Literature DB >> 18723655

Development of a transposon mutagenesis system in the oral spirochete Treponema denticola.

Yu Yang1, Philip E Stewart, Xiaoguang Shi, Chunhao Li.   

Abstract

Here, we report successful transposon mutagenesis in the oral spirochete Treponema denticola. A modified Himar1 transposon, including a new antibiotic selection cassette for T. denticola, generated mutations affecting cell division, transport, and chemotaxis, among other processes. This random mutagenesis system should facilitate research on the biology and pathogenesis of this spirochete, which is associated with human periodontal diseases.

Entities:  

Mesh：

Substances：

Year:  2008        PMID： 18723655      PMCID： PMC2570287          DOI： 10.1128/AEM.01424-08

Source DB:  PubMed          Journal:  Appl Environ Microbiol        ISSN： 0099-2240            Impact factor:   4.792


  29 in total

1.  Selection for in vivo regulators of bacterial virulence.

Authors:  S H Lee; S M Butler; A Camilli
Journal:  Proc Natl Acad Sci U S A       Date:  2001-06-05       Impact factor: 11.205

2.  Virulence factors of oral treponemes.

Authors:  J C Fenno; B C McBride
Journal:  Anaerobe       Date:  1998-02       Impact factor: 3.331

Review 3.  Isolation of transposon insertions.

Authors:  Stanley R Maloy
Journal:  Methods Enzymol       Date:  2007       Impact factor: 1.600

Review 4.  Bacterial genomics and pathogen evolution.

Authors:  David M Raskin; Rekha Seshadri; Stefan U Pukatzki; John J Mekalanos
Journal:  Cell       Date:  2006-02-24       Impact factor: 41.582

5.  Gene inactivation in the oral spirochete Treponema denticola: construction of an flgE mutant.

Authors:  H Li; J Ruby; N Charon; H Kuramitsu
Journal:  J Bacteriol       Date:  1996-06       Impact factor: 3.490

6.  Hyperactive transposase mutants of the Himar1 mariner transposon.

Authors:  D J Lampe; B J Akerley; E J Rubin; J J Mekalanos; H M Robertson
Journal:  Proc Natl Acad Sci U S A       Date:  1999-09-28       Impact factor: 11.205

7.  Investigation of the catalytic site within the ATP-grasp domain of Clostridium symbiosum pyruvate phosphate dikinase.

Authors:  D Ye; M Wei; M McGuire; K Huang; G Kapadia; O Herzberg; B M Martin; D Dunaway-Mariano
Journal:  J Biol Chem       Date:  2001-07-23       Impact factor: 5.157

8.  Random insertional mutagenesis of Leptospira interrogans, the agent of leptospirosis, using a mariner transposon.

Authors:  Pascale Bourhy; Hélène Louvel; Isabelle Saint Girons; Mathieu Picardeau
Journal:  J Bacteriol       Date:  2005-05       Impact factor: 3.490

9.  Attenuated signature-tagged mutagenesis mutants of Brucella melitensis identified during the acute phase of infection in mice.

Authors:  P Lestrate; A Dricot; R-M Delrue; C Lambert; V Martinelli; X De Bolle; J-J Letesson; A Tibor
Journal:  Infect Immun       Date:  2003-12       Impact factor: 3.441

10.  Genome-wide transposon mutagenesis of Borrelia burgdorferi for identification of phenotypic mutants.

Authors:  Philip E Stewart; Jessica Hoff; Elizabeth Fischer; Jonathan G Krum; Patricia A Rosa
Journal:  Appl Environ Microbiol       Date:  2004-10       Impact factor: 4.792
View more
  13 in total

1.  Development of a modified gentamicin resistance cassette for genetic manipulation of the oral spirochete Treponema denticola.

Authors:  Jiang Bian; J Christopher Fenno; Chunhao Li
Journal:  Appl Environ Microbiol       Date:  2012-01-13       Impact factor: 4.792

2.  Disruption of a type II endonuclease (TDE0911) enables Treponema denticola ATCC 35405 to accept an unmethylated shuttle vector.

Authors:  Jiang Bian; Chunhao Li
Journal:  Appl Environ Microbiol       Date:  2011-05-20       Impact factor: 4.792

3.  The riboswitch regulates a thiamine pyrophosphate ABC transporter of the oral spirochete Treponema denticola.

Authors:  Jiang Bian; Hongwu Shen; Youbin Tu; Aiming Yu; Chunhao Li
Journal:  J Bacteriol       Date:  2011-05-27       Impact factor: 3.490

4.  pyrF as a Counterselectable Marker for Unmarked Genetic Manipulations in Treponema denticola.

Authors:  Kurni Kurniyati; Chunhao Li
Journal:  Appl Environ Microbiol       Date:  2015-12-18       Impact factor: 4.792

5.  Cellular architecture of Treponema pallidum: novel flagellum, periplasmic cone, and cell envelope as revealed by cryo electron tomography.

Authors:  Jun Liu; Jerrilyn K Howell; Sherille D Bradley; Yesha Zheng; Z Hong Zhou; Steven J Norris
Journal:  J Mol Biol       Date:  2010-09-17       Impact factor: 5.469

6.  Kanamycin Resistance Cassette for Genetic Manipulation of Treponema denticola.

Authors:  Yuebin Li; John Ruby; Hui Wu
Journal:  Appl Environ Microbiol       Date:  2015-04-17       Impact factor: 4.792

7.  A simplified erythromycin resistance cassette for Treponema denticola mutagenesis.

Authors:  M Paula Goetting-Minesky; J Christopher Fenno
Journal:  J Microbiol Methods       Date:  2010-08-04       Impact factor: 2.363

8.  Identification of genes involved in biofilm formation and respiration via mini-Himar transposon mutagenesis of Geobacter sulfurreducens.

Authors:  Janet B Rollefson; Caleb E Levar; Daniel R Bond
Journal:  J Bacteriol       Date:  2009-04-24       Impact factor: 3.490

Review 9.  Transposition of fly mariner elements into bacteria as a genetic tool for mutagenesis.

Authors:  Mathieu Picardeau
Journal:  Genetica       Date:  2009-09-13       Impact factor: 1.082

10.  Genetic Manipulations of Oral Spirochete Treponema denticola.

Authors:  Kurni Kurniyati; Chunhao Li
Journal:  Methods Mol Biol       Date:  2021
View more

北京卡尤迪生物科技股份有限公司 © 2022-2023.