Literature DB >> 20691222

A simplified erythromycin resistance cassette for Treponema denticola mutagenesis.

M Paula Goetting-Minesky1, J Christopher Fenno.   

Abstract

The primary selectable marker for the genetic studies of Treponema denticola is a hybrid gene cassette containing both ermF and ermAM (ermB) genes. ErmB functions in Escherichia coli, while ErmF has been assumed to confer resistance in T. denticola. We demonstrate here that ErmB is sufficient for erythromycin selection in T. denticola and that the native ermB promoter drives ErmB expression.
Copyright © 2010 Elsevier B.V. All rights reserved.

Entities:  

Mesh：

Substances：

Year:  2010        PMID： 20691222      PMCID： PMC2953711          DOI： 10.1016/j.mimet.2010.07.020

Source DB:  PubMed          Journal:  J Microbiol Methods        ISSN： 0167-7012            Impact factor:   2.363


  28 in total

1.  Native plasmids of Fusobacterium nucleatum: characterization and use in development of genetic systems.

Authors:  S K Haake; S C Yoder; G Attarian; K Podkaminer
Journal:  J Bacteriol       Date:  2000-02       Impact factor: 3.490

2.  Treponema denticola PrcB is required for expression and activity of the PrcA-PrtP (dentilisin) complex.

Authors:  Valentina Godovikova; Hong-Tao Wang; M Paula Goetting-Minesky; Yu Ning; Ricardo F Capone; Claudia K Slater; J Christopher Fenno
Journal:  J Bacteriol       Date:  2010-04-30       Impact factor: 3.490

3.  Inhibition of growth of treponemes by antimicrobial agents.

Authors:  I J Abramson; R M Smibert
Journal:  Br J Vener Dis       Date:  1971-12

Review 4.  Acquired antibiotic resistance in lactic acid bacteria from food.

Authors:  M Teuber; L Meile; F Schwarz
Journal:  Antonie Van Leeuwenhoek       Date:  1999 Jul-Nov       Impact factor: 2.271

5.  Complementation of a Treponema denticola flgE mutant with a novel coumermycin A1-resistant T. denticola shuttle vector system.

Authors:  Bo Chi; Ronald J Limberger; Howard K Kuramitsu
Journal:  Infect Immun       Date:  2002-04       Impact factor: 3.441

6.  Flavobacterium johnsoniae GldH is a lipoprotein that is required for gliding motility and chitin utilization.

Authors:  Mark J McBride; Timothy F Braun; Jessica L Brust
Journal:  J Bacteriol       Date:  2003-11       Impact factor: 3.490

7.  Development of a novel chloramphenicol resistance expression plasmid used for genetic complementation of a fliG deletion mutant in Treponema denticola.

Authors:  Linda L Slivienski-Gebhardt; Jacques Izard; William A Samsonoff; Ronald J Limberger
Journal:  Infect Immun       Date:  2004-09       Impact factor: 3.441

8.  Development of genetic techniques for the psychrotrophic fish pathogen Flavobacterium psychrophilum.

Authors:  B Alvarez; P Secades; M J McBride; J A Guijarro
Journal:  Appl Environ Microbiol       Date:  2004-01       Impact factor: 4.792

9.  Cleavage of Treponema denticola PrcA polypeptide to yield protease complex-associated proteins Prca1 and Prca2 is dependent on PrtP.

Authors:  Si Young Lee; Xue-Lin Bian; Grace W K Wong; Pauline M Hannam; Barry C McBride; J Christopher Fenno
Journal:  J Bacteriol       Date:  2002-07       Impact factor: 3.490

10.  Comparison of the genome of the oral pathogen Treponema denticola with other spirochete genomes.

Authors:  Rekha Seshadri; Garry S A Myers; Hervé Tettelin; Jonathan A Eisen; John F Heidelberg; Robert J Dodson; Tanja M Davidsen; Robert T DeBoy; Derrick E Fouts; Dan H Haft; Jeremy Selengut; Qinghu Ren; Lauren M Brinkac; Ramana Madupu; Jamie Kolonay; Scott A Durkin; Sean C Daugherty; Jyoti Shetty; Alla Shvartsbeyn; Elizabeth Gebregeorgis; Keita Geer; Getahun Tsegaye; Joel Malek; Bola Ayodeji; Sofiya Shatsman; Michael P McLeod; David Smajs; Jerrilyn K Howell; Sangita Pal; Anita Amin; Pankaj Vashisth; Thomas Z McNeill; Qin Xiang; Erica Sodergren; Ernesto Baca; George M Weinstock; Steven J Norris; Claire M Fraser; Ian T Paulsen
Journal:  Proc Natl Acad Sci U S A       Date:  2004-04-02       Impact factor: 11.205
View more
  17 in total

1.  Development of a modified gentamicin resistance cassette for genetic manipulation of the oral spirochete Treponema denticola.

Authors:  Jiang Bian; J Christopher Fenno; Chunhao Li
Journal:  Appl Environ Microbiol       Date:  2012-01-13       Impact factor: 4.792

2.  1,2-Diacylglycerol choline phosphotransferase catalyzes the final step in the unique Treponema denticola phosphatidylcholine biosynthesis pathway.

Authors:  Miguel Ángel Vences-Guzmán; M Paula Goetting-Minesky; Ziqiang Guan; Santiago Castillo-Ramirez; Luz América Córdoba-Castro; Isabel M López-Lara; Otto Geiger; Christian Sohlenkamp; J Christopher Fenno
Journal:  Mol Microbiol       Date:  2017-01-17       Impact factor: 3.501

3.  Roles of TroA and TroR in Metalloregulated Growth and Gene Expression in Treponema denticola.

Authors:  Prakaimuk Saraithong; M Paula Goetting-Minesky; Peter M Durbin; Spencer W Olson; Frank C Gherardini; J Christopher Fenno
Journal:  J Bacteriol       Date:  2020-03-11       Impact factor: 3.490

4.  Disruption of a type II endonuclease (TDE0911) enables Treponema denticola ATCC 35405 to accept an unmethylated shuttle vector.

Authors:  Jiang Bian; Chunhao Li
Journal:  Appl Environ Microbiol       Date:  2011-05-20       Impact factor: 4.792

5.  pyrF as a Counterselectable Marker for Unmarked Genetic Manipulations in Treponema denticola.

Authors:  Kurni Kurniyati; Chunhao Li
Journal:  Appl Environ Microbiol       Date:  2015-12-18       Impact factor: 4.792

6.  Kanamycin Resistance Cassette for Genetic Manipulation of Treponema denticola.

Authors:  Yuebin Li; John Ruby; Hui Wu
Journal:  Appl Environ Microbiol       Date:  2015-04-17       Impact factor: 4.792

7.  Evidence that TP_0144 of Treponema pallidum is a thiamine-binding protein.

Authors:  Jiang Bian; Youbin Tu; Song-Mei Wang; Xuan-Yi Wang; Chunhao Li
Journal:  J Bacteriol       Date:  2015-01-20       Impact factor: 3.490

8.  A novel glycan modifies the flagellar filament proteins of the oral bacterium Treponema denticola.

Authors:  Kurni Kurniyati; John F Kelly; Evgeny Vinogradov; Anna Robotham; Youbing Tu; Juyu Wang; Jun Liu; Susan M Logan; Chunhao Li
Journal:  Mol Microbiol       Date:  2016-10-27       Impact factor: 3.501

9.  Genetic Manipulations of Oral Spirochete Treponema denticola.

Authors:  Kurni Kurniyati; Chunhao Li
Journal:  Methods Mol Biol       Date:  2021

10.  A pleiotropic role of FlaG in regulating the cell morphogenesis and flagellar homeostasis at the cell poles of Treponema denticola.

Authors:  Kurni Kurniyati; Jun Liu; Jing-Ren Zhang; Yunjiang Min; Chunhao Li
Journal:  Cell Microbiol       Date:  2018-07-23       Impact factor: 3.715
View more

北京卡尤迪生物科技股份有限公司 © 2022-2023.