bacteriophage P1 was crystallized in two distinct crystal forms. Crystals of His-tagged Phd contain a C-terminally truncated version of the protein and diffract to 2.20 A resolution. Crystals of untagged Phd purified from the Phd-Doc complex diffract to 2.25 A resolution. These crystals are partially merohedrally twinned and contain the full-length version of the protein.

Entities: Species

Mesh：

Substances：
Antitoxins
Viral Proteins

Year: 2010 PMID： 20124714 PMCID： PMC2815684 DOI： 10.1107/S1744309109051550

Source DB: PubMed Journal: Acta Crystallogr Sect F Struct Biol Cryst Commun ISSN： 1744-3091

28 in total

Review 1. Prokaryotic toxin-antitoxin stress response loci.

Authors: Kenn Gerdes; Susanne K Christensen; Anders Løbner-Olesen
Journal: Nat Rev Microbiol Date: 2005-05 Impact factor: 60.633

2. Conformational change in the catalytic site of the ribonuclease YoeB toxin by YefM antitoxin.

Authors: Katsuhiko Kamada; Fumio Hanaoka
Journal: Mol Cell Date: 2005-08-19 Impact factor: 17.970

3. Two higBA loci in the Vibrio cholerae superintegron encode mRNA cleaving enzymes and can stabilize plasmids.

Authors: Mikkel Christensen-Dalsgaard; Kenn Gerdes
Journal: Mol Microbiol Date: 2006-10 Impact factor: 3.501

4. Crystal structure of archaeal toxin-antitoxin RelE-RelB complex with implications for toxin activity and antitoxin effects.

Authors: Hisanori Takagi; Yoshimitsu Kakuta; Takahiro Okada; Min Yao; Isao Tanaka; Makoto Kimura
Journal: Nat Struct Mol Biol Date: 2005-03-13 Impact factor: 15.369

5. Toxin-antitoxin loci as stress-response-elements: ChpAK/MazF and ChpBK cleave translated RNAs and are counteracted by tmRNA.

Authors: Susanne K Christensen; Kim Pedersen; Flemming G Hansen; Kenn Gerdes
Journal: J Mol Biol Date: 2003-09-26 Impact factor: 5.469

6. Structure and proposed activity of a member of the VapBC family of toxin-antitoxin systems. VapBC-5 from Mycobacterium tuberculosis.

Authors: Linda Miallau; Michael Faller; Janet Chiang; Mark Arbing; Feng Guo; Duilio Cascio; David Eisenberg
Journal: J Biol Chem Date: 2008-10-24 Impact factor: 5.157

Purification and crystallization of Phd, the antitoxin of the phd/doc operon.

Review 1. Prokaryotic toxin-antitoxin stress response loci.

2. Conformational change in the catalytic site of the ribonuclease YoeB toxin by YefM antitoxin.

3. Two higBA loci in the Vibrio cholerae superintegron encode mRNA cleaving enzymes and can stabilize plasmids.

4. Crystal structure of archaeal toxin-antitoxin RelE-RelB complex with implications for toxin activity and antitoxin effects.

5. Toxin-antitoxin loci as stress-response-elements: ChpAK/MazF and ChpBK cleave translated RNAs and are counteracted by tmRNA.

6. Structure and proposed activity of a member of the VapBC family of toxin-antitoxin systems. VapBC-5 from Mycobacterium tuberculosis.

7. Involvement of cyclic AMP and its receptor protein in filamentation of an Escherichia coli fic mutant.

8. Crystal structure of the intrinsically flexible addiction antidote MazE.

9. Doc of prophage P1 is inhibited by its antitoxin partner Phd through fold complementation.

10. MazF cleaves cellular mRNAs specifically at ACA to block protein synthesis in Escherichia coli.

1. Crystallization and X-ray analysis of all of the players in the autoregulation of the ataRT toxin-antitoxin system.